A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria
We have determined the nucleotide sequence of the mim3-1 mitochondrial ribosomal suppressor, acting on ochre mitochondrial mutations and one frameshift mutation in Saccharomyces cerevisiae. The 15s rRNA suppressor gene contains a G633 to C transversion. Yeast mitochondrial G633 corr...
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Shared Science Publishers OG
2015-08-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/a-single-mutation-in-the-15s-rrna-gene-confers-non-sense-suppressor-activity-and-interacts-with-mrf1-the-release-factor-in-yeast-mitochondria/ |
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| author | Ali Gargouri Catherine Macadré Jaga Lazowska |
| author_facet | Ali Gargouri Catherine Macadré Jaga Lazowska |
| author_sort | Ali Gargouri |
| collection | DOAJ |
| description | We have determined the nucleotide sequence of the mim3-1 mitochondrial ribosomal suppressor, acting on ochre mitochondrial mutations and one frameshift mutation in Saccharomyces cerevisiae. The 15s rRNA suppressor gene contains a G633 to C transversion. Yeast mitochondrial G633 corresponds to G517 of the E.coli 15S rRNA, which is occupied by an invariant G in all known small rRNA sequences. Interestingly, this mutation has occurred at the same position as the known MSU1 mitochondrial suppressor which changes G633 to A. The suppressor mutation lies in a highly conserved region of the rRNA, known in E.coli as the 530-loop, interacting with the S4, S5 and S12 ribosomal proteins. We also show an interesting interaction between the mitochondrial mim3-1 and the nuclear nam3-1 suppressors, both of which have the same action spectrum on mitochondrial mutations: nam3-1 abolishes the suppressor effect when present with mim3-1 in the same haploid cell. We discuss these results in the light of the nature of Nam3, identified by [1] as the yeast mitochondrial translation release factor. A hypothetical mechanism of suppression by "ribosome shifting" is also discussed in view of the nature of mutations suppressed and not suppressed. |
| format | Article |
| id | doaj-art-00a0221c555c4cd29f572b45d89731ab |
| institution | OA Journals |
| issn | 2311-2638 |
| language | English |
| publishDate | 2015-08-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-00a0221c555c4cd29f572b45d89731ab2025-08-20T02:04:33ZengShared Science Publishers OGMicrobial Cell2311-26382015-08-012934335210.15698/mic2015.09.223123455678A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondriaAli Gargouri0Catherine Macadré1Jaga Lazowska2Centre de Génétique Moléculaire, Laboratoire propre du C.N.R.S associé à l'Université Pierre et Marie Curie. CNRS F-91198 Gif-sur-Yvette cedex, France.Centre de Génétique Moléculaire, Laboratoire propre du C.N.R.S associé à l'Université Pierre et Marie Curie. CNRS F-91198 Gif-sur-Yvette cedex, France.Centre de Génétique Moléculaire, Laboratoire propre du C.N.R.S associé à l'Université Pierre et Marie Curie. CNRS F-91198 Gif-sur-Yvette cedex, France.We have determined the nucleotide sequence of the mim3-1 mitochondrial ribosomal suppressor, acting on ochre mitochondrial mutations and one frameshift mutation in Saccharomyces cerevisiae. The 15s rRNA suppressor gene contains a G633 to C transversion. Yeast mitochondrial G633 corresponds to G517 of the E.coli 15S rRNA, which is occupied by an invariant G in all known small rRNA sequences. Interestingly, this mutation has occurred at the same position as the known MSU1 mitochondrial suppressor which changes G633 to A. The suppressor mutation lies in a highly conserved region of the rRNA, known in E.coli as the 530-loop, interacting with the S4, S5 and S12 ribosomal proteins. We also show an interesting interaction between the mitochondrial mim3-1 and the nuclear nam3-1 suppressors, both of which have the same action spectrum on mitochondrial mutations: nam3-1 abolishes the suppressor effect when present with mim3-1 in the same haploid cell. We discuss these results in the light of the nature of Nam3, identified by [1] as the yeast mitochondrial translation release factor. A hypothetical mechanism of suppression by "ribosome shifting" is also discussed in view of the nature of mutations suppressed and not suppressed.http://microbialcell.com/researcharticles/a-single-mutation-in-the-15s-rrna-gene-confers-non-sense-suppressor-activity-and-interacts-with-mrf1-the-release-factor-in-yeast-mitochondria/yeastinformational suppressors15S rRNAnonsenseframe shift |
| spellingShingle | Ali Gargouri Catherine Macadré Jaga Lazowska A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria Microbial Cell yeast informational suppressors 15S rRNA nonsense frame shift |
| title | A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria |
| title_full | A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria |
| title_fullStr | A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria |
| title_full_unstemmed | A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria |
| title_short | A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria |
| title_sort | single mutation in the 15s rrna gene confers nonsense suppressor activity and interacts with mrf1 the release factor in yeast mitochondria |
| topic | yeast informational suppressors 15S rRNA nonsense frame shift |
| url | http://microbialcell.com/researcharticles/a-single-mutation-in-the-15s-rrna-gene-confers-non-sense-suppressor-activity-and-interacts-with-mrf1-the-release-factor-in-yeast-mitochondria/ |
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