Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow.
Continuous monitoring of the present genetic status is essential to preserve the genetic resource of wild populations. In this study, we sequenced regional Pacific abalone Haliotis discus samples from three different locations around the Korean peninsula to assess population structure, utilizing Gen...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0247815&type=printable |
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| author | Bo-Hye Nam Hyaekang Kim Donghyeok Seol Heebal Kim Eun Soo Noh Eun Mi Kim Jae Koo Noh Young-Ok Kim Jung Youn Park Woori Kwak |
| author_facet | Bo-Hye Nam Hyaekang Kim Donghyeok Seol Heebal Kim Eun Soo Noh Eun Mi Kim Jae Koo Noh Young-Ok Kim Jung Youn Park Woori Kwak |
| author_sort | Bo-Hye Nam |
| collection | DOAJ |
| description | Continuous monitoring of the present genetic status is essential to preserve the genetic resource of wild populations. In this study, we sequenced regional Pacific abalone Haliotis discus samples from three different locations around the Korean peninsula to assess population structure, utilizing Genotyping-by-Sequencing (GBS) method. Using PstI enzyme for genome reduction, we demonstrated the resultant library represented the whole genome region with even spacing, and as a result 16,603 single nucleotide variants (SNVs) were produced. Genetic diversity and population structure were investigated using several methods, and a strong genetic heterogeneity was observed in the Korean abalone populations. Additionally, by comparison of the variant sets among population groups, we were able to discover 26 Korean abalone population-specific SNVs, potentially associated with phenotype differences. This is the first study demonstrating the feasibility of GBS for population genetic study on H. discus. Our results will provide valuable data for the genetic conservation and management of wild abalone populations in Korea and help future GBS studies on the marine mollusks. |
| format | Article |
| id | doaj-art-0075fb5a6eab4887a7e69bca3a3c0983 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-0075fb5a6eab4887a7e69bca3a3c09832025-08-20T02:54:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01164e024781510.1371/journal.pone.0247815Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow.Bo-Hye NamHyaekang KimDonghyeok SeolHeebal KimEun Soo NohEun Mi KimJae Koo NohYoung-Ok KimJung Youn ParkWoori KwakContinuous monitoring of the present genetic status is essential to preserve the genetic resource of wild populations. In this study, we sequenced regional Pacific abalone Haliotis discus samples from three different locations around the Korean peninsula to assess population structure, utilizing Genotyping-by-Sequencing (GBS) method. Using PstI enzyme for genome reduction, we demonstrated the resultant library represented the whole genome region with even spacing, and as a result 16,603 single nucleotide variants (SNVs) were produced. Genetic diversity and population structure were investigated using several methods, and a strong genetic heterogeneity was observed in the Korean abalone populations. Additionally, by comparison of the variant sets among population groups, we were able to discover 26 Korean abalone population-specific SNVs, potentially associated with phenotype differences. This is the first study demonstrating the feasibility of GBS for population genetic study on H. discus. Our results will provide valuable data for the genetic conservation and management of wild abalone populations in Korea and help future GBS studies on the marine mollusks.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0247815&type=printable |
| spellingShingle | Bo-Hye Nam Hyaekang Kim Donghyeok Seol Heebal Kim Eun Soo Noh Eun Mi Kim Jae Koo Noh Young-Ok Kim Jung Youn Park Woori Kwak Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow. PLoS ONE |
| title | Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow. |
| title_full | Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow. |
| title_fullStr | Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow. |
| title_full_unstemmed | Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow. |
| title_short | Genotyping-by-Sequencing of the regional Pacific abalone (Haliotis discus) genomes reveals population structures and patterns of gene flow. |
| title_sort | genotyping by sequencing of the regional pacific abalone haliotis discus genomes reveals population structures and patterns of gene flow |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0247815&type=printable |
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