Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus
Marcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in Xenopus laevis to reveal the novel roles of t...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2024-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/98277 |
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| author | Mohamed El Amri Abhay Pandit Gerhard Schlosser |
| author_facet | Mohamed El Amri Abhay Pandit Gerhard Schlosser |
| author_sort | Mohamed El Amri |
| collection | DOAJ |
| description | Marcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in Xenopus laevis to reveal the novel roles of these proteins in spinal cord development and regeneration. We show that Marcks and Marcksl1 have partly redundant functions and are required for normal neurite formation and proliferation of neuro-glial progenitors during embryonic spinal cord development and for its regeneration during tadpole stages. Rescue experiments in Marcks and Marcksl1 loss-of-function animals further suggested that some of the functions of Marcks and Marcksl1 in the spinal cord are mediated by phospholipid signaling. Taken together, these findings identify Marcks and Marcksl1 as critical new players in spinal cord development and regeneration and suggest new pathways to be targeted for therapeutic stimulation of spinal cord regeneration in human patients. |
| format | Article |
| id | doaj-art-e29e7034bf1446e78c8c4fcf65a0051d |
| institution | DOAJ |
| issn | 2050-084X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-e29e7034bf1446e78c8c4fcf65a0051d2025-08-20T02:50:29ZengeLife Sciences Publications LtdeLife2050-084X2024-12-011310.7554/eLife.98277Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in XenopusMohamed El Amri0https://orcid.org/0000-0002-7030-3192Abhay Pandit1Gerhard Schlosser2https://orcid.org/0000-0002-1300-1331School of Biological and Chemical Sciences, University of Galway, Galway, Ireland; Research Ireland Center for Medical Devices (CÚRAM), University of Galway, Galway, IrelandResearch Ireland Center for Medical Devices (CÚRAM), University of Galway, Galway, IrelandSchool of Biological and Chemical Sciences, University of Galway, Galway, IrelandMarcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in Xenopus laevis to reveal the novel roles of these proteins in spinal cord development and regeneration. We show that Marcks and Marcksl1 have partly redundant functions and are required for normal neurite formation and proliferation of neuro-glial progenitors during embryonic spinal cord development and for its regeneration during tadpole stages. Rescue experiments in Marcks and Marcksl1 loss-of-function animals further suggested that some of the functions of Marcks and Marcksl1 in the spinal cord are mediated by phospholipid signaling. Taken together, these findings identify Marcks and Marcksl1 as critical new players in spinal cord development and regeneration and suggest new pathways to be targeted for therapeutic stimulation of spinal cord regeneration in human patients.https://elifesciences.org/articles/98277regenerationspinal cordMarcksaxon outgrowthneurogenesisdevelopment |
| spellingShingle | Mohamed El Amri Abhay Pandit Gerhard Schlosser Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus eLife regeneration spinal cord Marcks axon outgrowth neurogenesis development |
| title | Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus |
| title_full | Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus |
| title_fullStr | Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus |
| title_full_unstemmed | Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus |
| title_short | Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus |
| title_sort | marcks and marcks like 1 proteins promote spinal cord development and regeneration in xenopus |
| topic | regeneration spinal cord Marcks axon outgrowth neurogenesis development |
| url | https://elifesciences.org/articles/98277 |
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