Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment

<b>Background:</b> Interleukin-10 (IL-10) and vascular endothelial growth factor (VEGF) are believed to possess a role in the pathophysiology of multiple myeloma (MM). We aimed to assess the significance of these parameters in the diagnosis, monitoring, and prognosis of the disease by ex...

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Main Authors: Fulya Memis, Meryem Yalvac Kandefer, Sonay Aydin, Klara Dalva, Selami Kocak Toprak
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Diagnostics
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Online Access:https://www.mdpi.com/2075-4418/15/13/1641
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author Fulya Memis
Meryem Yalvac Kandefer
Sonay Aydin
Klara Dalva
Selami Kocak Toprak
author_facet Fulya Memis
Meryem Yalvac Kandefer
Sonay Aydin
Klara Dalva
Selami Kocak Toprak
author_sort Fulya Memis
collection DOAJ
description <b>Background:</b> Interleukin-10 (IL-10) and vascular endothelial growth factor (VEGF) are believed to possess a role in the pathophysiology of multiple myeloma (MM). We aimed to assess the significance of these parameters in the diagnosis, monitoring, and prognosis of the disease by examining them in patients at diagnosis and post-treatment and comparing the findings with those of healthy individuals. <b>Methods:</b> We conducted blood sampling from 35 patients diagnosed with MM at the time of diagnosis and from 15 of these patients post-treatment. We additionally assessed similar serum markers in a control group of 15 healthy individuals. Furthermore, we documented laboratory results, organ involvement, comorbidities, and CD27-CD81 levels assessed using flow cytometry in the bone marrow, along with treatments and patient responses. We also examined the quantity of cells collected during mobilization in patients who had autologous stem cell transplantation. <b>Results:</b> We found a positive correlation (<i>p</i> = 0.028/<i>p</i> = 0.035) between IL-10 and VEGF with the international staging score. In patients with renal involvement, IL-10 levels were higher and VEGF levels were lower than those without renal involvement (<i>p</i> = 0.011/<i>p</i> = 0.012). We showed that VEGF levels decreased significantly with treatment (<i>p</i> = 0.001). We found no statistically significant correlation between treatment responses and IL-10 and VEGF. The number of CD34 cells collected by mobilization showed a negative correlation with CD27 and a positive correlation with VEGF (<i>p</i> = 0.007/<i>p</i> = 0.032). <b>Conclusions:</b> Serum IL-10 level is associated with ISS and renal involvement in MM patients. There is a positive correlation between serum VEGF levels and the number of stem cells collected during mobilization. As CD27 expression increases, the number of stem cells collected in mobilization decreases.
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spelling doaj-art-0000ca4506ed482ea49039ccd063c3d32025-08-20T03:16:43ZengMDPI AGDiagnostics2075-44182025-06-011513164110.3390/diagnostics15131641Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After TreatmentFulya Memis0Meryem Yalvac Kandefer1Sonay Aydin2Klara Dalva3Selami Kocak Toprak4Department of Internal Medicine, Faculty of Medicine, Erzincan Binali Yıldırım University, Erzincan 24100, TurkeyDepartment of Internal Medicine, Faculty of Medicine, Erzincan Binali Yıldırım University, Erzincan 24100, TurkeyDepartment of Radiology, Faculty of Medicine, Erzincan Binali Yıldırım University, Erzincan 24100, TurkeyDepartment of Hematology, Faculty of Medicine, Ankara University, Ankara 06620, TurkeyDepartment of Hematology, Faculty of Medicine, Ankara University, Ankara 06620, Turkey<b>Background:</b> Interleukin-10 (IL-10) and vascular endothelial growth factor (VEGF) are believed to possess a role in the pathophysiology of multiple myeloma (MM). We aimed to assess the significance of these parameters in the diagnosis, monitoring, and prognosis of the disease by examining them in patients at diagnosis and post-treatment and comparing the findings with those of healthy individuals. <b>Methods:</b> We conducted blood sampling from 35 patients diagnosed with MM at the time of diagnosis and from 15 of these patients post-treatment. We additionally assessed similar serum markers in a control group of 15 healthy individuals. Furthermore, we documented laboratory results, organ involvement, comorbidities, and CD27-CD81 levels assessed using flow cytometry in the bone marrow, along with treatments and patient responses. We also examined the quantity of cells collected during mobilization in patients who had autologous stem cell transplantation. <b>Results:</b> We found a positive correlation (<i>p</i> = 0.028/<i>p</i> = 0.035) between IL-10 and VEGF with the international staging score. In patients with renal involvement, IL-10 levels were higher and VEGF levels were lower than those without renal involvement (<i>p</i> = 0.011/<i>p</i> = 0.012). We showed that VEGF levels decreased significantly with treatment (<i>p</i> = 0.001). We found no statistically significant correlation between treatment responses and IL-10 and VEGF. The number of CD34 cells collected by mobilization showed a negative correlation with CD27 and a positive correlation with VEGF (<i>p</i> = 0.007/<i>p</i> = 0.032). <b>Conclusions:</b> Serum IL-10 level is associated with ISS and renal involvement in MM patients. There is a positive correlation between serum VEGF levels and the number of stem cells collected during mobilization. As CD27 expression increases, the number of stem cells collected in mobilization decreases.https://www.mdpi.com/2075-4418/15/13/1641monoclonal gammopathyangiogenesismobilizationplasma cellsinternational staging system
spellingShingle Fulya Memis
Meryem Yalvac Kandefer
Sonay Aydin
Klara Dalva
Selami Kocak Toprak
Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment
Diagnostics
monoclonal gammopathy
angiogenesis
mobilization
plasma cells
international staging system
title Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment
title_full Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment
title_fullStr Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment
title_full_unstemmed Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment
title_short Evaluation of Interleukin-10, Vascular Endothelial Growth Factor Levels, and Bone Marrow Parameters in Multiple Myeloma Patients at Diagnosis and After Treatment
title_sort evaluation of interleukin 10 vascular endothelial growth factor levels and bone marrow parameters in multiple myeloma patients at diagnosis and after treatment
topic monoclonal gammopathy
angiogenesis
mobilization
plasma cells
international staging system
url https://www.mdpi.com/2075-4418/15/13/1641
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