Correlation between malondialdehyde level and FOXO3 and CASP3 mRNA expression changed in early-onset preeclampsia placenta by Ni Made Wiasty Sukanty, Febriana Catur Iswanti, Syarifah Dewi, Muhammad Faruqi, Alyssa Shafa Andiana, Ani Retno Prijanti

“…A total of 31 EOPE placentas and 31 normal term placentas were used to measure the concentration of malondialdehyde (MDA) and the relative mRNA expression of FOXO3 and CASP3 using the spectrophotometric and RT-qPCR methods. Results: There was no difference in MDA concentration (p = 0.580) and FOXO3 (p = 0.467) and CASP3 (p = 0.243) mRNA expression in the normal and EOPE groups. …”
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Omicron Positivity in Air of Hospital Settings Gathered COVID-19 Patients, Vaccinated/Unvaccinated Populations by Zeynep Ece Kuloğlu, Zeynep Bengi Eren, Bedirhan Haykar, Cansel Vatansever, Tayfun Barlas, Mert Ahmet Kuskucu, Gülen Güney-Esken, Füsun Can

“…In the cross-sectional part, we collected aerosol samples from clinical and public areas in the hospital. We performed qPCR and viral culture. In the pediatrics, outpatient clinic waiting room, where the children were without masks, and unvaccinated, 66% of the samples were positive for viral RNA. …”
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Putative and pretreatment drug resistance mutations in reverse transcriptase gene among untreated chronic hepatitis B patients at Arifin Achmad Regional District Hospital, Riau, In... by Arfianti Arfianti, Fauzia Andrini Djojosugito, Maisaroh Maisaroh, Hendra Asputra, Dita Kartika Sari, Tubagus Odih Rhomdani Wahid

“…In addition, F221Y and N238H/Q mutations were frequently observed in genotype B, while V224 I was only found in patients infected with genotype C (p=0.000). …”
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Effect of glyphosate on renal function: A study integrating epidemiological and experimental evidence by Lin Hu, Mingcong Chen, Xiaoran Xue, Mingyi Zhao, Qingnan He

“…The underlying mechanism of the association between glyphosate and renal function was explored by the real-time quantitative polymerase chain reaction (RT-qPCR) and non-targeted metabolomics analysis [embryos (0.75 hpf–96 hpf) were exposed to 90 μg/mL glyphosate]. 1170 participants were enrolled in the NHANES study. …”
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CircSPG21 ameliorates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells and mitigates intervertebral disc degeneration through the miR-217/SIRT... by Yongbo Zhang, Sheng Yang, Xuan You, Zhengguang Li, Liuyang chen, Rui Dai, Hua Sun, Liang Zhang

“…Interactions among circSPG21, miR-217, and the NAD-dependent protein deacetylase sirtuin-1 (SIRT1) were validated through dual-luciferase assays, RNA fluorescence in situ hybridization and RNA immune precipitation. β-Gal staining, EdU staining, Western blotting, JC-1 assays, cell cycle analysis, and quantitative reverse transcription PCR (RT‒qPCR) were used to examine the functions of these molecules in TBHP-induced senescent NPMSCs. …”
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Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 by Adel, Eltoukhy, Yang, Jia, Imane, Lamraoui, M. A., Abo‑Kadoum, Omar, Mohammad Atta, Ruth, Nahurira, Junhuan, Wang, Yanchun, Yan

“…The pathways of microbial metabolism in diverse environments were significantly enriched among DEGs based on KEGG enrichment analysis. qRT‑PCR confirm the involvement of BPA degradation relevant genes in accordance with RNA Seq data. …”
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Cefazolin Alone Versus Cefazolin With Tobramycin or Gentamicin as Intraoperative Antibiotic Prophylaxis for Total Joint Arthroplasty by Meera M. Dhodapkar, MD, MHS, Scott J. Halperin, MD, MHS, Zachary Radford, MD, Lee E. Rubin, MD, FAAOS, FAOA, Jonathan N. Grauer, MD, Mengnai Li, FAAOS, MD, PhD

“…Methods: Adult primary THA and TKA patients were identified from 2010-2021 Q1 PearlDiver M151 database. Inclusion criteria were activity in the database ≥ 90 days postoperative and no infectious, neoplastic, or traumatic diagnoses within 90 days preoperative. …”
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Blue applicability grade index assessment and response surface modelling to synchronous determination of metformin hydrochloride, vildagliptin and dapagliflozin propanediol monohyd... by Pintu Prajapati, Krutika Patil, Dhrumi Naik, Veera Shakar Pulusu, Anzarul Haque, Mohd Abul Kalam, Shailesh Shah

“…The chromatographic method was validated as per ICH Q2 (R2) guideline. The developed method was applied for synchronous assay of multiple combinations of metformin, vildagliptin and dapagliflozin using single chromatographic condition to save time, cost and resources for analysis. …”
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Association between the rifampicin resistance mutations and rifabutin susceptibility in Mycobacterium tuberculosis: A meta-analysis by Wenli Wang, Hongjuan Zhou, Long Cai, Tingting Yang

“…Among these, nine had high confidence (OR>10) in predicting RFB susceptibility: RpoB D435V, H445L, D435Y, D435F, S441L, L430P, H445G, S441Q, L430R. Among the strains carrying these mutations, 59.04% (702/1189) were susceptible to RFB. …”
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Promoting ER stress in a plasmacytoid dendritic cell line drives fibroblast activation by Beatriz H. Ferreira, Inês S. Silva, Andreia Mendes, Fátima Leite-Pinheiro, Adrienne W. Paton, James C. Paton, Iola F. Duarte, Philippe Pierre, Catarina R. Almeida

“…Extracellular matrix (ECM) production was assessed using immunoblotting, qPCR and confocal microscopy. The importance of cell-to-cell contact was investigated using conditioned media (CM) and transwell assays. …”
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Triatoma venosa and Panstrongylus geniculatus challenge the certification of interruption of vectorial Trypanosoma cruzi transmission by Rhodnius prolixus in eastern Colombia. by Omar Cantillo-Barraza, Lídia Gual-González, Natalia Velásquez-Ortiz, Manuel Alfonso Medina Camargo, Paola González, Lissa Cruz-Saavedra, Adriana Castillo, Sara Zuluaga, Giovanny Herrera, Hanson Cowan, Andrés Velez-Mira, Luz Helena Patiño, Juan David Ramírez, Omar Triana, Melissa S Nolan

“…No evidence of T. cruzi DNA was found using qPCR in human blood samples, but we found high parasitemia levels in the infected dogs. …”
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Laparoscopic Sacrocolpopexy or Sacrohysteropexy with or Without Burch Colposuspension: The Results of 36 Patients in Our Clinic by İlknur İnegöl Gümüş, Önder Sürgit, Nilüfer Akgün, İkbal Kaygusuz, Deniz Hızlı

“…Demographic and clinical data, intraoperative findings and postoperative course were recorded. POP-Q examination was used for pelvic prolapsed patients. …”
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Breeding for Heat Tolerant Aromatic Rice Varieties and Identification of Novel QTL Regions Associated with Heat Tolerance During Reproductive Phase by QTL-Seq by Surangkana Chimthai, Sulaiman Cheabu, Wanchana Aesomnuk, Siriphat Ruengphayak, Siwaret Arikit, Apichart Vanavichit, Chanate Malumpong

“…Regarding the QTL-seq results, the qSF2.1 region ranged from 311 051 to 3 929 422 bp on chromosome 2, was identified based on the highest contrasting SNP index between the heat-susceptible and tolerant bulks. …”
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Causal association between non-thyroidal autoimmune diseases and Graves' ophthalmopathy: A mendelian randomization study by Lan Ma, Xue Jiang, Zhijia Hou, Dongmei Li

“…Bidirectional MR analysis was conducted using inverse variance weighted (IVW) method, weighted median (WM) method and MR-Egger test. Cochran's Q statistic was used to assess the heterogeneity between SNP estimates. …”
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Chemical characterization of Siraitia grosvenorii granules and their efficacy and mechanism of action on PM2.5-induced acute lung injury by Qi Shao, Yan Zhao, YuYu Shi, Fafeng Cheng, ZeHan Zhang, Ying Liu, ChangXiang Li, ZiLin Ren, Hua Bai, Haojie Cheng, Rolando Maddela, Junqiang Tian, Xueqian Wang

“…The chemical composition of SGG, such as wogonin (MOL000173), luteolin (MOL000006), nobiletin (MOL005828), naringenin (MOL004328), acacetin (MOL001689), were identified via ultra-high-performance liquid chromatography-Q Exactive (UHPLC-QE) Orbitrap/MS. The specific targets and pathways through which the compounds exert their effects on acute lung injury were then predicted via network pharmacology. …”
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Evaluation of cellular and physiological alterations of cells from Mytilus galloprovincialis exposed to benzisothiazolinone by Federica Impellitteri, Kristian Riolo, Giorgia Zicarelli, Miriam Porretti, Cristiana Roberta Multisanti, Giuseppe Piccione, Alessia Giannetto, Caterina Faggio

“…Moreover, the cytoprotective mechanisms related to oxidative stress and detoxification were evaluated through the expression analysis by qPCR of key genes involved in these pathways. The most significant results obtained from these tests were the reduction of viability of haemocytes at T2 of exposure to B2, a decrease of vitality in DG cells exposed to B1 during the T1, the significant alteration in the phagocytosis activity, the upregulation of Cu/ZnSOD gene, the inhibition of the CYPY1 gene expression and upregulation of the Heat shock protein 70 (Hsp70) gene following the BIT exposure. …”
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CircHIPK2 recruits SRSF1 to increase TXNIP mRNA stability and promotes autophagy-dependent ferroptosis and apoptosis in myocardial ischemia-reperfusion injury by Zhu Zhang, Jinqi Hao, Qinghong Qiao, Junting Song, Yanqin Yu

“…Expression of circHIPK2, SRSF1 and TXNIP was assessed using RT-qPCR. Protein levels of autophagy markers (LC3II/LC3I, Beclin1, p62) and ferroptosis markers (GPX4, FTH1, ACSL4) were detected by Western blot. …”
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Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages by Ivana Beatrice Mânica da Cruz, Cibele Ferreira Teixeira, Neida Luiza Pellenz, Moisés Henrique Mastella, Verônica Farina Azzolin, Euler Esteves Ribeiro, Fernanda Barbisan

“…The effects on cell proliferation rate and the concentration and production of transcripts of pro-inflammatory cytokines interleukin 1β (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), as well as the anti-inflammatory cytokine IL-10, were assessed using spectrophotometric, immunoassay, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) techniques. Results: S. adstringens extract at all concentrations tested here reduced the cellular proliferation rate of activated macrophages. …”
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The Spatio-temporal Evolution of NDVI in the Three Gorges Reservoir Area Driven by the Synergistic Effect of two Factors over the Past 21 Years by QI Haimei, ZHENG Peilong, WANG Yunqi, WANG Jiani, LI Cheng, ZHANG Xiaoming

“…[Results] (1) The change of NDVI in the Three Gorges Reservoir Area showed an overall ascending trend from 2000 to 2020, with a mean annual rate of increase at 2.89×10-3/a, and the value of NDVI increased from the southwestern to the northeastern parts of the area. (2) Elevation, population density, and surface temperature are pivotal factors that significantly account for the variability observed in NDVI, with an explanatory power for each exceeding the threshold of 0.4. (3) Elevation and nocturnal illumination are the key interactive drivers of NDVI in the Three Gorges Reservoir Area, with a pronounced q value of 0.641. Their interplay showed a nonlinear or synergistic enhancement, and the integrated explanatory power of the two factors on NDVI changes is always greater than that of single factors. …”
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Enteric-Coated Aspirin Induces Small Intestinal Injury via the Nrf2/Gpx4 Pathway: A Promising Model for Chronic Enteropathy by Zhang M, Xia S, Feng L, Han X, Zhang Y, Huang Y, Liu Y, Zhao K, Guan J, Tian D, Liao J, Yu Y

“…The mRNA and protein levels of inflammatory factors, tight junctions, and antioxidant defense-related genes were measured by qRT-PCR and Western Blot. The MPO activity, SOD activity and MDA content in serum were measured. …”
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