Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing

Background Intracoronary provocation testing with acetylcholine (ACh) is helpful to diagnose and risk‐stratify patients with ischemia with nonobstructed coronary arteries (NOCA) and myocardial infarction with NOCA. This study explored potential sex‐related disparities on the prognostic significance...

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Main Authors: Riccardo Rinaldi, Michele Russo, Giovanni Occhipinti, Claudio Laudani, Ilaria Torre, Michele Colucci, Filippo Luca Gurgoglione, Francesco Maria Animati, Jacopo Lenkowicz, Andrada Mihaela Tudor, Giovanna Liuzzo, Tommaso Sanna, Antonio Maria Leone, Giampaolo Niccoli, Gaetano A. Lanza, Carlo Trani, Francesco Burzotta, Filippo Crea, Rocco A. Montone
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
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Online Access:https://www.ahajournals.org/doi/10.1161/JAHA.124.037942
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author Riccardo Rinaldi
Michele Russo
Giovanni Occhipinti
Claudio Laudani
Ilaria Torre
Michele Colucci
Filippo Luca Gurgoglione
Francesco Maria Animati
Jacopo Lenkowicz
Andrada Mihaela Tudor
Giovanna Liuzzo
Tommaso Sanna
Antonio Maria Leone
Giampaolo Niccoli
Gaetano A. Lanza
Carlo Trani
Francesco Burzotta
Filippo Crea
Rocco A. Montone
author_facet Riccardo Rinaldi
Michele Russo
Giovanni Occhipinti
Claudio Laudani
Ilaria Torre
Michele Colucci
Filippo Luca Gurgoglione
Francesco Maria Animati
Jacopo Lenkowicz
Andrada Mihaela Tudor
Giovanna Liuzzo
Tommaso Sanna
Antonio Maria Leone
Giampaolo Niccoli
Gaetano A. Lanza
Carlo Trani
Francesco Burzotta
Filippo Crea
Rocco A. Montone
author_sort Riccardo Rinaldi
collection DOAJ
description Background Intracoronary provocation testing with acetylcholine (ACh) is helpful to diagnose and risk‐stratify patients with ischemia with nonobstructed coronary arteries (NOCA) and myocardial infarction with NOCA. This study explored potential sex‐related disparities on the prognostic significance of ACh provocative testing. Methods Consecutive patients with ischemia with NOCA and those with myocardial infarction with NOCA who underwent ACh provocation testing were enrolled. The primary end point was the incidence of major adverse cardiovascular and cerebrovascular events at follow‐up. Co‐primary end points were angina recurrence and quality of life assessed by 12‐month Seattle Angina Questionnaire (SAQ) summary score. Results A total of 519 patients (mean age, 61.4±12.1 years; 275 [53.0%] women and 244 [47%] men) were enrolled: 346 (66.7%) with ischemia with NOCA and 173 (33.3%) with myocardial infarction with NOCA. A positive ACh test was observed in 274 (52.8%) patients, with a lower prevalence of epicardial spasm (82 [56.2%] versus 106 [82.8%]) and a higher prevalence of microvascular spasm (64 [43.8%] versus 22 [17.2%]) in women compared with men (P>0.001). After a median 22‐month follow‐up, major adverse cardiovascular and cerebrovascular events occurred in 53 (10.2%) patients, without significant sex differences (P>0.05). Men with a positive ACh test had a significantly higher rate of major adverse cardiovascular and cerebrovascular events (22 [17.2%] versus 5 [4.3%], P=0.002) compared with those with a negative test; no difference was observed in women (P>0.05) (P for interaction=0.003). Women with a positive test experienced a higher rate of angina recurrence (61 [41.8%] versus 32 [24.8%], P=0.005) and a lower SAQ summary score (82 [interquartile range, 72–90] versus 86 [interquartile range, 78–100], P<0.001) compared with those with a negative result; no difference was observed in men (P>0.05). Conclusions This study revealed the importance of recognizing sex‐specific differences in the prognostic value of ACh testing for proper management of coronary vasomotor disorders.
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spelling doaj-art-00a5572969584b6890dcf57d085476d52025-08-20T02:24:59ZengWileyJournal of the American Heart Association: Cardiovascular and Cerebrovascular Disease2047-99802025-03-0114510.1161/JAHA.124.037942Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation TestingRiccardo Rinaldi0Michele Russo1Giovanni Occhipinti2Claudio Laudani3Ilaria Torre4Michele Colucci5Filippo Luca Gurgoglione6Francesco Maria Animati7Jacopo Lenkowicz8Andrada Mihaela Tudor9Giovanna Liuzzo10Tommaso Sanna11Antonio Maria Leone12Giampaolo Niccoli13Gaetano A. Lanza14Carlo Trani15Francesco Burzotta16Filippo Crea17Rocco A. Montone18Department of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiology S. Maria dei Battuti Hospital, AULSS 2 Veneto Conegliano TV ItalyHospital Clínic, Cardiovascular Clinic Institute, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) Barcelona SpainDivision of Cardiology Azienda Ospedaliero Universitaria Policlinico “G. Rodolico‐San Marco”, University of Catania Catania ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDivision of Cardiology University of Parma, Parma University Hospital Parma ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyGemelli Generator RWD Fondazione Policlinico Universitario A. Gemelli IRCCS Rome ItalyGemelli Generator RWD Fondazione Policlinico Universitario A. Gemelli IRCCS Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDivision of Cardiology University of Parma, Parma University Hospital Parma ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyDepartment of Cardiovascular and Pulmonary Sciences Catholic University of the Sacred Heart Rome ItalyBackground Intracoronary provocation testing with acetylcholine (ACh) is helpful to diagnose and risk‐stratify patients with ischemia with nonobstructed coronary arteries (NOCA) and myocardial infarction with NOCA. This study explored potential sex‐related disparities on the prognostic significance of ACh provocative testing. Methods Consecutive patients with ischemia with NOCA and those with myocardial infarction with NOCA who underwent ACh provocation testing were enrolled. The primary end point was the incidence of major adverse cardiovascular and cerebrovascular events at follow‐up. Co‐primary end points were angina recurrence and quality of life assessed by 12‐month Seattle Angina Questionnaire (SAQ) summary score. Results A total of 519 patients (mean age, 61.4±12.1 years; 275 [53.0%] women and 244 [47%] men) were enrolled: 346 (66.7%) with ischemia with NOCA and 173 (33.3%) with myocardial infarction with NOCA. A positive ACh test was observed in 274 (52.8%) patients, with a lower prevalence of epicardial spasm (82 [56.2%] versus 106 [82.8%]) and a higher prevalence of microvascular spasm (64 [43.8%] versus 22 [17.2%]) in women compared with men (P>0.001). After a median 22‐month follow‐up, major adverse cardiovascular and cerebrovascular events occurred in 53 (10.2%) patients, without significant sex differences (P>0.05). Men with a positive ACh test had a significantly higher rate of major adverse cardiovascular and cerebrovascular events (22 [17.2%] versus 5 [4.3%], P=0.002) compared with those with a negative test; no difference was observed in women (P>0.05) (P for interaction=0.003). Women with a positive test experienced a higher rate of angina recurrence (61 [41.8%] versus 32 [24.8%], P=0.005) and a lower SAQ summary score (82 [interquartile range, 72–90] versus 86 [interquartile range, 78–100], P<0.001) compared with those with a negative result; no difference was observed in men (P>0.05). Conclusions This study revealed the importance of recognizing sex‐specific differences in the prognostic value of ACh testing for proper management of coronary vasomotor disorders.https://www.ahajournals.org/doi/10.1161/JAHA.124.037942acetylcholineINOCAMINOCAprognosissex
spellingShingle Riccardo Rinaldi
Michele Russo
Giovanni Occhipinti
Claudio Laudani
Ilaria Torre
Michele Colucci
Filippo Luca Gurgoglione
Francesco Maria Animati
Jacopo Lenkowicz
Andrada Mihaela Tudor
Giovanna Liuzzo
Tommaso Sanna
Antonio Maria Leone
Giampaolo Niccoli
Gaetano A. Lanza
Carlo Trani
Francesco Burzotta
Filippo Crea
Rocco A. Montone
Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing
Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
acetylcholine
INOCA
MINOCA
prognosis
sex
title Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing
title_full Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing
title_fullStr Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing
title_full_unstemmed Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing
title_short Sex‐Related Differences in the Prognostic Role of Acetylcholine Provocation Testing
title_sort sex related differences in the prognostic role of acetylcholine provocation testing
topic acetylcholine
INOCA
MINOCA
prognosis
sex
url https://www.ahajournals.org/doi/10.1161/JAHA.124.037942
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