Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis
Background. Icariin (ICA) is an active compound extracted from Epimedium brevicornum Maxim. Previous reports have shown that icariin has a clinically significant therapeutic effect on rheumatoid arthritis. However, little is known about the mechanism by which icariin inhibits cartilage and bone degr...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9529630 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849472870579699712 |
|---|---|
| author | Chen Chao Wei Dai Qi Ping Fan Tian You Chen Yong Qiang Che Tao |
| author_facet | Chen Chao Wei Dai Qi Ping Fan Tian You Chen Yong Qiang Che Tao |
| author_sort | Chen Chao Wei |
| collection | DOAJ |
| description | Background. Icariin (ICA) is an active compound extracted from Epimedium brevicornum Maxim. Previous reports have shown that icariin has a clinically significant therapeutic effect on rheumatoid arthritis. However, little is known about the mechanism by which icariin inhibits cartilage and bone degradation. Methods. New Zealand rabbits were immunized with antigen-induced arthritis (AIA) and treated with icariin. Joint tissues from rabbits were studied by histological analysis, transmission electron microscopy (TEM), and micro-CT. The expression levels of receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) in joint tissues were determined using immunohistochemistry and real-time PCR analysis. Results. Histological analysis and TEM sections of cartilage in the ICA treated group showed a low level of chondrocyte destruction. Micro-CT analysis showed that the bone mineral density value and bone structural level in ICA treated rabbits were significantly higher compared with those in the AIA group. Immunohistochemistry and real-time PCR analysis showed that icariin treatment reduced RANKL expression and enhanced OPG expression levels, as compared to the AIA group. Conclusion. These data indicate that ICA suppresses articular bone loss and prevents joint destruction. This study also determined that ICA regulated articular bone loss in part by regulating RANKL and OPG expression. |
| format | Article |
| id | doaj-art-d9bb95ed60b64f4da751e5d7e104c52a |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d9bb95ed60b64f4da751e5d7e104c52a2025-08-20T03:24:22ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/95296309529630Icariin Prevents Cartilage and Bone Degradation in Experimental Models of ArthritisChen Chao Wei0Dai Qi Ping1Fan Tian You2Chen Yong Qiang3Che Tao4Department of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of TCM, Shanghai 200071, ChinaDepartment of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of TCM, Shanghai 200071, ChinaDepartment of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of TCM, Shanghai 200071, ChinaDepartment of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of TCM, Shanghai 200071, ChinaDepartment of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of TCM, Shanghai 200071, ChinaBackground. Icariin (ICA) is an active compound extracted from Epimedium brevicornum Maxim. Previous reports have shown that icariin has a clinically significant therapeutic effect on rheumatoid arthritis. However, little is known about the mechanism by which icariin inhibits cartilage and bone degradation. Methods. New Zealand rabbits were immunized with antigen-induced arthritis (AIA) and treated with icariin. Joint tissues from rabbits were studied by histological analysis, transmission electron microscopy (TEM), and micro-CT. The expression levels of receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) in joint tissues were determined using immunohistochemistry and real-time PCR analysis. Results. Histological analysis and TEM sections of cartilage in the ICA treated group showed a low level of chondrocyte destruction. Micro-CT analysis showed that the bone mineral density value and bone structural level in ICA treated rabbits were significantly higher compared with those in the AIA group. Immunohistochemistry and real-time PCR analysis showed that icariin treatment reduced RANKL expression and enhanced OPG expression levels, as compared to the AIA group. Conclusion. These data indicate that ICA suppresses articular bone loss and prevents joint destruction. This study also determined that ICA regulated articular bone loss in part by regulating RANKL and OPG expression.http://dx.doi.org/10.1155/2016/9529630 |
| spellingShingle | Chen Chao Wei Dai Qi Ping Fan Tian You Chen Yong Qiang Che Tao Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis Mediators of Inflammation |
| title | Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis |
| title_full | Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis |
| title_fullStr | Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis |
| title_full_unstemmed | Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis |
| title_short | Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis |
| title_sort | icariin prevents cartilage and bone degradation in experimental models of arthritis |
| url | http://dx.doi.org/10.1155/2016/9529630 |
| work_keys_str_mv | AT chenchaowei icariinpreventscartilageandbonedegradationinexperimentalmodelsofarthritis AT daiqiping icariinpreventscartilageandbonedegradationinexperimentalmodelsofarthritis AT fantianyou icariinpreventscartilageandbonedegradationinexperimentalmodelsofarthritis AT chenyongqiang icariinpreventscartilageandbonedegradationinexperimentalmodelsofarthritis AT chetao icariinpreventscartilageandbonedegradationinexperimentalmodelsofarthritis |