Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy
Background/Aim. It is known that some members of the genus Convolvulus (C) L. are commonly used in Turkish folk medicine. These species are powerful in curing toothache and joint pains. Due to the limited information on the biological activities of C. aucheri, a species almost exclusively occurring...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2022-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502000133O.pdf |
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| author | Ozay Cennet Mammadov Ramazan |
| author_facet | Ozay Cennet Mammadov Ramazan |
| author_sort | Ozay Cennet |
| collection | DOAJ |
| description | Background/Aim. It is known that some members of the genus Convolvulus (C) L. are commonly used in Turkish folk medicine. These species are powerful in curing toothache and joint pains. Due to the limited information on the biological activities of C. aucheri, a species almost exclusively occurring in Turkey, we aimed to investigate the antioxidant and cytotoxic effects of three extracts obtained from the plant, as well as to characterize their phenolic profile. Methods. The anti-oxidant activity of the extracts was determined by using ABTS, NO, FRAP, phosphomolybdenum, and metal chelating assays. In addition, the bioactive compounds found in the extracts, such as total phenolics, flavonoids, and saponins, were determined. Cytotoxicity was assessed by using the CellTiter-Glo assay on HeLa and H1299 cancer cells. Results. The methanol extract of C. aucheri demonstrated the highest antioxidant activity as well as the highest phenolic, flavonoid, and saponin content. The high-performance liquid chromatography (HPLC) analysis showed that the major phenolic compounds in the extract were chlorogenic acid, (+)-catechin, rosmarinic acid, and rutin. The methanolic extract obtained from the aerial parts of C. aucheri was found to interfere with the viability of HeLa cells, with an IC50 value of 14.22 μg/mL being recorded. Conclusion. Our results showed that C. aucheri could be a good candidate as a novel and alternative natural antioxidant and antitumor source. |
| format | Article |
| id | doaj-art-e51466d6de8b4c27b8322b8b7db66116 |
| institution | Kabale University |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-e51466d6de8b4c27b8322b8b7db661162025-08-20T03:26:20ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202022-01-0179548849510.2298/VSP200114133O0042-84502000133OPhenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri ChoisyOzay Cennet0Mammadov Ramazan1Izmir Katip Celebi University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Izmir, Turkey + Muğla Sıtkı Koçman University, Faculty of Science, Department of Molecular Biology and Genetics, Muğla, TurkeyMuğla Sıtkı Koçman University, Faculty of Science, Department of Molecular Biology and Genetics, Muğla, TurkeyBackground/Aim. It is known that some members of the genus Convolvulus (C) L. are commonly used in Turkish folk medicine. These species are powerful in curing toothache and joint pains. Due to the limited information on the biological activities of C. aucheri, a species almost exclusively occurring in Turkey, we aimed to investigate the antioxidant and cytotoxic effects of three extracts obtained from the plant, as well as to characterize their phenolic profile. Methods. The anti-oxidant activity of the extracts was determined by using ABTS, NO, FRAP, phosphomolybdenum, and metal chelating assays. In addition, the bioactive compounds found in the extracts, such as total phenolics, flavonoids, and saponins, were determined. Cytotoxicity was assessed by using the CellTiter-Glo assay on HeLa and H1299 cancer cells. Results. The methanol extract of C. aucheri demonstrated the highest antioxidant activity as well as the highest phenolic, flavonoid, and saponin content. The high-performance liquid chromatography (HPLC) analysis showed that the major phenolic compounds in the extract were chlorogenic acid, (+)-catechin, rosmarinic acid, and rutin. The methanolic extract obtained from the aerial parts of C. aucheri was found to interfere with the viability of HeLa cells, with an IC50 value of 14.22 μg/mL being recorded. Conclusion. Our results showed that C. aucheri could be a good candidate as a novel and alternative natural antioxidant and antitumor source.http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502000133O.pdfantioxidantsconvolvuluschromatography, high-pressure liquidphytotherapyturkey |
| spellingShingle | Ozay Cennet Mammadov Ramazan Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy Vojnosanitetski Pregled antioxidants convolvulus chromatography, high-pressure liquid phytotherapy turkey |
| title | Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy |
| title_full | Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy |
| title_fullStr | Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy |
| title_full_unstemmed | Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy |
| title_short | Phenolic profile, antioxidant and antiproliferative activities of Convolvulus aucheri Choisy |
| title_sort | phenolic profile antioxidant and antiproliferative activities of convolvulus aucheri choisy |
| topic | antioxidants convolvulus chromatography, high-pressure liquid phytotherapy turkey |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502000133O.pdf |
| work_keys_str_mv | AT ozaycennet phenolicprofileantioxidantandantiproliferativeactivitiesofconvolvulusaucherichoisy AT mammadovramazan phenolicprofileantioxidantandantiproliferativeactivitiesofconvolvulusaucherichoisy |