Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…To determine the mechanism of efferocytosis, we analysed the number of macrophages by immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR). Results The injection of enucleated BMSCs (Cargocytes) into the testes of EDS-treated rats restored the levels of serum testosterone, increased the number of Leydig cells (LCs), and improved spermatogenesis. …”
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Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings by CHEN Yan, HAN Liyuan, DU Zilong, ZHU Dongxing, ZHU Weimin, ZHANG Yingying

“…The expression of heat responsive genes, antioxidant, and ABA-related genes were analyzed by qRT-PCR. [Results] 20 mmol/L myo-inositol treatment could effectively alleviate the damage of high temperature stress on tomato seedlings, reduce the heat damage index of tomato leaves, increase the maximum photochemical efficiency (Fv/Fm), and significantly increased the content of photosynthetic pigments. …”
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A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice by Guohao Han, Dan Li, Haisen Zhang, Chao Li, Luda Yang, Tiantian Ma, Xuerong Wang, Bairong Ma, Xiaodie Wu, Yang Tao, Ziang Wang, Aihua Wang, Hsu-Wen Chao, Yaping Jin, Huatao Chen

“…Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. …”
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Role and mechanism of dexmedetomidine in regulating bone metabolism in tail-suspended osteoporotic rats by GUO Yunliang, WANG Can, ZHANG Xinyu

“…Secondly, after primary osteoblasts were isolated from the tibiae of tail-suspended rats and then treated with 1 nmol/L Dex, the proportion of alkaline phosphatase (ALP)-positive osteoblasts and the activity of the enzyme were detected by ALP staining and activity test. qRT-PCR was applied to measure the expression of osteogenic activity-related factors, including osteocalcin (Ocn), Runt related transcription factor 2 (Runx2), Osterix protein (Osx), and type 1 collagen (Col1). …”
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Safety of Drinking Water from Primary Water Sources and Implications for the General Public in Uganda by Keneth Iceland, Kasozi, Sarah, Namubiru, Roland, Kamugisha, Ejike, Daniel Eze, Fred, Ssempijja, Alfred, Omachonu Okpanachi, Hellen, Wambui Kinyi, Jovile Kasande, Atusiimirwe, Dickson Stuart, Tayebwa, Joy, Suubo, Edgar Mario, Fernandez, Nathan, Nshakira, Andrew, Tamale

“…A spatial map was drawn using qGIS®, and analysis of quantitative data was done using MS Excel 2013 at 95% significance. …”
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Integrated microbiome, transcriptome and metabolome insight into the alleviating mechanisms of sheep milk in a DSS-induced colitis mouse model by Wenna Yao, Ruilin Du, Shuo Yan, Teligun Bao, Huimin Zhang, Fang Yang, Yue Xue, Yulong Zhao, Siqin Bao, Xihe Li, Yongli Song

“…ELISA and qPCR analysis showed that relative mRNA expression levels of TNF-α, IL-1β, IL-6, and IL-17 were decreased in SM treatment compared to DSS-induced colitis mice. …”
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Silicon photonic modulators with a 2 × 1 Fabry–Perot cavity by Cao Hengzhen, Xie Jin, Sun Weichao, Zhu Mingyu, Xiang Yuluan, Zhang Gong, Guo Jingshu, Shi Yaocheng, Dai Daoxin

“…In particular, the straight modulation section in the FP cavity is broadened to be far beyond the single-mode regime, alleviating the inherent sensitivity to the variations of waveguide dimensions and thus reducing stochastic resonance-wavelength variations. The Q factor of the FP cavity is manipulated by optimally manipulating the reflection of the AMWGs, and the modulation bandwidth is enhanced to be over 40 GHz by utilizing the optical peaking enhancement effect, which happens when operating at the wavelength slightly detuning to its resonance wavelength. …”
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Informing grassland ecosystem modeling with in-situ and remote sensing observations by Johny Arteaga, Melannie D Hartman, William J Parton, Maosi Chen, Wei Gao

“…The model’s phenology was informed by the MODIS MCD12Q2 product, which showed good agreement with in-situ observations of growing season commencement and duration across different grassland ecosystems, and with observed historical trends. …”
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Experimental study of the piezoelectricity of Middle Pleistocene loess in the Weibei region, China by Yanlin JING, Jie LIU, Qingying LIU, Xiaoxiao DUAN, Na XIE, Dengke YANG

“…MethodsThis study investigated the \begin{document}${\mathrm{Q}}_2^{{\mathrm{eol}}} $\end{document} loess from the Weibei Loess Tableland in Shaanxi Province as an example to explore the piezoelectricity of loess. …”
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Next-generation calcium oxide nanoparticles: A breakthrough in energy storage and humidity sensing by Asif Khan, Syed Tasleem Hussain, Abdul Naeem, Ayesha Sadiqa, Awais Ahmad, Muhammad Aamir Ali Shehzada, Munirah D. Albaqami

“…Humidity sensor measurements were performed by determining changes in resistance of the sensing material applying LCR (Q) meter due to changes in humidity levels in a close chamber. …”
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Expectativas acerca das atividades síncronas na formação a distância do Programa Mais Médicos para o Brasil by Inara Pereira da Cunha, Sílvia Helena Mendonça de Moraes, Adriana Carvalho dos Santos, Débora Dupas Gonçalves do Nascimento

“…As respostas dos 722 estudantes e dos 72 tutores foram analisadas pelo software IRaMuTeQ, com tratamento lexical, classificação hierárquica descendente (CHD), análise fatorial por correspondência (AFC) e análise de similitude. …”
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Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation by Cao R, Jing J, Ma Y, Qi W, Huang X, Zhang C, Lu Z, He J, Wang G, Ma Y, Zhang H

“…Enzyme-linked immunosorbent assay (ELISA), Reverse transcription - quantitative polymerase chain reaction (RT-qPCR), Western blotting (WB), immunohistochemistry, and immunofluorescence were used to detect NLRP3 inflammasome activation in vivo. …”
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Over-Expression of MicroRNA-210 and MicroRNA-185-5p in Normotensive Late-Fetal Growth Restriction: Preliminary Cohort Study by Esra Altan Erbilen, Gulizar Fusun Varol, Necdet Sut, Nebiye Pelin Turker, Niyazi Cenk Sayin

“…We determined these two miRNAs using Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) and statistically analyzed them with clinical data to determine robustness. …”
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Characterization of polyethylene and polyurethane microplastics and their adsorption behavior on Cu2+ and Fe3+ in environmental matrices by Ilaria Zanoni, Lucia Briccolani, Lara Faccani, Magda Blosi, Simona Ortelli, Matteo Crosera, Giovanna Marussi, Stefania Albonetti, Anna Luisa Costa

“…We observed selective adsorption for Cu2⁺ ions, which was both medium-dependent (more ions adsorbed in Elendt M7) and plastic-dependent, with PU showing a stronger affinity for Cu2⁺ in MilliQ and Egg water. On the contrary, both plastics showed similar adsorption capacity for Fe3⁺ ions across all media.…”
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Plasma Metabolite Profiles of Healthy Volunteers after Administration of a Thai Herbal Formula for Dizziness by Ranida Boonrak, Kajee Pilakasiri, Suksalin Boonranasubkajorn, Natchagorn Lumlerdkij, Pravit Akarasereenont

“…Phytochemical and metabolite profiling were performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and LC/MS Q-ToF, respectively, on plasma samples from 90 healthy participants aged 20 to 60 years. …”
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Role of Aging in Ulcerative Colitis Pathogenesis: A Focus on ETS1 as a Promising Biomarker by Ni M, Peng W, Wang X, Li J

“…Finally, the expression levels of key genes were determined in a dextran sulfate sodium (DSS)-induced UC mouse model by qRT-PCR.Results: UC samples were classified into two subtypes (1 and 2), which displayed significant differences in the immune landscape and JAK/STAT signaling pathways. …”
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GelMA@LNP/AST Promotes eNOS-Dependent Angiogenesis Through Autophagy Activation for the Treatment of Hind Limb Ischemia by Lai L, Wu H, Peng L, Zhang Z, Wu X, Zheng S, Su Z, Chu H

“…The changes in angiogenesis markers, autophagy markers, and eNOS levels were detected using qPCR and Western blotting. 3-MA was used to assess the role of autophagy in the activation of eNOS mediated by AST and its subsequent angiogenic effects.Results: GelMA@LNP/AST significantly promoted blood flow recovery in mice with hind limb ischemia. …”
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FoxA1 knockdown promotes BMSC osteogenesis in part by activating the ERK1/2 signaling pathway and preventing ovariectomy-induced bone loss by Lijun Li, Renjin Lin, Yang Xu, Lingdi Li, Zhijun Pan, Jian Huang

“…The expression of genes involved in osteogenic differentiation was examined via qRT‒PCR. Lentiviral transfection was utilized to suppress the expression of the FOXA1 gene in hBMSCs. …”
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Toward High-order Solar Corona Simulations: A High-order Hyperbolized Poisson Approach for Magnetic Field Initialization by Rayan Dhib, Firas Ben Ameur, Vatsalya Sharma, Andrea Lani, Stefaan Poedts

“…Our contributions include developing a Q 2 (i.e., quadratic) geometrical representation using prismatic elements for the computational domain, which enables high-order mesh generation. …”
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Vincristine Regulates C/EBP-β/TGF-β1 to Promote A1 Astrocyte Polarization and Induce Neuropathic Pain by Chen Y, Tang G, Lu J, Tang S, Xiong X, Chen C, Pei L, Shi J

“…Pain threshold of rats was measured by thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT), Elisa was used to detect the expression level of inflammatory factors, qRT PCR and Western blotting were used to detect astrocyte polarization markers, C/EBP-β, TGF-β 1, p-smad2 and p-smad3.Results: Following Vincristine administration, the TWL and MWT of rats exhibited a decrease. …”
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