LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis
Background. Gastric cancer (GC) is the most common malignant tumor and ranks third in the world. LncRNA H19 (H19), one of the members of lncRNA, is overexpressed in various tumors. However, many undetermined molecular mechanisms by which H19 promotes GC progression still need to be further investiga...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2024/6217134 |
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| author | Xin Zhang Ge Wang Xiaoru Li Yanqing Liu Xue Wu Yazhe Zhou Jie Liu Haiying Wang Rui Jiao Ying Chen Qiang Wang |
| author_facet | Xin Zhang Ge Wang Xiaoru Li Yanqing Liu Xue Wu Yazhe Zhou Jie Liu Haiying Wang Rui Jiao Ying Chen Qiang Wang |
| author_sort | Xin Zhang |
| collection | DOAJ |
| description | Background. Gastric cancer (GC) is the most common malignant tumor and ranks third in the world. LncRNA H19 (H19), one of the members of lncRNA, is overexpressed in various tumors. However, many undetermined molecular mechanisms by which H19 promotes GC progression still need to be further investigated. Methodology. A series of experiments was used to confirm the undetermined molecular mechanism including wound healing and transwell assays. Key Results. In this study, a significant upregulation of H19 expression was detected in GC cells and tissues. The poor overall survival was observed in GC patient with high H19 expression. Overexpression of H19 promoted the migration of GC cells, while knockdown of H19 significantly inhibited cell migration. Moreover, miR-148a-3p had a certain negative correlation with H19. Luciferase reporter assay confirmed that H19 could directly bind to miR-148a-3p. As expected, miR-148a mimics inhibited cell migration and invasion induced by H19 overexpression. The above findings proved that H19 functions as a miRNA sponge and verified that miR-148a-3p is the H19-associated miRNA in GC. We also confirmed that SOX-12 expression was upregulated in GC patient’s samples. SOX-12 expression was positively correlated with expression of H19 and was able to directly bind to miR-148a-3p. Importantly, in vitro wound healing assay showed that knockout of SOX-12 could reverse the promoting effect of H19 overexpression on cell migration. Conclusion. In conclusion, H19 has certain application value in the diagnosis and prognosis of GC. Specifically, H19 accelerates GCs to migration and metastasis by miR-138a-3p/SOX-12 axis. |
| format | Article |
| id | doaj-art-006262030857477893606d231fbfdffa |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-006262030857477893606d231fbfdffa2025-01-02T22:42:25ZengWileyAnalytical Cellular Pathology2210-71852024-01-01202410.1155/2024/6217134LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 AxisXin Zhang0Ge Wang1Xiaoru Li2Yanqing Liu3Xue Wu4Yazhe Zhou5Jie Liu6Haiying Wang7Rui Jiao8Ying Chen9Qiang Wang10Department of OrthopedicsDepartment of Cardiovascular SurgeryKey Laboratory of Resource Biology and Biotechnology in Western ChinaKey Laboratory of Resource Biology and Biotechnology in Western ChinaKey Laboratory of Resource Biology and Biotechnology in Western ChinaKey Laboratory of Resource Biology and Biotechnology in Western ChinaKey Laboratory of Resource Biology and Biotechnology in Western ChinaDepartment of OrthopedicsDepartment of OrthopedicsDepartment of HematologyDepartment of OrthopedicsBackground. Gastric cancer (GC) is the most common malignant tumor and ranks third in the world. LncRNA H19 (H19), one of the members of lncRNA, is overexpressed in various tumors. However, many undetermined molecular mechanisms by which H19 promotes GC progression still need to be further investigated. Methodology. A series of experiments was used to confirm the undetermined molecular mechanism including wound healing and transwell assays. Key Results. In this study, a significant upregulation of H19 expression was detected in GC cells and tissues. The poor overall survival was observed in GC patient with high H19 expression. Overexpression of H19 promoted the migration of GC cells, while knockdown of H19 significantly inhibited cell migration. Moreover, miR-148a-3p had a certain negative correlation with H19. Luciferase reporter assay confirmed that H19 could directly bind to miR-148a-3p. As expected, miR-148a mimics inhibited cell migration and invasion induced by H19 overexpression. The above findings proved that H19 functions as a miRNA sponge and verified that miR-148a-3p is the H19-associated miRNA in GC. We also confirmed that SOX-12 expression was upregulated in GC patient’s samples. SOX-12 expression was positively correlated with expression of H19 and was able to directly bind to miR-148a-3p. Importantly, in vitro wound healing assay showed that knockout of SOX-12 could reverse the promoting effect of H19 overexpression on cell migration. Conclusion. In conclusion, H19 has certain application value in the diagnosis and prognosis of GC. Specifically, H19 accelerates GCs to migration and metastasis by miR-138a-3p/SOX-12 axis.http://dx.doi.org/10.1155/2024/6217134 |
| spellingShingle | Xin Zhang Ge Wang Xiaoru Li Yanqing Liu Xue Wu Yazhe Zhou Jie Liu Haiying Wang Rui Jiao Ying Chen Qiang Wang LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis Analytical Cellular Pathology |
| title | LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis |
| title_full | LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis |
| title_fullStr | LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis |
| title_full_unstemmed | LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis |
| title_short | LncRNA H19 Promotes Gastric Cancer Metastasis via miR-148-3p/SOX-12 Axis |
| title_sort | lncrna h19 promotes gastric cancer metastasis via mir 148 3p sox 12 axis |
| url | http://dx.doi.org/10.1155/2024/6217134 |
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