Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng
Glycopeptide from Ganoderma capense (Lloyd) Teng (GCGP) injection is widely used in kinds of immune disorders, but little is known about the molecular mechanisms of how GCGP could interfere with immune cell function. In the present study, we have found that GCGP had inflammatory modulation effects o...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/691285 |
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| author | Yan Zhou Song Chen Ran Ding Wenbing Yao Xiangdong Gao |
| author_facet | Yan Zhou Song Chen Ran Ding Wenbing Yao Xiangdong Gao |
| author_sort | Yan Zhou |
| collection | DOAJ |
| description | Glycopeptide from Ganoderma capense (Lloyd) Teng (GCGP) injection is widely used in kinds of immune disorders, but little is known about the molecular mechanisms of how GCGP could interfere with immune cell function. In the present study, we have found that GCGP had inflammatory modulation effects on macrophage cells to maintain NO production and iNOS expression at the normal level. Furthermore, western blot analysis showed that the underlying mechanism of immunomodulatory effect of GCGP involved NF-κB p65 translation, IκB phosphorylation, and degradation; NF-κB inhibitor assays also confirmed the results. In addition, competition study showed that GCGP could inhibit LPS from binding to macrophage cells. Our data indicates that GCGP, which may share the same receptor(s) expressed by macrophage cells with LPS, exerted immunomodulatory effect in a NF-κB-dependent signaling pathway in macrophages. |
| format | Article |
| id | doaj-art-00534c4b489d4b0199357b02527d3f2b |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-00534c4b489d4b0199357b02527d3f2b2025-08-20T02:03:14ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/691285691285Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) TengYan Zhou0Song Chen1Ran Ding2Wenbing Yao3Xiangdong Gao4State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, ChinaGlycopeptide from Ganoderma capense (Lloyd) Teng (GCGP) injection is widely used in kinds of immune disorders, but little is known about the molecular mechanisms of how GCGP could interfere with immune cell function. In the present study, we have found that GCGP had inflammatory modulation effects on macrophage cells to maintain NO production and iNOS expression at the normal level. Furthermore, western blot analysis showed that the underlying mechanism of immunomodulatory effect of GCGP involved NF-κB p65 translation, IκB phosphorylation, and degradation; NF-κB inhibitor assays also confirmed the results. In addition, competition study showed that GCGP could inhibit LPS from binding to macrophage cells. Our data indicates that GCGP, which may share the same receptor(s) expressed by macrophage cells with LPS, exerted immunomodulatory effect in a NF-κB-dependent signaling pathway in macrophages.http://dx.doi.org/10.1155/2014/691285 |
| spellingShingle | Yan Zhou Song Chen Ran Ding Wenbing Yao Xiangdong Gao Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng Mediators of Inflammation |
| title | Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng |
| title_full | Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng |
| title_fullStr | Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng |
| title_full_unstemmed | Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng |
| title_short | Inflammatory Modulation Effect of Glycopeptide from Ganoderma capense (Lloyd) Teng |
| title_sort | inflammatory modulation effect of glycopeptide from ganoderma capense lloyd teng |
| url | http://dx.doi.org/10.1155/2014/691285 |
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