Keeping Active with Texting after Stroke (KATS): a single-arm feasibility and acceptability study of a behavioural intervention to promote community-based physical activity after s... by Gozde Ozakinci, Stephan U Dombrowski, Andrew Murphy, Albert Farre, Jenna Breckenridge, Jacqui H Morris, Linda A Irvine, Keith Jenkinson

“…Based on these findings, KATS will be further optimised before evaluating effectiveness in a randomised controlled trial.Trial registration number and protocol availability ISRCTN 13704805 https://www.hra.nhs.uk/planning-and-improving-research/application-summaries/research-summaries/keeping-active-with-texting-after-stroke-kats/Protocol available https://www.isrctn.com/ISRCTN13704805?q=13704805&filters=&sort=&offset=1&totalResults=1&page=1&pageSize=10…”
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Effectiveness of flipped classrooms in Chinese students of clinical medicine major undergoing clinical practice: a meta-analysis by Xinrui Sun, Xin Yuan, Linyu Zhang, Yi Zhang, Xue Xiao, Hanmin Liu, Fang Ma

“…Weighted mean differences (WMD) and 95% confidence interval (CI) were used to compare the differences in theoretical and skill scores between the flipped classrooms and traditional lecture groups. The Cochran Q test and the I2 statistic were used for heterogeneity analysis. …”
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Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus by Jiang Ye, Xiaowei Wu, Xiang Li, Yuting Zhang, Yuqing Li, Hui Zhang, Jie Chen, Yuyan Xiang, Yefan Xia, Hu Zhao, Zengdong Tan, Liang Guo, Xuan Yao

“…Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.MYB52 transcription factor as a candidate gene underlying ZS11_qSCC.A09. Knocking out BnaMYB52 results in a 7.7%–8.2% reduction in seed coat content (SCC) but a 12.3%–13.4% increase in SOC, whereas overexpression of BnaA09.MYB52 leads to the opposite effects. …”
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Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4 by Rongyu Shi, Ning Zhang, Han Li, Hu Zhong, Chengcheng Zhong, Wei Du, Xi Yang

“…Levels of COL17A1 and Actinin Alpha 4 (ACTN4) were measured by using qRT-PCR and western blotting. Functional analyses were conducted using MTT, 5-ethynyl-2ʹ-deoxyuridine, transwell, and sphere formation assays, respectively. …”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. …”
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FBR2 modulates ferroptosis via the SIRT3/p53 pathway to ameliorate pulmonary fibrosis by Yu Cheng, Yang Jiao, Wan Wei, Mengjia Kou, Yaodong Cai, Yang Li, Hao Li, Tonghua Liu

“…Assessments encompassed histopathology, ELISA for cytokine detection, IHC and Western blot for protein expression analysis, and qRT-PCR for gene expression quantification. Transmission electron microscopy (TEM) was used to observe mitochondrial morphology. …”
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Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction by Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Cao

“…Key findings were validated by RT-qPCR and immunofluorescence. The Connectivity Map (CMap) database was queried to predict potential POCD treatments. …”
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Heterogeneous occurrence of evergreen broad-leaved forests in East Asia: Evidence from plant fossils by Jiagang Zhao, Shufeng Li, Jian Huang, Wenna Ding, Mengxiao Wu, Tao Su, Alexander Farnsworth, Paul J. Valdes, Linlin Chen, Yaowu Xing, Zhekun Zhou

“…Paleoclimate simulation results suggest that the precipitation of wettest quarter (PWetQ, mm) exceeding 600 mm is crucial for the occurrence of EBLFs. …”
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Economic Evaluation of Robotic-assisted Radical Prostatectomy: A Systematic Review and Meta-analysis by Tanan Bejrananda, Win Khaing, Sajesh K. Veettil, Therdpong Thongseiratch, Nathorn Chaiyakunapruk

“…Heterogeneity was assessed using the Q test and I2 statistic. Key findings and limitations: Thirteen studies with 17 comparisons, ten from high-income (HICs) and three from middle-income (MICs) countries, were included. …”
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Unveiling prognostic value of JAK/STAT signaling pathway related genes in colorectal cancer: a study of Mendelian randomization analysis by Nan Zhang, Wenli Yue, Bihang Jiao, Duo Cheng, Jingjing Wang, Fang Liang, Yingnan Wang, Xiyue Liang, Kunkun Li, Junwei Liu, Yadong Li

“…The expressions of prognostic genes were verified by RT-qPCR. Then, a risk model was built and validated by the GSE39582. …”
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Olfactory mucosa-mesenchymal stem cells with overexpressed Nrf2 modulate angiogenesis and exert anti-inflammation effect in an in vitro traumatic brain injury model by Zigui Chen, Chunyuan Zhang, Yuhua Fang, He Zhang, Jiawei Luo, Changfeng Miao, Jiale Li, Jun Peng, Yingqi Qiu, Ying Xia, Qisheng Luo

“…Immunofluorescence assay was applied to determine the effects of OM-MSCsNrf2 on microglial polarization, and the underlying molecular mechanisms were analyzed based on the quantification tests of RT-qPCR and immunoblotting. Results Co-culture of OM-MSCsNrf2 and PC12 cells increased the levels of anti-inflammatory cytokines and pro-angiogenesis factors, enhanced the cell survival and angiogenesis. …”
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UBE2T/CDC42/CD276 signaling axis mediates brain metastasis of triple-negative breast cancer via lysosomal autophagy by Lin Li, Rong Fu, Yan Chen, Jin Yao, Xiaobing Jiang, Tao Liang, Jun jun Li, Qian zhi Chen, Liang Liang Shi, Ming Xing Xie, Xin Wei Qiao, Qi Hong Cheng, Min Jie Wang

“…Additionally, multiple experimental approaches were implemented, encompassing western blotting, Co-IP, GST pull-down, flow cytometry, mass spectrometry, immunofluorescence, immunohistochemistry, and qRT-PCR to reveal the molecular mechanism of UBE2T-mediated immune escape and BrM.Results Our results indicate that expressed at elevated levels in breast cancer, UBE2T is negatively linked to patient prognosis, especially in BrM of TNBC. …”
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Machine Learning Diagnostic Model for Hepatocellular Carcinoma Based on Liquid–Liquid Phase Separation and Ferroptosis-Related Genes by Wenchao Chen, Ting Zhu, Xiaofan Pu, Linlin Zhao, Senhao Zhou, Xin Zhong, Suihan Wang, Tianyu Lin

“…Based on central hub genes, a diagnostic model was developed through LASSO regression and validated using KM survival analysis and real-time quantitative polymerase chain reaction (RT-qPCR). Then the effects of NRAS on the development of HCC and ferroptosis were also detected. …”
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Evaluation of exo-long noncoding RNA MALAT1 in OSCC in comparison to dysplastic and normal: A cross-sectional study by Ramya Sekar, Selvaraj Jayaraman, VishnuPriya Veeraraghavan, Kalaiselvi Krishnamoorthy, Shubhra Chauhan Aramanai, Sathan Raj Natarajan

“…RNA concentration was estimated, real-time polymerase chain reaction (qPCR) was used for quantitative analysis. Gene expression levels of MALAT1, PI3K, AKT1, and PTEN were analysed and compared across groups using one way ANOVA and Post-hoc Tukey analysis was performed for pairwise comparisons to assess correlations between MALAT1 expression and PI3K/AKT pathway components. …”
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Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease by Xiaozhu Ma, Shuai Mei, Yi He, Qidamugai Wuyun, Li Zhou, Ziyang Cai, Qiushi Luo, Yi Wen, Jiangtao Yan

“…Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) was performed to assess miR-146b-5p expression in PBMCs. …”
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Integrating Bulk and Single-Cell Transcriptomic Data to Identify Ferroptosis-Associated Inflammatory Gene in Alzheimer’s Disease by Zhou H, Peng Y, Huo X, Li B, Liu H, Wang J, Zhang G

“…Finally, candidate genes were validated in ferroptosis models using reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence methods.Results: Bulk RNA-seq analysis identified SLC11A1, an inflammatory gene associated with AD. …”
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CircPRMT5, a Potential Salivary Biomarker, Facilitates the Progression of Head and Neck Squamous Cell Carcinoma via the IGF2BP3-SERPINE1 Pathway by Jiang S, Ou L, Wang Y, Su K, Chen Z, He L, Xu X, Cheng B, Xia J, Fan Z

“…Finally, salivary exosomes were isolated, and the expression levels of circPRMT5 were assessed using qRT-PCR.Results: The upregulation of circPRMT5 in HNSCC tissues was identified to be correlated with cervical lymph node metastasis and advanced clinical T stage. …”
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Environmental surveillance of SARS-CoV-2 for outbreak detection in hospital: A single centre prospective study by Hania Siddiqui, Alexandra M.A. Hicks, Aaron Hinz, Prachi Ray, Jennie Johnstone, Derek R. MacFadden, Jason A. Moggridge, Michael Fralick

“…Swabs were assayed for SARS-CoV-2 by quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR). Results from the floor swabs, including percentage positivity for SARS-CoV-2 and number of viral RNA copies, were sent to the hospital's infection control team twice-weekly. …”
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Placental malaria infection is associated with downregulation of STAT-6 and ANG-1 in decidual macrophages by Fred Owino, Fred Owino, Fred Owino, Caroline Kijogi, Caroline Kijogi, Caroline Kijogi, Omu Anzala, Omu Anzala, Edwin Walong, Obiero Jael, Steven G. Nyanjom, Agola Lelo Eric, Bernard N. Kanoi, Bernard N. Kanoi, Jesse Gitaka, Jesse Gitaka

“…For gene expression profiling, macrophages were isolated from the placental immune cells and the expression level of transcription factors STAT-1, IRF-5, STAT-6, c-Maf and angiogenic factors ANG-1, ANG-2 and VEGF determined by qPCR.Results and DiscussionWe found no difference in the total macrophage populations and M1 and M2 macrophage profiles between uninfected and infected placentas, however, M2 macrophages were significantly higher compared to their M1 counterparts regardless of infection status. …”
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Peroral endoscopic myotomy with fundoplication (POEM-F) for achalasia: Systematic review and meta-analysis by Harishankar Gopakumar, Eugene Annor, Ishaan Vohra, Iman Andalib, Amy Tyberg, Avik Sarkar, Haroon Shahid, Mine Carames, Juan Carlos Carames, Giovanna Porfilio Gularte, Abed Al-Lehibi, Resheed Alkhiari, Amol Bapaye, Carlos Robles-Medranda, Michel Kahaleh

“…Heterogeneity was assessed using I2 and Q statistics. Results: We included seven studies comprising 127 patients. …”
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