Bacterial Coaggregation in Bacteria Isolated from Dental Unit Waterlines
Background: To understand the process of biofilm formation in dental unit waterlines (DUWLs), it is necessary to assess the of bacteria that constitute DUWL biofilms. This study assessed the coaggregation of bacterial species isolated from DUWL biofilms and other related mechanisms. Methods: Coag...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
The Korean Society of Dental Hygiene Science
2024-12-01
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| Series: | 치위생과학회지 |
| Subjects: | |
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| Summary: | Background: To understand the process of biofilm formation in dental unit waterlines (DUWLs), it is necessary to assess the of
bacteria that constitute DUWL biofilms. This study assessed the coaggregation of bacterial species isolated from DUWL biofilms
and other related mechanisms.
Methods: Coaggregation was macroscopically evaluated. Cultures of the DUWLs isolates were adjusted to the same concentration
(optical density [OD]600=1.5). The isolates were mixed in pairs and incubated for 1 hour at room temperature. Subsequently, the
coaggregation values were scored. Some coaggregation pairs were observed through fluorescence microscope after staining
with SYTOⓇ 9 and propidium iodide. To assess their coaggregation properties, inhibition of coaggregation was performed by
adding sugars and amino acids to the coaggregation pairs and the coaggregation values were scored again post-addition.
Results: Coaggregation was observed in 360 out of 406 coaggregation pairs, whose coaggregation scores of ranged from one to
three points. Five pairs (1.4%) had a coaggregation score of 3 points. Among these five pairs, mannose most frequently inhibited
coaggregation. Among the isolates, Cupriavidus pauculus HY12 and Beijerinckia derxii subsp. venezuelae HY7 had the highest
number of coaggregation pairs, with scores of 2 and 3.
Conclusion: Cupriavidus pauculus HY12 and B. derxii subsp. venezuelae HY7 form many coaggregation pairs and may play
important roles in biofilm maturation. The results of this study can be used to propose methods to inhibit the formation of DUWL
biofilms by inhibiting coaggregation. |
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| ISSN: | 2233-7679 |