Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize
Iron (Fe) deficiency is a pervasive agricultural concern on a global scale. Intercropping plays a pivotal role in activating soil nutrient cycling and crop nutrient uptake and utilization. This study integrates plant physiology, soil physicochemical determination, high-throughput sequencing, and met...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/2/286 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849722582019866624 |
|---|---|
| author | Wuyu Liu Guoqing Wang Shiming Wen Yiwen Zhao Yuxin Ding Baihui Yao Zhelin Wang Duntao Shu Gehong Wei Juan Chen Zhouping Shangguan |
| author_facet | Wuyu Liu Guoqing Wang Shiming Wen Yiwen Zhao Yuxin Ding Baihui Yao Zhelin Wang Duntao Shu Gehong Wei Juan Chen Zhouping Shangguan |
| author_sort | Wuyu Liu |
| collection | DOAJ |
| description | Iron (Fe) deficiency is a pervasive agricultural concern on a global scale. Intercropping plays a pivotal role in activating soil nutrient cycling and crop nutrient uptake and utilization. This study integrates plant physiology, soil physicochemical determination, high-throughput sequencing, and metabolomics techniques to conduct pot experiments using field-collected soils with soybean and maize plants. This study aims to investigate the mechanisms through which microorganisms in a soybean–maize intercropping system regulate Fe deficiency adaptation. The results revealed that intercropping enhances the resilience of soybean and maize in Fe-deficient environments, facilitates nutrient absorption by plants, and enriches soil nutrient content. Moreover, intercropping fostered more intricate microbial interactions in comparison to monocropping. The dominant microorganisms in the rhizosphere of intercropped soybean and maize included genera <i>Microbacterium</i>, <i>Sphingomonas</i>, <i>Shinella,</i> and <i>Rhizobium</i>. <i>Microbacterium</i>, <i>Sphingomonas</i>, <i>Shinella,</i> and <i>Rhizobium</i> have the potential to produce Fe chelators or enhance plant Fe absorption. Additionally, intercropping notably modified the composition of root exudates derived from soybean and maize. The soybean and maize rhizosphere exhibited significant enrichment with oleamide, coumestrol, glycitein, and daidzein. Coumestrol may have an effect of promoting Fe absorption, and it is significantly positively correlated with the genus <i>Nakamurella</i> in the maize rhizosphere and the genus <i>Pirellula</i> in the soybean rhizosphere. Consequently, these findings suggested that the rhizosphere of intercropped soybean and maize significantly enriches specific microbial communities and root exudates, thereby enhancing microecosystem stability and improving plant tolerance to Fe deficiency. |
| format | Article |
| id | doaj-art-007db5ac070f477abe6da705e749c152 |
| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-007db5ac070f477abe6da705e749c1522025-08-20T03:11:18ZengMDPI AGAgronomy2073-43952025-01-0115228610.3390/agronomy15020286Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and MaizeWuyu Liu0Guoqing Wang1Shiming Wen2Yiwen Zhao3Yuxin Ding4Baihui Yao5Zhelin Wang6Duntao Shu7Gehong Wei8Juan Chen9Zhouping Shangguan10State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Xianyang 712100, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Xianyang 712100, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Science, Northwest A&F University, Xianyang 712100, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Xianyang 712100, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Xianyang 712100, ChinaCollege of Natural Resources and Environment, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Science, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Science, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Science, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Xianyang 712100, ChinaIron (Fe) deficiency is a pervasive agricultural concern on a global scale. Intercropping plays a pivotal role in activating soil nutrient cycling and crop nutrient uptake and utilization. This study integrates plant physiology, soil physicochemical determination, high-throughput sequencing, and metabolomics techniques to conduct pot experiments using field-collected soils with soybean and maize plants. This study aims to investigate the mechanisms through which microorganisms in a soybean–maize intercropping system regulate Fe deficiency adaptation. The results revealed that intercropping enhances the resilience of soybean and maize in Fe-deficient environments, facilitates nutrient absorption by plants, and enriches soil nutrient content. Moreover, intercropping fostered more intricate microbial interactions in comparison to monocropping. The dominant microorganisms in the rhizosphere of intercropped soybean and maize included genera <i>Microbacterium</i>, <i>Sphingomonas</i>, <i>Shinella,</i> and <i>Rhizobium</i>. <i>Microbacterium</i>, <i>Sphingomonas</i>, <i>Shinella,</i> and <i>Rhizobium</i> have the potential to produce Fe chelators or enhance plant Fe absorption. Additionally, intercropping notably modified the composition of root exudates derived from soybean and maize. The soybean and maize rhizosphere exhibited significant enrichment with oleamide, coumestrol, glycitein, and daidzein. Coumestrol may have an effect of promoting Fe absorption, and it is significantly positively correlated with the genus <i>Nakamurella</i> in the maize rhizosphere and the genus <i>Pirellula</i> in the soybean rhizosphere. Consequently, these findings suggested that the rhizosphere of intercropped soybean and maize significantly enriches specific microbial communities and root exudates, thereby enhancing microecosystem stability and improving plant tolerance to Fe deficiency.https://www.mdpi.com/2073-4395/15/2/286intercroppingsoybeanmaizeiron deficiencymicrobial communityroot exudates |
| spellingShingle | Wuyu Liu Guoqing Wang Shiming Wen Yiwen Zhao Yuxin Ding Baihui Yao Zhelin Wang Duntao Shu Gehong Wei Juan Chen Zhouping Shangguan Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize Agronomy intercropping soybean maize iron deficiency microbial community root exudates |
| title | Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize |
| title_full | Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize |
| title_fullStr | Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize |
| title_full_unstemmed | Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize |
| title_short | Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize |
| title_sort | microbiome mediated mechanisms regulating adaptability to iron deficiency in the intercropping system of soybean and maize |
| topic | intercropping soybean maize iron deficiency microbial community root exudates |
| url | https://www.mdpi.com/2073-4395/15/2/286 |
| work_keys_str_mv | AT wuyuliu microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT guoqingwang microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT shimingwen microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT yiwenzhao microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT yuxinding microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT baihuiyao microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT zhelinwang microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT duntaoshu microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT gehongwei microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT juanchen microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize AT zhoupingshangguan microbiomemediatedmechanismsregulatingadaptabilitytoirondeficiencyintheintercroppingsystemofsoybeanandmaize |