Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis
Introduction: Systematic evaluation of the diagnostic value of next generation sequencing (NGS) in sepsis etiology. Methodology: We conducted a systematic search on four databases (Web of Science, Cochrane, PubMed, and Embase) and compiled diagnostic experiments using NGS to evaluate sepsis etiol...
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The Journal of Infection in Developing Countries
2023-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/18235 |
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| author | Dong Li Yinghao Yang Ying Xie Jianrong Wang Wensheng Xu Junxue Wang Xiaofeng Hang |
| author_facet | Dong Li Yinghao Yang Ying Xie Jianrong Wang Wensheng Xu Junxue Wang Xiaofeng Hang |
| author_sort | Dong Li |
| collection | DOAJ |
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Introduction: Systematic evaluation of the diagnostic value of next generation sequencing (NGS) in sepsis etiology.
Methodology: We conducted a systematic search on four databases (Web of Science, Cochrane, PubMed, and Embase) and compiled diagnostic experiments using NGS to evaluate sepsis etiology. Two researchers conducted research and obtained data independently.
Results: Nine documents were included comprising 747 patients, 988 blood samples, 175 bronchoalveolar lavage fluid (BALF) samples, 16 cerebrospinal fluid samples, and one urine sample. The combined sensitivity of each study was 0.89 (95% CI: 0.82-0.95). The combined specificity was 0.40 (95% CI: 0.25-0.55). The combined positive likelihood ratio was 1.51 (95% CI: 1.18-1.98). The combined negative likelihood ratio was 0.28 (95% CI: 0.11-0.48). The diagnostic odds ratio (DOR) was 6.38 (95% CI: 2.53-15.32) and the area under the curve (AUC) was 0.84, (95% CI: 0.62-0.94).
Conclusions: Based on the data we collected, we found that compared with the blood culture technology, NGS has the advantages of high sensitivity and wide detection range, but its specificity was low. Further study is needed to confirm the value of NGS in the etiological diagnosis of patients with sepsis.
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| format | Article |
| id | doaj-art-00a24464cc264f9ab8644818ffdb67ae |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-00a24464cc264f9ab8644818ffdb67ae2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-12-01171210.3855/jidc.18235Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysisDong Li0Yinghao Yang1Ying Xie2Jianrong Wang3Wensheng Xu4Junxue Wang5Xiaofeng Hang6Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, ChinaDepartment of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, ChinaDepartment of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, ChinaDepartment of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, ChinaDepartment of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, ChinaDepartment of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, ChinaDepartment of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China Introduction: Systematic evaluation of the diagnostic value of next generation sequencing (NGS) in sepsis etiology. Methodology: We conducted a systematic search on four databases (Web of Science, Cochrane, PubMed, and Embase) and compiled diagnostic experiments using NGS to evaluate sepsis etiology. Two researchers conducted research and obtained data independently. Results: Nine documents were included comprising 747 patients, 988 blood samples, 175 bronchoalveolar lavage fluid (BALF) samples, 16 cerebrospinal fluid samples, and one urine sample. The combined sensitivity of each study was 0.89 (95% CI: 0.82-0.95). The combined specificity was 0.40 (95% CI: 0.25-0.55). The combined positive likelihood ratio was 1.51 (95% CI: 1.18-1.98). The combined negative likelihood ratio was 0.28 (95% CI: 0.11-0.48). The diagnostic odds ratio (DOR) was 6.38 (95% CI: 2.53-15.32) and the area under the curve (AUC) was 0.84, (95% CI: 0.62-0.94). Conclusions: Based on the data we collected, we found that compared with the blood culture technology, NGS has the advantages of high sensitivity and wide detection range, but its specificity was low. Further study is needed to confirm the value of NGS in the etiological diagnosis of patients with sepsis. https://jidc.org/index.php/journal/article/view/18235Sepsisbloodstream infectionnext generation sequencingHigh-Throughput Nucleotide Sequencing |
| spellingShingle | Dong Li Yinghao Yang Ying Xie Jianrong Wang Wensheng Xu Junxue Wang Xiaofeng Hang Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis Journal of Infection in Developing Countries Sepsis bloodstream infection next generation sequencing High-Throughput Nucleotide Sequencing |
| title | Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis |
| title_full | Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis |
| title_fullStr | Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis |
| title_full_unstemmed | Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis |
| title_short | Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis |
| title_sort | evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology a systematic review and meta analysis |
| topic | Sepsis bloodstream infection next generation sequencing High-Throughput Nucleotide Sequencing |
| url | https://jidc.org/index.php/journal/article/view/18235 |
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