A CRISPR-Cas9 system for knock-out and knock-in of high molecular weight DNA enables module-swapping of the pikromycin synthase in its native host

A CRISPR-Cas9 system for knock-out and knock-in of high molecular weight DNA enables module-swapping of the pikromycin synthase in its native host

Abstract Background Engineers seeking to generate natural product analogs through altering modular polyketide synthases (PKSs) face significant challenges when genomically editing large stretches of DNA. Results We describe a CRISPR-Cas9 system that was employed to reprogram the PKS in Streptomyces...

Full description

Saved in:
Bibliographic Details
Main Authors: Zhe-Chong Wang, Hayden Stegall, Takeshi Miyazawa, Adrian T. Keatinge-Clay
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Microbial Cell Factories
Subjects:
Pikromycin
Type I PKSs
CRISPR/Cas9
Riboswitch
Updated module boundary
Module-swapping
Online Access:https://doi.org/10.1186/s12934-025-02741-w
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doi.org/10.1186/s12934-025-02741-w

Similar Items