Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation
<i>Rosa hybrida</i> is one the most commonly cultivated ornamental plant of economic importance and faces major challenges under heat stress. Melatonin has been widely shown to regulate plant stress response; however, the exact mechanism involved in heat stress in <i>R. hybrida<...
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2024-12-01
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| author | Chenyang Li Zhiyin Ding Zipeng Cai Yongying Ruan Peitao Lü Yang Liu |
| author_facet | Chenyang Li Zhiyin Ding Zipeng Cai Yongying Ruan Peitao Lü Yang Liu |
| author_sort | Chenyang Li |
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| description | <i>Rosa hybrida</i> is one the most commonly cultivated ornamental plant of economic importance and faces major challenges under heat stress. Melatonin has been widely shown to regulate plant stress response; however, the exact mechanism involved in heat stress in <i>R. hybrida</i> has yet to be determined. Here, we observed that <i>R. hybrida</i> in vitro plantlets supplemented with melatonin in the culture medium exhibited higher chlorophyll content, relative ion leakage, and fresh weight after 12 d of high-temperature treatment; the optimal concentration was established at 5 mg/L. Using molecular and biochemical techniques, we explored the roles of a melatonin synthase gene <i>RhCOMT1</i>, which expression was influenced by heat stress and melatonin. RhCOMT1 was located in the nuclear-cytoplasmic under ambient conditions, while heat stress translocated the distribution of RhCOMT1 to chloroplasts. Overexpression of <i>RhCOMT1</i> in rose petal enhanced thermotolerance, and silencing of <i>RhCOMT1</i> reduced thermotolerance via affect H<sub>2</sub>O<sub>2</sub> content and relative ion leakage. These findings collectively emphasize the pivotal role of melatonin in enhancing thermotolerance to <i>R. hybrida</i> by alleviation of oxidative stress, through modulation of <i>RhCOMT1</i> expression and location. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-cd3133f933a64d13897c3af56189973a2025-01-10T13:19:32ZengMDPI AGPlants2223-77472024-12-011412910.3390/plants14010029Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> ModulationChenyang Li0Zhiyin Ding1Zipeng Cai2Yongying Ruan3Peitao Lü4Yang Liu5Center for Plant Metabolomics, Haixia Institute of Science and Technology, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Architectural Engineering, Shenzhen Polytechnic University, Shenzhen 518055, ChinaCollege of Architectural Engineering, Shenzhen Polytechnic University, Shenzhen 518055, ChinaCollege of Architectural Engineering, Shenzhen Polytechnic University, Shenzhen 518055, ChinaNational Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, ChinaCollege of Architectural Engineering, Shenzhen Polytechnic University, Shenzhen 518055, China<i>Rosa hybrida</i> is one the most commonly cultivated ornamental plant of economic importance and faces major challenges under heat stress. Melatonin has been widely shown to regulate plant stress response; however, the exact mechanism involved in heat stress in <i>R. hybrida</i> has yet to be determined. Here, we observed that <i>R. hybrida</i> in vitro plantlets supplemented with melatonin in the culture medium exhibited higher chlorophyll content, relative ion leakage, and fresh weight after 12 d of high-temperature treatment; the optimal concentration was established at 5 mg/L. Using molecular and biochemical techniques, we explored the roles of a melatonin synthase gene <i>RhCOMT1</i>, which expression was influenced by heat stress and melatonin. RhCOMT1 was located in the nuclear-cytoplasmic under ambient conditions, while heat stress translocated the distribution of RhCOMT1 to chloroplasts. Overexpression of <i>RhCOMT1</i> in rose petal enhanced thermotolerance, and silencing of <i>RhCOMT1</i> reduced thermotolerance via affect H<sub>2</sub>O<sub>2</sub> content and relative ion leakage. These findings collectively emphasize the pivotal role of melatonin in enhancing thermotolerance to <i>R. hybrida</i> by alleviation of oxidative stress, through modulation of <i>RhCOMT1</i> expression and location.https://www.mdpi.com/2223-7747/14/1/29<i>Rosa hybrida</i>heat stressCOMTmelatoninprotein translocation |
| spellingShingle | Chenyang Li Zhiyin Ding Zipeng Cai Yongying Ruan Peitao Lü Yang Liu Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation Plants <i>Rosa hybrida</i> heat stress COMT melatonin protein translocation |
| title | Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation |
| title_full | Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation |
| title_fullStr | Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation |
| title_full_unstemmed | Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation |
| title_short | Exogenous Melatonin Boosts Heat Tolerance in <i>Rosa hybrida</i> via <i>RhCOMT1</i> Modulation |
| title_sort | exogenous melatonin boosts heat tolerance in i rosa hybrida i via i rhcomt1 i modulation |
| topic | <i>Rosa hybrida</i> heat stress COMT melatonin protein translocation |
| url | https://www.mdpi.com/2223-7747/14/1/29 |
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