The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells
Cr(VI) exposure could produce kinds of intermediates and reactive oxygen species, both of which were related to DNA damage. Hyaluronan (HA) has impressive biological functions and was reported to protect corneal epithelial cells against oxidative damage induced by ultraviolet B, benzalkonium chlorid...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/3678586 |
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| author | Wei Wu Hua Jiang Xinnian Guo Yu Wang Shibo Ying Lingfang Feng Tao Li Hailing Xia Yixiao Zhang Riping Chen Tianhui Chen Jianlin Lou |
| author_facet | Wei Wu Hua Jiang Xinnian Guo Yu Wang Shibo Ying Lingfang Feng Tao Li Hailing Xia Yixiao Zhang Riping Chen Tianhui Chen Jianlin Lou |
| author_sort | Wei Wu |
| collection | DOAJ |
| description | Cr(VI) exposure could produce kinds of intermediates and reactive oxygen species, both of which were related to DNA damage. Hyaluronan (HA) has impressive biological functions and was reported to protect corneal epithelial cells against oxidative damage induced by ultraviolet B, benzalkonium chloride, and sodium lauryl sulfate. So the aim of our study was to investigate HA protection on human corneal epithelial (HCE) cells against Cr(VI)-induced toxic effects. The HCE cell lines were exposed to different concentrations of K2Cr2O7 (1.875, 3.75, 7.5, 15.0, and 30 μM) or a combination of K2Cr2O7 and 0.2% HA and incubated with different times (15 min, 30 min, and 60 min). Our data showed that Cr(VI) exposure could cause decreased cell viability, increased DNA damage, and ROS generation to the HCE cell lines. But incubation of HA increased HCE cell survival rates and decreased DNA damage and ROS generation induced by Cr(VI) in a dose- and time-dependent manner. We report for the first time that HA can protect HCE cells against the toxicity of Cr(VI), indicating that it will be a promising therapeutic agent to corneal injuries caused by Cr(VI). |
| format | Article |
| id | doaj-art-0012030ff0fa4005ad21c964ea3e5fcc |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-0012030ff0fa4005ad21c964ea3e5fcc2025-08-20T02:01:46ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/36785863678586The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial CellsWei Wu0Hua Jiang1Xinnian Guo2Yu Wang3Shibo Ying4Lingfang Feng5Tao Li6Hailing Xia7Yixiao Zhang8Riping Chen9Tianhui Chen10Jianlin Lou11The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaInstitute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, ChinaCr(VI) exposure could produce kinds of intermediates and reactive oxygen species, both of which were related to DNA damage. Hyaluronan (HA) has impressive biological functions and was reported to protect corneal epithelial cells against oxidative damage induced by ultraviolet B, benzalkonium chloride, and sodium lauryl sulfate. So the aim of our study was to investigate HA protection on human corneal epithelial (HCE) cells against Cr(VI)-induced toxic effects. The HCE cell lines were exposed to different concentrations of K2Cr2O7 (1.875, 3.75, 7.5, 15.0, and 30 μM) or a combination of K2Cr2O7 and 0.2% HA and incubated with different times (15 min, 30 min, and 60 min). Our data showed that Cr(VI) exposure could cause decreased cell viability, increased DNA damage, and ROS generation to the HCE cell lines. But incubation of HA increased HCE cell survival rates and decreased DNA damage and ROS generation induced by Cr(VI) in a dose- and time-dependent manner. We report for the first time that HA can protect HCE cells against the toxicity of Cr(VI), indicating that it will be a promising therapeutic agent to corneal injuries caused by Cr(VI).http://dx.doi.org/10.1155/2017/3678586 |
| spellingShingle | Wei Wu Hua Jiang Xinnian Guo Yu Wang Shibo Ying Lingfang Feng Tao Li Hailing Xia Yixiao Zhang Riping Chen Tianhui Chen Jianlin Lou The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells Journal of Ophthalmology |
| title | The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells |
| title_full | The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells |
| title_fullStr | The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells |
| title_full_unstemmed | The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells |
| title_short | The Protective Role of Hyaluronic Acid in Cr(VI)-Induced Oxidative Damage in Corneal Epithelial Cells |
| title_sort | protective role of hyaluronic acid in cr vi induced oxidative damage in corneal epithelial cells |
| url | http://dx.doi.org/10.1155/2017/3678586 |
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