An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance
Tumor necrosis factor alpha (TNF-α)/hypoxia-treated 3T3-L1 adipocytes have been used to model inflamed and insulin-resistant adipose tissue: this study examines gaps in the model. We tested whether modulating TNF-α/hypoxia treatment time could reduce cell death while still inducing inflammation and...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2024.2414919 |
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| author | Ifeoluwa A. Odeniyi Bulbul Ahmed Benjamin Anbiah Grace Hester Peter T. Abraham Elizabeth A. Lipke Michael W. Greene |
| author_facet | Ifeoluwa A. Odeniyi Bulbul Ahmed Benjamin Anbiah Grace Hester Peter T. Abraham Elizabeth A. Lipke Michael W. Greene |
| author_sort | Ifeoluwa A. Odeniyi |
| collection | DOAJ |
| description | Tumor necrosis factor alpha (TNF-α)/hypoxia-treated 3T3-L1 adipocytes have been used to model inflamed and insulin-resistant adipose tissue: this study examines gaps in the model. We tested whether modulating TNF-α/hypoxia treatment time could reduce cell death while still inducing inflammation and insulin resistance. Adipocytes were treated with TNF-α (12 h or 24 h) and incubated in a hypoxic chamber for 24 h. To examine maintenance of the phenotype over time, glucose and FBS were added at 24 h post initiation of treatment, and the cells were maintained for an additional 48 h. Untreated adipocytes were used as a control. Viability, insulin resistance, and inflammation were assessed using Live/Dead staining, RT-qPCR, ELISA, and glucose uptake assays. Treatment for 12 h with TNF-α in the presence of hypoxia resulted in an increase in the percentage of live cells compared to 24 h treated cells. Importantly, insulin resistance and inflammation were still induced in the 12 h treated adipocytes: the expression of the insulin sensitive and inflammatory genes was decreased and increased, respectively. In 72 h treated adipocytes, no significant differences were found in the viability, glucose uptake or insulin-sensitive and inflammatory gene expression. This study provides a modified approach to in vitro odeling adipocyte inflammation and insulin resistance. |
| format | Article |
| id | doaj-art-fc2de475e9e44ab38af824874cbf36c1 |
| institution | Kabale University |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-fc2de475e9e44ab38af824874cbf36c12024-12-09T07:23:12ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2024-12-0113110.1080/21623945.2024.2414919An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistanceIfeoluwa A. Odeniyi0Bulbul Ahmed1Benjamin Anbiah2Grace Hester3Peter T. Abraham4Elizabeth A. Lipke5Michael W. Greene6Department of Nutritional Sciences, Auburn University, Auburn, AL, USADepartment of Nutritional Sciences, Auburn University, Auburn, AL, USADepartment of Chemical Engineering, Auburn University, Auburn, AL, USADepartment of Chemical Engineering, Auburn University, Auburn, AL, USADepartment of Chemical Engineering, Auburn University, Auburn, AL, USADepartment of Chemical Engineering, Auburn University, Auburn, AL, USADepartment of Nutritional Sciences, Auburn University, Auburn, AL, USATumor necrosis factor alpha (TNF-α)/hypoxia-treated 3T3-L1 adipocytes have been used to model inflamed and insulin-resistant adipose tissue: this study examines gaps in the model. We tested whether modulating TNF-α/hypoxia treatment time could reduce cell death while still inducing inflammation and insulin resistance. Adipocytes were treated with TNF-α (12 h or 24 h) and incubated in a hypoxic chamber for 24 h. To examine maintenance of the phenotype over time, glucose and FBS were added at 24 h post initiation of treatment, and the cells were maintained for an additional 48 h. Untreated adipocytes were used as a control. Viability, insulin resistance, and inflammation were assessed using Live/Dead staining, RT-qPCR, ELISA, and glucose uptake assays. Treatment for 12 h with TNF-α in the presence of hypoxia resulted in an increase in the percentage of live cells compared to 24 h treated cells. Importantly, insulin resistance and inflammation were still induced in the 12 h treated adipocytes: the expression of the insulin sensitive and inflammatory genes was decreased and increased, respectively. In 72 h treated adipocytes, no significant differences were found in the viability, glucose uptake or insulin-sensitive and inflammatory gene expression. This study provides a modified approach to in vitro odeling adipocyte inflammation and insulin resistance.https://www.tandfonline.com/doi/10.1080/21623945.2024.2414919Adipocyteinflammationinsulin resistancein vitro model3T3-L1 cells |
| spellingShingle | Ifeoluwa A. Odeniyi Bulbul Ahmed Benjamin Anbiah Grace Hester Peter T. Abraham Elizabeth A. Lipke Michael W. Greene An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance Adipocyte Adipocyte inflammation insulin resistance in vitro model 3T3-L1 cells |
| title | An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance |
| title_full | An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance |
| title_fullStr | An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance |
| title_full_unstemmed | An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance |
| title_short | An improved in vitro 3T3-L1 adipocyte model of inflammation and insulin resistance |
| title_sort | improved in vitro 3t3 l1 adipocyte model of inflammation and insulin resistance |
| topic | Adipocyte inflammation insulin resistance in vitro model 3T3-L1 cells |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2024.2414919 |
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