NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29
IntroductionGrain filling is a crucial stage of the rice endosperm development. During this process, the endosperm accumulates abundant storage products such as starch and proteins, which determine both the yield and quality of the grain.MethodsHere, we analyzed the expression of NAC25 transcription...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1563065/full |
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| author | Rong Li Rong Li Ming-Wei Wu Ming-Wei Wu Jinxin Liu Xintong Xu Yiqun Bao Chun-Ming Liu Chun-Ming Liu Chun-Ming Liu |
| author_facet | Rong Li Rong Li Ming-Wei Wu Ming-Wei Wu Jinxin Liu Xintong Xu Yiqun Bao Chun-Ming Liu Chun-Ming Liu Chun-Ming Liu |
| author_sort | Rong Li |
| collection | DOAJ |
| description | IntroductionGrain filling is a crucial stage of the rice endosperm development. During this process, the endosperm accumulates abundant storage products such as starch and proteins, which determine both the yield and quality of the grain.MethodsHere, we analyzed the expression of NAC25 transcription factor via qRT-PCR and histochemical GUS assays, and obtained its mutants by CRISPR/Cas9-based gene editing in ZH11.Results and discussionThe results showed that NAC25 was expressed specifically in developing rice endosperm, and knockout of NAC25 led to delayed degeneration of cytoplasmic membrane integrity, reduced starch accumulation and chalky starchy endosperm. We showed that NAC25 interacted with MADS29, a MADS family transcription factor whose mutant also showed defective grain filling. These results provide novel insight into the transcriptional regulation of rice grain filling. |
| format | Article |
| id | doaj-art-e9f6dae17ec9449db2ff48b9a32f5f4e |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-e9f6dae17ec9449db2ff48b9a32f5f4e2025-08-20T03:01:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15630651563065NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29Rong Li0Rong Li1Ming-Wei Wu2Ming-Wei Wu3Jinxin Liu4Xintong Xu5Yiqun Bao6Chun-Ming Liu7Chun-Ming Liu8Chun-Ming Liu9College of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaSchool of Advanced Agricultural Sciences, Peking University, Beijing, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaIntroductionGrain filling is a crucial stage of the rice endosperm development. During this process, the endosperm accumulates abundant storage products such as starch and proteins, which determine both the yield and quality of the grain.MethodsHere, we analyzed the expression of NAC25 transcription factor via qRT-PCR and histochemical GUS assays, and obtained its mutants by CRISPR/Cas9-based gene editing in ZH11.Results and discussionThe results showed that NAC25 was expressed specifically in developing rice endosperm, and knockout of NAC25 led to delayed degeneration of cytoplasmic membrane integrity, reduced starch accumulation and chalky starchy endosperm. We showed that NAC25 interacted with MADS29, a MADS family transcription factor whose mutant also showed defective grain filling. These results provide novel insight into the transcriptional regulation of rice grain filling.https://www.frontiersin.org/articles/10.3389/fpls.2025.1563065/fulltranscription factorNAC25MADS29starch synthesiscytoplasmic membrane degenerationrice grain filling |
| spellingShingle | Rong Li Rong Li Ming-Wei Wu Ming-Wei Wu Jinxin Liu Xintong Xu Yiqun Bao Chun-Ming Liu Chun-Ming Liu Chun-Ming Liu NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29 Frontiers in Plant Science transcription factor NAC25 MADS29 starch synthesis cytoplasmic membrane degeneration rice grain filling |
| title | NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29 |
| title_full | NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29 |
| title_fullStr | NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29 |
| title_full_unstemmed | NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29 |
| title_short | NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29 |
| title_sort | nac25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with mads29 |
| topic | transcription factor NAC25 MADS29 starch synthesis cytoplasmic membrane degeneration rice grain filling |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1563065/full |
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