Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose
Objective To investigate the effect and possible mechanisms of angiotensin Ⅱ type1 receptor(AT1 R) on injury of renal tubular epithelial cells induced by high glucose. Methods The expression of AT1 R,AdipoR1,NF-κB,MCP-1,MIP-1 α,α-SMA,FN were detected by using western blot. The colocalization of AT1...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2018-01-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57914718&Fpath=home&index=0 |
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| Summary: | Objective To investigate the effect and possible mechanisms of angiotensin Ⅱ type1 receptor(AT1 R) on injury of renal tubular epithelial cells induced by high glucose. Methods The expression of AT1 R,AdipoR1,NF-κB,MCP-1,MIP-1 α,α-SMA,FN were detected by using western blot. The colocalization of AT1 R and AdipoR1 was detected by confocal microscopy. The dimerization of AT1 R and AdipoR1 was detected by immunoprecipitation. Results Compared with control group,the expression of AdipoR1 had no significant change and the expression of AT1 R was increased in rat renal tubular epithelial cells induced by high glucose. The expression of inflammatory factors as NF-κB,MCP-1 and MIP-1 α was increased in rat renal tubular epithelial cells stimulated with high glucose.Compared with control group,the expression of α-SMA and FN was increased in rat renal tubular epithelial cells stimulated with high glucose. The dimerization of AT1 R and AdipoR1 was formed in the rat renal tubular epithelial cells. And the AT1 R-adipoR1 dimerization was increased in the rat renal tubular epithelial cells induced by high glucose. Knockdown of AT1 R by siRNA inhibited the demerization of AT1 R-adipoR1,reduced the expression of inflammatory factors as NF-κB,MCP-1 and MIP-1 α,decreased the expression of α-SMA and FN in rat renal tubular epithelial cells induced by high glucose.Conclusions AT1 R promotes the inflammation and fibrosis of renal tubular epithelial cells induced by high glucose through dimerization of AT1 R-adipoR1. |
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| ISSN: | 1671-2390 |