Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals
Background: Klebsiella pneumoniae has two different pathotypes: hypervirulent Klebsiella pneumoniae (hvkp) and classical Klebsiella pneumoniae (ckp), hvkp is more potent than ckp causing vast types of severe disseminated infections. This cross sectional study done to detect frequency of hvkp versus...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zagazig University, Faculty of Medicine
2024-05-01
|
| Series: | Microbes and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mid.journals.ekb.eg/article_348026_af231740fd125d3cf6eb8fb89f57c165.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846113242607255552 |
|---|---|
| author | Nesma Mohamed Hassan Ashgan Badawy Dawood Mona mohamed Shereef A Sayed Dina Mohamed |
| author_facet | Nesma Mohamed Hassan Ashgan Badawy Dawood Mona mohamed Shereef A Sayed Dina Mohamed |
| author_sort | Nesma Mohamed Hassan |
| collection | DOAJ |
| description | Background: Klebsiella pneumoniae has two different pathotypes: hypervirulent Klebsiella pneumoniae (hvkp) and classical Klebsiella pneumoniae (ckp), hvkp is more potent than ckp causing vast types of severe disseminated infections. This cross sectional study done to detect frequency of hvkp versus ckp , their antibiotic resistance and risk factors associated with their infections. Methods: Samples were collected from admitted patients cultured on MacConkey agar, pink colonies were confirmed to be Klebsiella pneumonia and sensitivity were done by Vitek 2. String test was done for hypermucoviscosity and PCR to detect virulence genes. Results: 100 samples were collected from 2020 to 2023, 64 of them revealed hvkp which were isolated from old age with mean± SD of 62.7±20.5, most of isolates were from ICU from pneumonia patients. There was a significant difference in ceftriaxone, cefoxitin, gentamycin and tobramycin resistance between hvkp, ckp with p value 0.03, 0.04 ,0.01 and 0.02 respectively, 24% of hvkp strains were ESBL producers. Fourteen (14%) were pandrug resistant, 14(14%) were extremely drug resistant and 36(36%) were multidrug resistant. iucA was the most frequent virulence gene 62% followed by fimH (42%). Diabetes mellitus, hypertension and cardiovascular disease were the most significant risk factors associated with hvkp infection. Conclusion: hvkp is a serious pathogen that utilizes a battery of virulence factors for survival and pathogenesis, also has an ability to acquire drug resistance through transfer of genetic material, so we have to shed light on new strategies to improve diagnosis, treatment and prevention of hvkp causing infections. |
| format | Article |
| id | doaj-art-cfc50df32994409ab1e2feec1691ebf9 |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj-art-cfc50df32994409ab1e2feec1691ebf92024-12-21T21:02:47ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-05-015273874810.21608/mid.2024.272832.1823348026Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University HospitalsNesma Mohamed Hassan0Ashgan Badawy Dawood1Mona mohamed2Shereef A Sayed3Dina Mohamed4Medical microbiology and Immunology Department, Faculty of Medicine, Sohag University, EgyptMedical microbiology and Immunology Department, Faculty of Medicine, Sohag University, EgyptMedical microbiology and Immunology Department, Faculty of Medicine, Sohag University, EgyptClinical Pathology Department, Faculty of medicine, Sohag university, EgyptMedical microbiology and Immunology Department, Faculty of Medicine, Sohag University, EgyptBackground: Klebsiella pneumoniae has two different pathotypes: hypervirulent Klebsiella pneumoniae (hvkp) and classical Klebsiella pneumoniae (ckp), hvkp is more potent than ckp causing vast types of severe disseminated infections. This cross sectional study done to detect frequency of hvkp versus ckp , their antibiotic resistance and risk factors associated with their infections. Methods: Samples were collected from admitted patients cultured on MacConkey agar, pink colonies were confirmed to be Klebsiella pneumonia and sensitivity were done by Vitek 2. String test was done for hypermucoviscosity and PCR to detect virulence genes. Results: 100 samples were collected from 2020 to 2023, 64 of them revealed hvkp which were isolated from old age with mean± SD of 62.7±20.5, most of isolates were from ICU from pneumonia patients. There was a significant difference in ceftriaxone, cefoxitin, gentamycin and tobramycin resistance between hvkp, ckp with p value 0.03, 0.04 ,0.01 and 0.02 respectively, 24% of hvkp strains were ESBL producers. Fourteen (14%) were pandrug resistant, 14(14%) were extremely drug resistant and 36(36%) were multidrug resistant. iucA was the most frequent virulence gene 62% followed by fimH (42%). Diabetes mellitus, hypertension and cardiovascular disease were the most significant risk factors associated with hvkp infection. Conclusion: hvkp is a serious pathogen that utilizes a battery of virulence factors for survival and pathogenesis, also has an ability to acquire drug resistance through transfer of genetic material, so we have to shed light on new strategies to improve diagnosis, treatment and prevention of hvkp causing infections.https://mid.journals.ekb.eg/article_348026_af231740fd125d3cf6eb8fb89f57c165.pdfhvkprisk factorsantibiotic resistance |
| spellingShingle | Nesma Mohamed Hassan Ashgan Badawy Dawood Mona mohamed Shereef A Sayed Dina Mohamed Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals Microbes and Infectious Diseases hvkp risk factors antibiotic resistance |
| title | Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals |
| title_full | Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals |
| title_fullStr | Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals |
| title_full_unstemmed | Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals |
| title_short | Distribution, characterization and antibiotic resistance of hypervirulent Klebsiella Pneumoniae (hvKp) strains versus classical strains(CKp) causing healthcare associated infections in Sohag University Hospitals |
| title_sort | distribution characterization and antibiotic resistance of hypervirulent klebsiella pneumoniae hvkp strains versus classical strains ckp causing healthcare associated infections in sohag university hospitals |
| topic | hvkp risk factors antibiotic resistance |
| url | https://mid.journals.ekb.eg/article_348026_af231740fd125d3cf6eb8fb89f57c165.pdf |
| work_keys_str_mv | AT nesmamohamedhassan distributioncharacterizationandantibioticresistanceofhypervirulentklebsiellapneumoniaehvkpstrainsversusclassicalstrainsckpcausinghealthcareassociatedinfectionsinsohaguniversityhospitals AT ashganbadawydawood distributioncharacterizationandantibioticresistanceofhypervirulentklebsiellapneumoniaehvkpstrainsversusclassicalstrainsckpcausinghealthcareassociatedinfectionsinsohaguniversityhospitals AT monamohamed distributioncharacterizationandantibioticresistanceofhypervirulentklebsiellapneumoniaehvkpstrainsversusclassicalstrainsckpcausinghealthcareassociatedinfectionsinsohaguniversityhospitals AT shereefasayed distributioncharacterizationandantibioticresistanceofhypervirulentklebsiellapneumoniaehvkpstrainsversusclassicalstrainsckpcausinghealthcareassociatedinfectionsinsohaguniversityhospitals AT dinamohamed distributioncharacterizationandantibioticresistanceofhypervirulentklebsiellapneumoniaehvkpstrainsversusclassicalstrainsckpcausinghealthcareassociatedinfectionsinsohaguniversityhospitals |