Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis

Objective: This study aimed to optimize the extraction process of Porphyra haitanensis polyphenols to develop natural hypoglycemic foods. Methods: The Box-Behnken response surface method was used to optimize the extraction process of P. haitanensis polyphenols. The α-glucosidase inhibition activity...

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Main Authors: CHEN Xiao-chen, DU Xi-ping, ZHOU Li-juan, WU Ling, LI Zhi-peng, YANG Yuan-fan
Format: Article
Language:English
Published: The Editorial Office of Food and Machinery 2023-04-01
Series:Shipin yu jixie
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Online Access:http://www.ifoodmm.com/spyjxen/article/abstract/20230223
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author CHEN Xiao-chen
DU Xi-ping
ZHOU Li-juan
WU Ling
LI Zhi-peng
YANG Yuan-fan
author_facet CHEN Xiao-chen
DU Xi-ping
ZHOU Li-juan
WU Ling
LI Zhi-peng
YANG Yuan-fan
author_sort CHEN Xiao-chen
collection DOAJ
description Objective: This study aimed to optimize the extraction process of Porphyra haitanensis polyphenols to develop natural hypoglycemic foods. Methods: The Box-Behnken response surface method was used to optimize the extraction process of P. haitanensis polyphenols. The α-glucosidase inhibition activity was applied to evaluate the hypoglycemic activity of P. haitanensis polyphenols at different harvest periods, and the antioxidant activities of them were evaluated based on DPPH, ABTS<sup>+</sup> radical scavenging rate and ORAC value. Results: The optimal extraction process conditions were liquid-solid ratio (V<sub>ethanol</sub>∶m<sub>P. haitanensis</sub>) 51∶1, ethanol concentration 56% and ultrasonication time 51 min. Under the control of these conditions, the extracted content of polyphenols was 5.027 mg/g. The inhibition of α-glucosidase activity by P. haitanensis polyphenols was in a concentration-dependent manner, and the inhibition by the fourth-harvest-period polyphenols was significantly higher than other harvest periods polyphenols. P. haitanensis polyphenols at different harvest periods showed antioxidant activity, and the antioxidant activity of the fourth-harvest-period polyphenols was strongest. Conclusion: The high polyphenol contents, strong inhibition of α-glucosidase activity and strong antioxidant capacity of the fourth-harvest-period makes it an excellent food source for developing natural hypoglycemic foods and antioxidants.
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spelling doaj-art-f2d7ee47ab5f4df7bf73c4ed09dcde5e2025-08-20T02:22:37ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-04-0139214014610.13652/j.spjx.1003.5788.2022.80537Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensisCHEN Xiao-chen0DU Xi-ping1ZHOU Li-juan2WU Ling3LI Zhi-peng4YANG Yuan-fan5 College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021 , China College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021 , China College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021 , China College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021 , China College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021 , China College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021 , China Objective: This study aimed to optimize the extraction process of Porphyra haitanensis polyphenols to develop natural hypoglycemic foods. Methods: The Box-Behnken response surface method was used to optimize the extraction process of P. haitanensis polyphenols. The α-glucosidase inhibition activity was applied to evaluate the hypoglycemic activity of P. haitanensis polyphenols at different harvest periods, and the antioxidant activities of them were evaluated based on DPPH, ABTS<sup>+</sup> radical scavenging rate and ORAC value. Results: The optimal extraction process conditions were liquid-solid ratio (V<sub>ethanol</sub>∶m<sub>P. haitanensis</sub>) 51∶1, ethanol concentration 56% and ultrasonication time 51 min. Under the control of these conditions, the extracted content of polyphenols was 5.027 mg/g. The inhibition of α-glucosidase activity by P. haitanensis polyphenols was in a concentration-dependent manner, and the inhibition by the fourth-harvest-period polyphenols was significantly higher than other harvest periods polyphenols. P. haitanensis polyphenols at different harvest periods showed antioxidant activity, and the antioxidant activity of the fourth-harvest-period polyphenols was strongest. Conclusion: The high polyphenol contents, strong inhibition of α-glucosidase activity and strong antioxidant capacity of the fourth-harvest-period makes it an excellent food source for developing natural hypoglycemic foods and antioxidants.http://www.ifoodmm.com/spyjxen/article/abstract/20230223 porphyra haitanensis polyphenols hypoglycemia α-glucosidase antioxidant activity different harvest periods extraction process optimization
spellingShingle CHEN Xiao-chen
DU Xi-ping
ZHOU Li-juan
WU Ling
LI Zhi-peng
YANG Yuan-fan
Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis
Shipin yu jixie
porphyra haitanensis
polyphenols
hypoglycemia
α-glucosidase
antioxidant activity
different harvest periods
extraction process optimization
title Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis
title_full Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis
title_fullStr Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis
title_full_unstemmed Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis
title_short Optimization of polyphenols process and their hypoglycemic and antioxidant activities of Porphyra haitanensis
title_sort optimization of polyphenols process and their hypoglycemic and antioxidant activities of porphyra haitanensis
topic porphyra haitanensis
polyphenols
hypoglycemia
α-glucosidase
antioxidant activity
different harvest periods
extraction process optimization
url http://www.ifoodmm.com/spyjxen/article/abstract/20230223
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