Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis

In this work, we report the phenolic composition and bioactivity of the aerial parts of three species of Sarcocapnos (S. enneaphylla, S. pulcherrima, and S. saetabensis) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant sp...

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Main Authors: María del Pilar Fernández-Poyatos, Gökhan Zengin, Carlos Salazar-Mendías, Antonio Ruiz-Medina, Kouadio Ibrahime Sinan, Eulogio J. Llorent-Martínez
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2020/8885169
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author María del Pilar Fernández-Poyatos
Gökhan Zengin
Carlos Salazar-Mendías
Antonio Ruiz-Medina
Kouadio Ibrahime Sinan
Eulogio J. Llorent-Martínez
author_facet María del Pilar Fernández-Poyatos
Gökhan Zengin
Carlos Salazar-Mendías
Antonio Ruiz-Medina
Kouadio Ibrahime Sinan
Eulogio J. Llorent-Martínez
author_sort María del Pilar Fernández-Poyatos
collection DOAJ
description In this work, we report the phenolic composition and bioactivity of the aerial parts of three species of Sarcocapnos (S. enneaphylla, S. pulcherrima, and S. saetabensis) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant species. Samples were collected in different locations in the province of Jaén (southeast of Spain), and qualitative and quantitative analyses of phenolic compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detection. S. enneaphylla presented the highest concentration of phenolic compounds (58 mg/g DE). The most abundant compound in S. enneaphylla and S. saetabensis was rutin (35 mg/g DE and 11.7 mg/g DE, respectively), whereas isorhamnetin-O-rutinoside was dominant in S. pulcherrima (11.5 mg/g DE). Several assays were performed to evaluate the potential bioactivity of the three species of Sarcocapnos. These assays included antioxidant and radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, and enzyme inhibitory activity (acetylcholinesterase, amylase, butyrylcholinesterase, glucosidase, and tyrosinase). In general, all methanolic extracts presented the highest phenolic and flavonoid contents, as well as the highest radical scavenging, antioxidant, and enzyme inhibitory properties. This relationship between phenolics and bioactivity was confirmed by multivariate analysis.
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spelling doaj-art-000f808873f04211ae8d45b78c73fa5b2025-08-20T03:55:23ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88851698885169Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate AnalysisMaría del Pilar Fernández-Poyatos0Gökhan Zengin1Carlos Salazar-Mendías2Antonio Ruiz-Medina3Kouadio Ibrahime Sinan4Eulogio J. Llorent-Martínez5Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, Jaén E-23071, SpainDepartment of Biology, Science Faculty, Selcuk University, Campus, Konya, TurkeyDepartment of Animal Biology Plant Biology and Ecology, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, Jaén E-23071, SpainDepartment of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, Jaén E-23071, SpainDepartment of Biology, Science Faculty, Selcuk University, Campus, Konya, TurkeyDepartment of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, Jaén E-23071, SpainIn this work, we report the phenolic composition and bioactivity of the aerial parts of three species of Sarcocapnos (S. enneaphylla, S. pulcherrima, and S. saetabensis) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant species. Samples were collected in different locations in the province of Jaén (southeast of Spain), and qualitative and quantitative analyses of phenolic compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detection. S. enneaphylla presented the highest concentration of phenolic compounds (58 mg/g DE). The most abundant compound in S. enneaphylla and S. saetabensis was rutin (35 mg/g DE and 11.7 mg/g DE, respectively), whereas isorhamnetin-O-rutinoside was dominant in S. pulcherrima (11.5 mg/g DE). Several assays were performed to evaluate the potential bioactivity of the three species of Sarcocapnos. These assays included antioxidant and radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, and enzyme inhibitory activity (acetylcholinesterase, amylase, butyrylcholinesterase, glucosidase, and tyrosinase). In general, all methanolic extracts presented the highest phenolic and flavonoid contents, as well as the highest radical scavenging, antioxidant, and enzyme inhibitory properties. This relationship between phenolics and bioactivity was confirmed by multivariate analysis.http://dx.doi.org/10.1155/2020/8885169
spellingShingle María del Pilar Fernández-Poyatos
Gökhan Zengin
Carlos Salazar-Mendías
Antonio Ruiz-Medina
Kouadio Ibrahime Sinan
Eulogio J. Llorent-Martínez
Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis
Journal of Analytical Methods in Chemistry
title Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis
title_full Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis
title_fullStr Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis
title_full_unstemmed Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis
title_short Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis
title_sort study on three sarcocapnos species as potential sources of bioactive compounds relation between phenolic content and bioactivity by multivariate analysis
url http://dx.doi.org/10.1155/2020/8885169
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