High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand functionalization effects

High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand functionalization effects

Abstract Background Cluster of differentiation 95 (CD95/Fas/Apo1) as part of the Tumor-necrosis factor (TNF) receptor family is a prototypic trigger of the ‘extrinsic’ apoptotic pathway and its activation by the trimeric ligand CD95L is of high interest. However, CD95L, when presented in solution, e...

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Bibliographic Details
Main Authors: Xiaoyue Shang, Nina Bartels, Johann Moritz Weck, Sabine Suppmann, Jérôme Basquin, Gajen Thaventhiran, Amelie Heuer-Jungemann, Cornelia Monzel
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Biotechnology
Subjects:
CD95 ligand
Isoleucine zipper
Mammalian expression
Cell factory
Site-specific modification
DNA origami
Online Access:https://doi.org/10.1186/s12896-025-00986-2
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