Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress

Background/Aim. Exertional heat stress is common problem in military services. The aim was to examine changes in serum concentrations of some enzymes in soldiers during exertional heat stress test (EHST) as well as the effects of 10-days passive or active acclimatization in climatic chamber. Methods...

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Main Authors: Radaković Sonja, Marić Jelena, Šurbatović Maja, Vasiljević Nađa, Milivojević Mladen
Format: Article
Language:English
Published: Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade 2009-01-01
Series:Vojnosanitetski Pregled
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Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2009/0042-84500905359R.pdf
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author Radaković Sonja
Marić Jelena
Šurbatović Maja
Vasiljević Nađa
Milivojević Mladen
author_facet Radaković Sonja
Marić Jelena
Šurbatović Maja
Vasiljević Nađa
Milivojević Mladen
author_sort Radaković Sonja
collection DOAJ
description Background/Aim. Exertional heat stress is common problem in military services. The aim was to examine changes in serum concentrations of some enzymes in soldiers during exertional heat stress test (EHST) as well as the effects of 10-days passive or active acclimatization in climatic chamber. Methods. Forty male soldiers with high aerobic capacity, performed EHST either in cool (20 ºC, 16 ºC Wet bulb globe temperature - WBGT), or hot (40 ºC, 25 ºC WBGT) environment, unacclimatized, or after 10 days of passive or active acclimation. Physiological strain was measured by tympanic temperatures (Tty) and heart rates (HR). Concentrations of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine-kinase (CK) were measured in blood samples collected before and immediately after EHST. Results. Exertional heat stress test in hot conditions induced physiological heat stress (increase in Tty and HR), with significant increase in concentrations of all enzymes in unacclimatized group: ALT (42.5 ± 4.2 before vs 48.1 ± 3.75 U/L after EHST, p < 0.01), AST (24.9 ± 5.1 vs 33.4 ± 4.48 U/L, p < 0.01), LDH (160.6 ± 20.2 vs 195.7 ± 22.6 U/L, p < 0.001) and CK (215.5 ± 91.2 vs 279.1 ± 117.5 U/L, p < 0.05). In acclimatized soldiers there were no significant changes in concentrations of ALT and AST, while concentration of CK was significantly higher. Concentrations of LDH were significantly higher in all investigated groups, regardless of temperature conditions. Conclusion. In trained soldiers, 10-days passive or active acclimatization in climatic chamber can prevent increase in serum concentrations of ALT and AST, induced by exertional heat stress. Increase of serum concentrations of CK and LDH was induced by physical strain itself, with no additional effect of heat stress.
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spelling doaj-art-eee79e33713e48d6a3f53d16c6c1bffa2025-08-20T03:06:30ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502009-01-0166535936410.2298/VSP0905359RInfluence of acclimatization on serum enzyme changes in soldiers during exertional heat stressRadaković SonjaMarić JelenaŠurbatović MajaVasiljević NađaMilivojević MladenBackground/Aim. Exertional heat stress is common problem in military services. The aim was to examine changes in serum concentrations of some enzymes in soldiers during exertional heat stress test (EHST) as well as the effects of 10-days passive or active acclimatization in climatic chamber. Methods. Forty male soldiers with high aerobic capacity, performed EHST either in cool (20 ºC, 16 ºC Wet bulb globe temperature - WBGT), or hot (40 ºC, 25 ºC WBGT) environment, unacclimatized, or after 10 days of passive or active acclimation. Physiological strain was measured by tympanic temperatures (Tty) and heart rates (HR). Concentrations of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine-kinase (CK) were measured in blood samples collected before and immediately after EHST. Results. Exertional heat stress test in hot conditions induced physiological heat stress (increase in Tty and HR), with significant increase in concentrations of all enzymes in unacclimatized group: ALT (42.5 ± 4.2 before vs 48.1 ± 3.75 U/L after EHST, p < 0.01), AST (24.9 ± 5.1 vs 33.4 ± 4.48 U/L, p < 0.01), LDH (160.6 ± 20.2 vs 195.7 ± 22.6 U/L, p < 0.001) and CK (215.5 ± 91.2 vs 279.1 ± 117.5 U/L, p < 0.05). In acclimatized soldiers there were no significant changes in concentrations of ALT and AST, while concentration of CK was significantly higher. Concentrations of LDH were significantly higher in all investigated groups, regardless of temperature conditions. Conclusion. In trained soldiers, 10-days passive or active acclimatization in climatic chamber can prevent increase in serum concentrations of ALT and AST, induced by exertional heat stress. Increase of serum concentrations of CK and LDH was induced by physical strain itself, with no additional effect of heat stress.http://www.doiserbia.nb.rs/img/doi/0042-8450/2009/0042-84500905359R.pdfacclimatizationmilitary personnelenzymesserumheat stress disordersexertion
spellingShingle Radaković Sonja
Marić Jelena
Šurbatović Maja
Vasiljević Nađa
Milivojević Mladen
Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
Vojnosanitetski Pregled
acclimatization
military personnel
enzymes
serum
heat stress disorders
exertion
title Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
title_full Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
title_fullStr Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
title_full_unstemmed Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
title_short Influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
title_sort influence of acclimatization on serum enzyme changes in soldiers during exertional heat stress
topic acclimatization
military personnel
enzymes
serum
heat stress disorders
exertion
url http://www.doiserbia.nb.rs/img/doi/0042-8450/2009/0042-84500905359R.pdf
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AT maricjelena influenceofacclimatizationonserumenzymechangesinsoldiersduringexertionalheatstress
AT surbatovicmaja influenceofacclimatizationonserumenzymechangesinsoldiersduringexertionalheatstress
AT vasiljevicnađa influenceofacclimatizationonserumenzymechangesinsoldiersduringexertionalheatstress
AT milivojevicmladen influenceofacclimatizationonserumenzymechangesinsoldiersduringexertionalheatstress