ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR
Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast...
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2022-06-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202202&vol=2&aid=5430 |
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| author | DAN ZGARDAN IRINA MITINA VALENTIN MITIN EMILIA BEHTA SILVIA RUBTOV ALINA BOISTEAN RODICA STURZA MARIA MUNTEANU |
| author_facet | DAN ZGARDAN IRINA MITINA VALENTIN MITIN EMILIA BEHTA SILVIA RUBTOV ALINA BOISTEAN RODICA STURZA MARIA MUNTEANU |
| author_sort | DAN ZGARDAN |
| collection | DOAJ |
| description | Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit. |
| format | Article |
| id | doaj-art-002bdedfaab44ddba58dec552fc0a8fe |
| institution | Kabale University |
| issn | 1582-540X |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-002bdedfaab44ddba58dec552fc0a8fe2025-08-20T03:48:35ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X2022-06-01232179188ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCRDAN ZGARDAN0IRINA MITINA1 VALENTIN MITIN2EMILIA BEHTA3SILVIA RUBTOV4ALINA BOISTEAN5 RODICA STURZA6MARIA MUNTEANU7Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova The Institute of Genetics, Physiology and Plant Protection, 20, Padurilor Str., Chisinau MD-2002, Republic of Moldova The Institute of Genetics, Physiology and Plant Protection, 20, Padurilor Str., Chisinau MD-2002, Republic of MoldovaTechnical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of MoldovaTechnical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of MoldovaTechnical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of MoldovaTechnical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of MoldovaTechnical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of MoldovaAcetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit.https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202202&vol=2&aid=5430acetic acid bacteriadetection kitdnaprimersreal-time pcr |
| spellingShingle | DAN ZGARDAN IRINA MITINA VALENTIN MITIN EMILIA BEHTA SILVIA RUBTOV ALINA BOISTEAN RODICA STURZA MARIA MUNTEANU ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry acetic acid bacteria detection kit dna primers real-time pcr |
| title | ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR |
| title_full | ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR |
| title_fullStr | ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR |
| title_full_unstemmed | ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR |
| title_short | ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR |
| title_sort | acetic acid bacteria detection in wines by real time pcr |
| topic | acetic acid bacteria detection kit dna primers real-time pcr |
| url | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202202&vol=2&aid=5430 |
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