The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils

Background Forage production in tropical soils is primarily limited by nutrient deficiencies, especially nitrogen (N) and phosphorus (P). The use of phosphate rock by plants is limited by its low and slow P availability and microbial phosphate solubilization is the main mechanism for P bioavailabili...

Full description

Saved in:
Bibliographic Details
Main Authors: Alexandro Barbosa, Isbelia Reyes, Alexis Valery, Carlos Chacón Labrador, Oscar Martínez, Maximo F. Alonso
Format: Article
Language:English
Published: PeerJ Inc. 2024-12-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/18610.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846136724724383744
author Alexandro Barbosa
Isbelia Reyes
Alexis Valery
Carlos Chacón Labrador
Oscar Martínez
Maximo F. Alonso
author_facet Alexandro Barbosa
Isbelia Reyes
Alexis Valery
Carlos Chacón Labrador
Oscar Martínez
Maximo F. Alonso
author_sort Alexandro Barbosa
collection DOAJ
description Background Forage production in tropical soils is primarily limited by nutrient deficiencies, especially nitrogen (N) and phosphorus (P). The use of phosphate rock by plants is limited by its low and slow P availability and microbial phosphate solubilization is the main mechanism for P bioavailability in the soil-root system. The objectives of this study were (i) select a nitrogen-fixing bacteria which could be used as a co-inoculant with the Penicillium rugulosum IR94MF1 phosphate-solubilizing fungus and (ii) evaluate under field conditions the effect of inoculation combined with phosphate rock (PR) application on yield and nutrient absorption of a Urochloa decumbens pasture which was previously established in a low-fertility, acidic soil. Methods Various laboratory and greenhouse tests allowed for the selection of Enterobacter cloacae C17 as the co-inoculant bacteria with the IR94MF1 fungus. Later, under field conditions, a factorial, completely randomized block design was used to evaluate the inoculation with the IR94MF1 fungus, the IR94MF1+C17 co-inoculation, and a non-inoculated control. Two levels of fertilization with PR treatment (0 kg/ha and 200 kg/ha P2O5) were applied to each. Results During five consecutive harvests it was observed that the addition of biofertilizers significantly increased (p < 0.05) the herbage mass and N and P assimilation compared to the non-inoculated control. However, no statistically significant differences were observed for the PR application as P source. Conclusion P. rugulosum IR94MF1 is capable of solubilizing and accumulating P from the phosphate rock, making it available for plants growing in acid soils with low N content. These inoculants represent a good option as biofertilizers for tropical grasses already established in acidic soils with low N content.
format Article
id doaj-art-d9cf268e3ffe479e833803ed9f22601d
institution Kabale University
issn 2167-8359
language English
publishDate 2024-12-01
publisher PeerJ Inc.
record_format Article
series PeerJ
spelling doaj-art-d9cf268e3ffe479e833803ed9f22601d2024-12-08T15:05:15ZengPeerJ Inc.PeerJ2167-83592024-12-0112e1861010.7717/peerj.18610The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soilsAlexandro Barbosa0Isbelia Reyes1Alexis Valery2Carlos Chacón Labrador3Oscar Martínez4Maximo F. Alonso5Escuela de Graduados, Facultad de Ciencias Agrarias y Alimentarias, Universidad Austral de Chile, Valdivia, Los Ríos, ChileDecanato de Investigación, Universidad Nacional Experimental del Táchira, San Cristobal, Tachira, VenezuelaDecanato de Investigación, Universidad Nacional Experimental del Táchira, San Cristobal, Tachira, VenezuelaDepartamento de Ingeniería Agronómica, Universidad Nacional Experimental del Táchira, San Cristobal, Tachira, VenezuelaInstituto de Microbiología y Bioquímica, Universidad Austral de Chile, Valdivia, Los Ríos, ChileInstituto de Producción Animal, Facultad de Ciencias Agrarias y Alimentarias, Universidad Austral de Chile, Valdivia, Los Ríos, ChileBackground Forage production in tropical soils is primarily limited by nutrient deficiencies, especially nitrogen (N) and phosphorus (P). The use of phosphate rock by plants is limited by its low and slow P availability and microbial phosphate solubilization is the main mechanism for P bioavailability in the soil-root system. The objectives of this study were (i) select a nitrogen-fixing bacteria which could be used as a co-inoculant with the Penicillium rugulosum IR94MF1 phosphate-solubilizing fungus and (ii) evaluate under field conditions the effect of inoculation combined with phosphate rock (PR) application on yield and nutrient absorption of a Urochloa decumbens pasture which was previously established in a low-fertility, acidic soil. Methods Various laboratory and greenhouse tests allowed for the selection of Enterobacter cloacae C17 as the co-inoculant bacteria with the IR94MF1 fungus. Later, under field conditions, a factorial, completely randomized block design was used to evaluate the inoculation with the IR94MF1 fungus, the IR94MF1+C17 co-inoculation, and a non-inoculated control. Two levels of fertilization with PR treatment (0 kg/ha and 200 kg/ha P2O5) were applied to each. Results During five consecutive harvests it was observed that the addition of biofertilizers significantly increased (p < 0.05) the herbage mass and N and P assimilation compared to the non-inoculated control. However, no statistically significant differences were observed for the PR application as P source. Conclusion P. rugulosum IR94MF1 is capable of solubilizing and accumulating P from the phosphate rock, making it available for plants growing in acid soils with low N content. These inoculants represent a good option as biofertilizers for tropical grasses already established in acidic soils with low N content.https://peerj.com/articles/18610.pdfSolubilization of phosphatesPenicillium rugulosumEnterobacter cloacaeMicrobial co-inoculation
spellingShingle Alexandro Barbosa
Isbelia Reyes
Alexis Valery
Carlos Chacón Labrador
Oscar Martínez
Maximo F. Alonso
The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils
PeerJ
Solubilization of phosphates
Penicillium rugulosum
Enterobacter cloacae
Microbial co-inoculation
title The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils
title_full The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils
title_fullStr The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils
title_full_unstemmed The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils
title_short The use of phosphate rock and plant growth promoting microorganisms for the management of Urochloa decumbens (Stapf.) R.D. Webster in acidic soils
title_sort use of phosphate rock and plant growth promoting microorganisms for the management of urochloa decumbens stapf r d webster in acidic soils
topic Solubilization of phosphates
Penicillium rugulosum
Enterobacter cloacae
Microbial co-inoculation
url https://peerj.com/articles/18610.pdf
work_keys_str_mv AT alexandrobarbosa theuseofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT isbeliareyes theuseofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT alexisvalery theuseofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT carloschaconlabrador theuseofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT oscarmartinez theuseofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT maximofalonso theuseofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT alexandrobarbosa useofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT isbeliareyes useofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT alexisvalery useofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT carloschaconlabrador useofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT oscarmartinez useofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils
AT maximofalonso useofphosphaterockandplantgrowthpromotingmicroorganismsforthemanagementofurochloadecumbensstapfrdwebsterinacidicsoils