Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies
Abstract To develop economically viable and environmentally benign methodologies for organic reactions and reveal the practical utility of transitional natural compounds and their derivatives. In addition, a new research method to conduct docking studies against nuclear factors sheds light on the th...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-05-01
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| Series: | Applied Biological Chemistry |
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| Online Access: | https://doi.org/10.1186/s13765-025-00982-2 |
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| author | Mohamed A. M. El-Tabakh Ahmed Z. I. Shehata Ahmed M. Sadek Salem S. Salem Ahmed A. Elmehdawy Mahmoud Nazih Gamal M. Omar Ahmed A. Abo Elsoud Ahmed N. G. Abdel-Aziz Sozan Eid El-Abeid Heba F. Abd-Elkhalek Omnia M. Arief |
| author_facet | Mohamed A. M. El-Tabakh Ahmed Z. I. Shehata Ahmed M. Sadek Salem S. Salem Ahmed A. Elmehdawy Mahmoud Nazih Gamal M. Omar Ahmed A. Abo Elsoud Ahmed N. G. Abdel-Aziz Sozan Eid El-Abeid Heba F. Abd-Elkhalek Omnia M. Arief |
| author_sort | Mohamed A. M. El-Tabakh |
| collection | DOAJ |
| description | Abstract To develop economically viable and environmentally benign methodologies for organic reactions and reveal the practical utility of transitional natural compounds and their derivatives. In addition, a new research method to conduct docking studies against nuclear factors sheds light on the theoretical mechanism of action of Phlomis aurea extracts as antioxidant, antimicrobial, anticancer, and repellent. The pharmacological potential of Phlomis aurea is investigated in this research by analysing its aqueous and petroleum ether extracts. So, to evaluate antioxidant activity, the DPPH scavenging test was used and compared against ascorbic acid; aqueous extract showed noteworthy activity. Both extracts demonstrated noteworthy efficacy against various pathogens, such as Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. The anti-cancer activity was also assessed using in-vitro assay on a standard cell line (Wi38) and two cancer cell lines (MDA and HepG2). The sensitivity of starving female An. pharoensis to the studied extracts was higher than that of Cx. pipiens, suggesting that these extracts may have potential applications in vector control. Docking study against nuclear factor erythroid 2–related factor 2 (Nrf2) (PDB ID: 3wn7), topoisomerase IV (PDB ID: 7lhz), COX protein (PDB ID: 6y3c), and Odorant Binding Protein 7 (OBP7) (PDB ID: 3r1o), to shed light on the theoretical mechanism expected as anti-oxidant, anti-microbial, anti-cancer and repellent effects against mosquitoes respectively, for galic acid as most significantly quantifying compounds on both extracts; highlighting the predicted mechanism of the proposed in-vitro assay, and confirming the present result. |
| format | Article |
| id | doaj-art-009bb88afac14c868ec7e35ad154645a |
| institution | Kabale University |
| issn | 2468-0842 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj-art-009bb88afac14c868ec7e35ad154645a2025-08-20T03:48:15ZengSpringerOpenApplied Biological Chemistry2468-08422025-05-0168111610.1186/s13765-025-00982-2Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studiesMohamed A. M. El-Tabakh0Ahmed Z. I. Shehata1Ahmed M. Sadek2Salem S. Salem3Ahmed A. Elmehdawy4Mahmoud Nazih5Gamal M. Omar6Ahmed A. Abo Elsoud7Ahmed N. G. Abdel-Aziz8Sozan Eid El-Abeid9Heba F. Abd-Elkhalek10Omnia M. Arief11Zoology Department, Faculty of Science, Al-Azhar UniversityZoology Department, Faculty of Science, Al-Azhar UniversityBotany and Microbiology Department, Faculty of Science, Al-Azhar UniversityBotany and Microbiology Department, Faculty of Science, Al-Azhar UniversityZoology Department, Faculty of Science, Al-Azhar UniversityDepartment of Clinical Pharmacy, Faculty of Pharmacy, Menoufia UniversityZoology Department, Faculty of Science, Al-Azhar UniversityZoology Department, Faculty of Science, Al-Azhar UniversityConsultant Medical Entomologist at the Public Health Department, Jazan MunicipalityNanotechnology & Advanced Nano-Materials Laboratory (NANML), Mycology and Disease Survey Research Department, Plant Pathology Research Institute, Agricultural Research CenterEntomology Department, Faculty of Science, Benha UniversityBotany and Microbiology Department, Faculty of Science, Benha UniversityAbstract To develop economically viable and environmentally benign methodologies for organic reactions and reveal the practical utility of transitional natural compounds and their derivatives. In addition, a new research method to conduct docking studies against nuclear factors sheds light on the theoretical mechanism of action of Phlomis aurea extracts as antioxidant, antimicrobial, anticancer, and repellent. The pharmacological potential of Phlomis aurea is investigated in this research by analysing its aqueous and petroleum ether extracts. So, to evaluate antioxidant activity, the DPPH scavenging test was used and compared against ascorbic acid; aqueous extract showed noteworthy activity. Both extracts demonstrated noteworthy efficacy against various pathogens, such as Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. The anti-cancer activity was also assessed using in-vitro assay on a standard cell line (Wi38) and two cancer cell lines (MDA and HepG2). The sensitivity of starving female An. pharoensis to the studied extracts was higher than that of Cx. pipiens, suggesting that these extracts may have potential applications in vector control. Docking study against nuclear factor erythroid 2–related factor 2 (Nrf2) (PDB ID: 3wn7), topoisomerase IV (PDB ID: 7lhz), COX protein (PDB ID: 6y3c), and Odorant Binding Protein 7 (OBP7) (PDB ID: 3r1o), to shed light on the theoretical mechanism expected as anti-oxidant, anti-microbial, anti-cancer and repellent effects against mosquitoes respectively, for galic acid as most significantly quantifying compounds on both extracts; highlighting the predicted mechanism of the proposed in-vitro assay, and confirming the present result.https://doi.org/10.1186/s13765-025-00982-2Phlomis aureaCulex pipiensAnopheles pharoensisAntibacterial activityCytotoxicity |
| spellingShingle | Mohamed A. M. El-Tabakh Ahmed Z. I. Shehata Ahmed M. Sadek Salem S. Salem Ahmed A. Elmehdawy Mahmoud Nazih Gamal M. Omar Ahmed A. Abo Elsoud Ahmed N. G. Abdel-Aziz Sozan Eid El-Abeid Heba F. Abd-Elkhalek Omnia M. Arief Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies Applied Biological Chemistry Phlomis aurea Culex pipiens Anopheles pharoensis Antibacterial activity Cytotoxicity |
| title | Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies |
| title_full | Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies |
| title_fullStr | Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies |
| title_full_unstemmed | Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies |
| title_short | Multifunctional properties of Phlomis aurea extracts: In-vitro antioxidant, antimicrobial, anticancer, potent repellency against two mosquito vectors and molecular docking studies |
| title_sort | multifunctional properties of phlomis aurea extracts in vitro antioxidant antimicrobial anticancer potent repellency against two mosquito vectors and molecular docking studies |
| topic | Phlomis aurea Culex pipiens Anopheles pharoensis Antibacterial activity Cytotoxicity |
| url | https://doi.org/10.1186/s13765-025-00982-2 |
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