In vitro evaluation of agro-industrial by-products in diets for cattle

The objective of this in vitro batch culture study was to evaluate various agro-industrial by-products as feeds for cattle. In Experiment 1, a basal diet composed of grass silage and barley grain was replaced by additional barley grain, palm kernel cake (PKC), beet molasses (M), wheat bran (WB), or...

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Main Authors: Sophie Julie Krizsan, Marcia Franco, Marketta Rinne, Mohammad Ramin, Farhang Fatehi
Format: Article
Language:English
Published: Scientific Agricultural Society of Finland 2025-06-01
Series:Agricultural and Food Science
Subjects:
Online Access:https://journal.fi/afs/article/view/149470
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author Sophie Julie Krizsan
Marcia Franco
Marketta Rinne
Mohammad Ramin
Farhang Fatehi
author_facet Sophie Julie Krizsan
Marcia Franco
Marketta Rinne
Mohammad Ramin
Farhang Fatehi
author_sort Sophie Julie Krizsan
collection DOAJ
description The objective of this in vitro batch culture study was to evaluate various agro-industrial by-products as feeds for cattle. In Experiment 1, a basal diet composed of grass silage and barley grain was replaced by additional barley grain, palm kernel cake (PKC), beet molasses (M), wheat bran (WB), or sugar beet pulp (SBP) at two levels of dietary supplementation. In Experiment 2, soybean meal (SBM) was compared with heat-treated rapeseed meal, dried distillers´ grains (DDG), rapeseed cake (RSC), and rapeseed meal (RSM) at two levels of dietary protein concentration in diets based on grass silage supplemented with barley grain or SBP. Propionate and branched chain volatile fatty acids decreased, and CH4 production increased when energy by-products of higher fibre content replaced barley grain. Diets incorporating PKC and WB were less fermentable, while M and SBP did not alter digestibility when replacing barley. Generally, incremental levels of protein in Experiment 2 linearly increased digestibility, utilisable crude protein (uCP), isobutyrate and valerate in vitro. Utilisable crude protein increased for all by-products that replaced SBM, except for RSM in diets including SBP. There was a positive linear effect of by-product level on uCP and valerate, and a negative linear effect for acetate. Diet digestibility was equivalent for DDG and SBM in diets composed with barley as well as SBP. Overall, by-products provided more uCP than diets supplemented with SBM. However, the intestinal digestibility of uCP of the different feeds can vary. In vivo production experiments are needed to fully assess the potential of in vitro evaluated by-products as widely applicable alternative feeds in diets for cattle.
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spelling doaj-art-ec7ead67e1b94e6fa7dc7d8428f4e2212025-08-20T03:31:23ZengScientific Agricultural Society of FinlandAgricultural and Food Science1459-60671795-18952025-06-0110.23986/afsci.149470In vitro evaluation of agro-industrial by-products in diets for cattleSophie Julie Krizsan0Marcia Franco1Marketta Rinne2Mohammad Ramin3Farhang Fatehi4Inland Norway University of Applied SciencesNatural Resources Institute Finland (Luke)Natural Resources Institute Finland (Luke)Swedish University of Agricultural Sciences (SLU)University of TehranThe objective of this in vitro batch culture study was to evaluate various agro-industrial by-products as feeds for cattle. In Experiment 1, a basal diet composed of grass silage and barley grain was replaced by additional barley grain, palm kernel cake (PKC), beet molasses (M), wheat bran (WB), or sugar beet pulp (SBP) at two levels of dietary supplementation. In Experiment 2, soybean meal (SBM) was compared with heat-treated rapeseed meal, dried distillers´ grains (DDG), rapeseed cake (RSC), and rapeseed meal (RSM) at two levels of dietary protein concentration in diets based on grass silage supplemented with barley grain or SBP. Propionate and branched chain volatile fatty acids decreased, and CH4 production increased when energy by-products of higher fibre content replaced barley grain. Diets incorporating PKC and WB were less fermentable, while M and SBP did not alter digestibility when replacing barley. Generally, incremental levels of protein in Experiment 2 linearly increased digestibility, utilisable crude protein (uCP), isobutyrate and valerate in vitro. Utilisable crude protein increased for all by-products that replaced SBM, except for RSM in diets including SBP. There was a positive linear effect of by-product level on uCP and valerate, and a negative linear effect for acetate. Diet digestibility was equivalent for DDG and SBM in diets composed with barley as well as SBP. Overall, by-products provided more uCP than diets supplemented with SBM. However, the intestinal digestibility of uCP of the different feeds can vary. In vivo production experiments are needed to fully assess the potential of in vitro evaluated by-products as widely applicable alternative feeds in diets for cattle. https://journal.fi/afs/article/view/149470barley, by-products, methane, protein, soybean meal, utilisable crude protein
spellingShingle Sophie Julie Krizsan
Marcia Franco
Marketta Rinne
Mohammad Ramin
Farhang Fatehi
In vitro evaluation of agro-industrial by-products in diets for cattle
Agricultural and Food Science
barley, by-products, methane, protein, soybean meal, utilisable crude protein
title In vitro evaluation of agro-industrial by-products in diets for cattle
title_full In vitro evaluation of agro-industrial by-products in diets for cattle
title_fullStr In vitro evaluation of agro-industrial by-products in diets for cattle
title_full_unstemmed In vitro evaluation of agro-industrial by-products in diets for cattle
title_short In vitro evaluation of agro-industrial by-products in diets for cattle
title_sort in vitro evaluation of agro industrial by products in diets for cattle
topic barley, by-products, methane, protein, soybean meal, utilisable crude protein
url https://journal.fi/afs/article/view/149470
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AT markettarinne invitroevaluationofagroindustrialbyproductsindietsforcattle
AT mohammadramin invitroevaluationofagroindustrialbyproductsindietsforcattle
AT farhangfatehi invitroevaluationofagroindustrialbyproductsindietsforcattle