Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats
Objectives. To estimate the impact of ellagic acid (EA) towards healing tooth socket in diabetic animals, after tooth extraction. Methods. Twenty-four Sprague Dawley male rats weighing 250–300 g were selected for this study. All animals were intraperitoneally injected with 45 mg/kg (b.w.) of freshly...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/908098 |
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| author | Mazen M. Jamil Al-Obaidi Fouad Hussain Al-Bayaty Rami Al Batran Jamal Hussaini Goot Heah Khor |
| author_facet | Mazen M. Jamil Al-Obaidi Fouad Hussain Al-Bayaty Rami Al Batran Jamal Hussaini Goot Heah Khor |
| author_sort | Mazen M. Jamil Al-Obaidi |
| collection | DOAJ |
| description | Objectives. To estimate the impact of ellagic acid (EA) towards healing tooth socket in diabetic animals, after tooth extraction. Methods. Twenty-four Sprague Dawley male rats weighing 250–300 g were selected for this study. All animals were intraperitoneally injected with 45 mg/kg (b.w.) of freshly prepared streptozotocin (STZ), to induce diabetic mellitus. Then, the animals were anesthetized, and the upper left central incisor was extracted and the whole extracted sockets were filled with Rosuvastatin (RSV). The rats were separated into three groups, comprising 8 rats each. The first group was considered as normal control group and orally treated with normal saline. The second group was regarded as diabetic control group and orally treated with normal saline, whereas the third group comprised diabetic rats, administrated with EA (50 mg/kg) orally. The maxilla tissue stained by eosin and hematoxylin (H&E) was used for histological examinations and immunohistochemical technique. Fibroblast growth factor (FGF-2) and alkaline phosphatase (ALP) were used to evaluate the healing process in the extracted tooth socket by immunohistochemistry test. Results. The reactions of immunohistochemistry for FGF-2 and ALP presented stronger expression, predominantly in EA treated diabetic rat, than the untreated diabetic rat. Conclusion. These findings suggest that the administration of EA combined with RSV may have accelerated the healing process of the tooth socket of diabetic rats, after tooth extraction. |
| format | Article |
| id | doaj-art-008cb963146d466fbabb49b00f2963f5 |
| institution | DOAJ |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-008cb963146d466fbabb49b00f2963f52025-08-20T03:22:31ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/908098908098Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic RatsMazen M. Jamil Al-Obaidi0Fouad Hussain Al-Bayaty1Rami Al Batran2Jamal Hussaini3Goot Heah Khor4Centre of Studies for Periodontology, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, MalaysiaCentre of Studies for Periodontology, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, MalaysiaCentre of Studies for Periodontology, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, MalaysiaFaculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor, MalaysiaCenter of Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, MalaysiaObjectives. To estimate the impact of ellagic acid (EA) towards healing tooth socket in diabetic animals, after tooth extraction. Methods. Twenty-four Sprague Dawley male rats weighing 250–300 g were selected for this study. All animals were intraperitoneally injected with 45 mg/kg (b.w.) of freshly prepared streptozotocin (STZ), to induce diabetic mellitus. Then, the animals were anesthetized, and the upper left central incisor was extracted and the whole extracted sockets were filled with Rosuvastatin (RSV). The rats were separated into three groups, comprising 8 rats each. The first group was considered as normal control group and orally treated with normal saline. The second group was regarded as diabetic control group and orally treated with normal saline, whereas the third group comprised diabetic rats, administrated with EA (50 mg/kg) orally. The maxilla tissue stained by eosin and hematoxylin (H&E) was used for histological examinations and immunohistochemical technique. Fibroblast growth factor (FGF-2) and alkaline phosphatase (ALP) were used to evaluate the healing process in the extracted tooth socket by immunohistochemistry test. Results. The reactions of immunohistochemistry for FGF-2 and ALP presented stronger expression, predominantly in EA treated diabetic rat, than the untreated diabetic rat. Conclusion. These findings suggest that the administration of EA combined with RSV may have accelerated the healing process of the tooth socket of diabetic rats, after tooth extraction.http://dx.doi.org/10.1155/2014/908098 |
| spellingShingle | Mazen M. Jamil Al-Obaidi Fouad Hussain Al-Bayaty Rami Al Batran Jamal Hussaini Goot Heah Khor Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats The Scientific World Journal |
| title | Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats |
| title_full | Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats |
| title_fullStr | Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats |
| title_full_unstemmed | Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats |
| title_short | Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats |
| title_sort | impact of ellagic acid in bone formation after tooth extraction an experimental study on diabetic rats |
| url | http://dx.doi.org/10.1155/2014/908098 |
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