Characterization of swine-origin H1N1 canine influenza viruses

Host switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 an...

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Main Authors: Guojun Wang, Luiz Gustavo dos Anjos Borges, Daniel Stadlbauer, Irene Ramos, Maria C. Bermúdez González, Jianqiao He, Yangbao Ding, Zuzhang Wei, Kang Ouyang, Weijian Huang, Viviana Simon, Ana Fernandez-Sesma, Florian Krammer, Martha I. Nelson, Ying Chen, Adolfo García-Sastre
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Emerging Microbes and Infections
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Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2019.1637284
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author Guojun Wang
Luiz Gustavo dos Anjos Borges
Daniel Stadlbauer
Irene Ramos
Maria C. Bermúdez González
Jianqiao He
Yangbao Ding
Zuzhang Wei
Kang Ouyang
Weijian Huang
Viviana Simon
Ana Fernandez-Sesma
Florian Krammer
Martha I. Nelson
Ying Chen
Adolfo García-Sastre
author_facet Guojun Wang
Luiz Gustavo dos Anjos Borges
Daniel Stadlbauer
Irene Ramos
Maria C. Bermúdez González
Jianqiao He
Yangbao Ding
Zuzhang Wei
Kang Ouyang
Weijian Huang
Viviana Simon
Ana Fernandez-Sesma
Florian Krammer
Martha I. Nelson
Ying Chen
Adolfo García-Sastre
author_sort Guojun Wang
collection DOAJ
description Host switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.
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spelling doaj-art-0058af6d80c04d42a68281f4a8e7f2af2025-08-20T02:09:02ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811017102610.1080/22221751.2019.1637284Characterization of swine-origin H1N1 canine influenza virusesGuojun Wang0Luiz Gustavo dos Anjos Borges1Daniel Stadlbauer2Irene Ramos3Maria C. Bermúdez González4Jianqiao He5Yangbao Ding6Zuzhang Wei7Kang Ouyang8Weijian Huang9Viviana Simon10Ana Fernandez-Sesma11Florian Krammer12Martha I. Nelson13Ying Chen14Adolfo García-Sastre15The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, People’s Republic of ChinaDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USADepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USADepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USADepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USACollege of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of ChinaDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USADepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USADepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USADivision of International Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, USACollege of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of ChinaDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USAHost switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.https://www.tandfonline.com/doi/10.1080/22221751.2019.1637284Host switchreassortantH1N1influenza A virusescanine
spellingShingle Guojun Wang
Luiz Gustavo dos Anjos Borges
Daniel Stadlbauer
Irene Ramos
Maria C. Bermúdez González
Jianqiao He
Yangbao Ding
Zuzhang Wei
Kang Ouyang
Weijian Huang
Viviana Simon
Ana Fernandez-Sesma
Florian Krammer
Martha I. Nelson
Ying Chen
Adolfo García-Sastre
Characterization of swine-origin H1N1 canine influenza viruses
Emerging Microbes and Infections
Host switch
reassortant
H1N1
influenza A viruses
canine
title Characterization of swine-origin H1N1 canine influenza viruses
title_full Characterization of swine-origin H1N1 canine influenza viruses
title_fullStr Characterization of swine-origin H1N1 canine influenza viruses
title_full_unstemmed Characterization of swine-origin H1N1 canine influenza viruses
title_short Characterization of swine-origin H1N1 canine influenza viruses
title_sort characterization of swine origin h1n1 canine influenza viruses
topic Host switch
reassortant
H1N1
influenza A viruses
canine
url https://www.tandfonline.com/doi/10.1080/22221751.2019.1637284
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