Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities
Lignin is a complex chemical heterogeneous polymer that forms a physical barrier to lignocellulose's biological and chemical hydrolysis, making lignocellulosic biomass challenging to degrade. Ligninolytic microorganisms play an essential role in lignin degradation by producing extracellular en...
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Bogor Agricultural University
2024-11-01
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| Series: | Jurnal Ilmu Pertanian Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/JIPI/article/view/51883 |
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| author | Taruna Dwi Satwika Dwiana Muflihah Yulianti Galang Anahatta Hidayat Afifah Mariana |
| author_facet | Taruna Dwi Satwika Dwiana Muflihah Yulianti Galang Anahatta Hidayat Afifah Mariana |
| author_sort | Taruna Dwi Satwika |
| collection | DOAJ |
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Lignin is a complex chemical heterogeneous polymer that forms a physical barrier to lignocellulose's biological and chemical hydrolysis, making lignocellulosic biomass challenging to degrade. Ligninolytic microorganisms play an essential role in lignin degradation by producing extracellular enzymes. Lignin peroxidase and manganese peroxidase are enzymes that play a role in lignin degradation. Forty-one bacterial isolates have been isolated from soil, kitchen waste, leaf litter, and cow dung. However, the ligninolytic activity of these isolates has yet to be discovered. This research aimed to determine the ligninolytic ability of bacteria isolated from soil, leaf litter, kitchen waste, and cow dung based on lignin peroxidase and manganese peroxidase activity. The study was conducted stages: isolate recultured, qualitative and quantitative testing of lignin peroxidase activity based on degradation of methylene blue dye, and qualitative and quantitative testing of manganese peroxidase activity based on degradation of phenol red dye. A total of four bacterial isolates from soil (Tn9, Tn14, Tn16, and Tn17) and two bacterial isolates from cow dung (KS2 and KS5) showed qualitative and quantitative lignin peroxidase activity. Manganese peroxidase activity was also shown by four isolates from soil (Tn2, Tn6, Tn14, and Tn16), one isolate from kitchen waste (SD1), and one isolate from cow dung (KS5) both qualitatively and quantitatively. The nine bacterial isolates that showed lignin peroxidase and manganese peroxidase activity have potential as lignin-degrading biological agents.
Keywords: bacteria, ligninolytic, peroxidase
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| format | Article |
| id | doaj-art-fc0334e537c145de90c0143abb1ffb23 |
| institution | Kabale University |
| issn | 0853-4217 2443-3462 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Ilmu Pertanian Indonesia |
| spelling | doaj-art-fc0334e537c145de90c0143abb1ffb232024-12-30T06:52:37ZengBogor Agricultural UniversityJurnal Ilmu Pertanian Indonesia0853-42172443-34622024-11-0130110.18343/jipi.30.1.163Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase ActivitiesTaruna Dwi Satwika0Dwiana Muflihah Yulianti1Galang Anahatta Hidayat2Afifah Mariana3Faculty of Biology, General Soedirman University, Purwokerto 53123, IndonesiaFaculty of Biology, General Soedirman University, Purwokerto 53123, IndonesiaFaculty of Biology, General Soedirman University, Purwokerto 53123, IndonesiaFaculty of Biology, General Soedirman University, Purwokerto 53123, Indonesia Lignin is a complex chemical heterogeneous polymer that forms a physical barrier to lignocellulose's biological and chemical hydrolysis, making lignocellulosic biomass challenging to degrade. Ligninolytic microorganisms play an essential role in lignin degradation by producing extracellular enzymes. Lignin peroxidase and manganese peroxidase are enzymes that play a role in lignin degradation. Forty-one bacterial isolates have been isolated from soil, kitchen waste, leaf litter, and cow dung. However, the ligninolytic activity of these isolates has yet to be discovered. This research aimed to determine the ligninolytic ability of bacteria isolated from soil, leaf litter, kitchen waste, and cow dung based on lignin peroxidase and manganese peroxidase activity. The study was conducted stages: isolate recultured, qualitative and quantitative testing of lignin peroxidase activity based on degradation of methylene blue dye, and qualitative and quantitative testing of manganese peroxidase activity based on degradation of phenol red dye. A total of four bacterial isolates from soil (Tn9, Tn14, Tn16, and Tn17) and two bacterial isolates from cow dung (KS2 and KS5) showed qualitative and quantitative lignin peroxidase activity. Manganese peroxidase activity was also shown by four isolates from soil (Tn2, Tn6, Tn14, and Tn16), one isolate from kitchen waste (SD1), and one isolate from cow dung (KS5) both qualitatively and quantitatively. The nine bacterial isolates that showed lignin peroxidase and manganese peroxidase activity have potential as lignin-degrading biological agents. Keywords: bacteria, ligninolytic, peroxidase https://journal.ipb.ac.id/index.php/JIPI/article/view/51883 |
| spellingShingle | Taruna Dwi Satwika Dwiana Muflihah Yulianti Galang Anahatta Hidayat Afifah Mariana Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities Jurnal Ilmu Pertanian Indonesia |
| title | Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities |
| title_full | Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities |
| title_fullStr | Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities |
| title_full_unstemmed | Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities |
| title_short | Selection of Lignin Degrading Bacteria from Soil, Kitchen Waste, Leaf Litter, and Cow Dung Based on Lignin Peroxidase and Manganese Peroxidase Activities |
| title_sort | selection of lignin degrading bacteria from soil kitchen waste leaf litter and cow dung based on lignin peroxidase and manganese peroxidase activities |
| url | https://journal.ipb.ac.id/index.php/JIPI/article/view/51883 |
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