Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay
Food allergies are a severe burden for affected individuals and healthcare systems. To tackle the need for simple food allergen detection, we developed a system for the detection of the soy protein glycinin via a centrifugal microfluidics-assisted lateral flow immunoassay (LFIA). Glycinin is a compl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-04-01
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| Series: | Proceedings |
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| Online Access: | https://www.mdpi.com/2504-3900/97/1/199 |
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| author | Bastian Breiner Daniel M. Kainz Stefan Wagner Maxime Gavage Serhat Sahakalkan Riccardo Marega Felix von Stetten Anna Klebes |
| author_facet | Bastian Breiner Daniel M. Kainz Stefan Wagner Maxime Gavage Serhat Sahakalkan Riccardo Marega Felix von Stetten Anna Klebes |
| author_sort | Bastian Breiner |
| collection | DOAJ |
| description | Food allergies are a severe burden for affected individuals and healthcare systems. To tackle the need for simple food allergen detection, we developed a system for the detection of the soy protein glycinin via a centrifugal microfluidics-assisted lateral flow immunoassay (LFIA). Glycinin is a complex allergen requiring extensive sample preparation. The presented workflow includes a manual denaturing extraction, followed by automated centrifugal microfluidic desalting, metering and detection via LFIA. The functionality of the microfluidic cassettes was tested on prototypes produced via microthermoforming before an injection molding tool was designed, which added a cylindrical lens to improve the readout. Overall, this system aims to aid in food allergen detection with high sensitivity and minimized manual steps. |
| format | Article |
| id | doaj-art-00098fa4d4474a7591ec60de5d3791a3 |
| institution | Kabale University |
| issn | 2504-3900 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proceedings |
| spelling | doaj-art-00098fa4d4474a7591ec60de5d3791a32024-12-27T14:49:08ZengMDPI AGProceedings2504-39002024-04-0197119910.3390/proceedings2024097199Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow AssayBastian Breiner0Daniel M. Kainz1Stefan Wagner2Maxime Gavage3Serhat Sahakalkan4Riccardo Marega5Felix von Stetten6Anna Klebes7Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, GermanyHahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, GermanyHahn-Schickard, Allmandring 9 b, 70569 Stuttgart, GermanyCER Groupe, Novalis Science Park, Rue de la Science 8, 6900 Aye, BelgiumHahn-Schickard, Allmandring 9 b, 70569 Stuttgart, GermanyCER Groupe, Novalis Science Park, Rue de la Science 8, 6900 Aye, BelgiumHahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, GermanyHahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, GermanyFood allergies are a severe burden for affected individuals and healthcare systems. To tackle the need for simple food allergen detection, we developed a system for the detection of the soy protein glycinin via a centrifugal microfluidics-assisted lateral flow immunoassay (LFIA). Glycinin is a complex allergen requiring extensive sample preparation. The presented workflow includes a manual denaturing extraction, followed by automated centrifugal microfluidic desalting, metering and detection via LFIA. The functionality of the microfluidic cassettes was tested on prototypes produced via microthermoforming before an injection molding tool was designed, which added a cylindrical lens to improve the readout. Overall, this system aims to aid in food allergen detection with high sensitivity and minimized manual steps.https://www.mdpi.com/2504-3900/97/1/199lateral flow assaycentrifugal microfluidicssample preparationallergen detection |
| spellingShingle | Bastian Breiner Daniel M. Kainz Stefan Wagner Maxime Gavage Serhat Sahakalkan Riccardo Marega Felix von Stetten Anna Klebes Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay Proceedings lateral flow assay centrifugal microfluidics sample preparation allergen detection |
| title | Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay |
| title_full | Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay |
| title_fullStr | Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay |
| title_full_unstemmed | Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay |
| title_short | Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay |
| title_sort | automated allergen sample preparation and detection via centrifugal microfluidic lateral flow assay |
| topic | lateral flow assay centrifugal microfluidics sample preparation allergen detection |
| url | https://www.mdpi.com/2504-3900/97/1/199 |
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