Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship
The spectrum-effect relationship between chemical fingerprints and antioxidant activities was established to evaluate the quality of Eucommiae Folium (EF) extracts and discover potential efficacy-associated quality markers. Characteristic fingerprints of different batches of EF extracts were generat...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-09-01
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| Series: | International Journal of Food Properties |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2023.2209703 |
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| author | Weiwei Zhou Xuefeng Wang Juan Yang Jiahong Dou Yuelong Sun Mengjie Zhang Jun Li Xiumei Li |
| author_facet | Weiwei Zhou Xuefeng Wang Juan Yang Jiahong Dou Yuelong Sun Mengjie Zhang Jun Li Xiumei Li |
| author_sort | Weiwei Zhou |
| collection | DOAJ |
| description | The spectrum-effect relationship between chemical fingerprints and antioxidant activities was established to evaluate the quality of Eucommiae Folium (EF) extracts and discover potential efficacy-associated quality markers. Characteristic fingerprints of different batches of EF extracts were generated by high-performance liquid chromatography (HPLC), and a similarity analysis was performed by hierarchical cluster analysis (HCA). Then the total antioxidant capacity assay kit with ferric reducing antioxidant assay (FRAP) method was used to determine antioxidant activity. The spectrum-effect relationship was established to identify the chemical constituents that impart antioxidant activities of Eucommiae Folium (EF) extracts using partial least squares regression (PLSR). HPLC fingerprints of EF extracts were established with the optimized chromatographic conditions: the mobile phase consisted 0.1% phosphoric acid -water (A) and acetonitrile (B), the detection wavelength was 326 nm, the column temperature was 30°C, and the flow rate was 0.8 mL/min. The correlation coefficient of similarity between 12 batches of extracts was ≥0.995. Compounds corresponding peaks P1, P2, P6 (chlorogenic acid), and P8 were closely related to the stable antioxidant activities of EF extracts, and could be selected as potential quality markers. An agile and efficient method that can ensure the intra-assay stability of biological activity of EF extracts and discover efficacy-associated quality markers was successfully built based on spectrum-effect relationship. |
| format | Article |
| id | doaj-art-ccf8b08935e34258bebdd517be13a374 |
| institution | Kabale University |
| issn | 1094-2912 1532-2386 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj-art-ccf8b08935e34258bebdd517be13a3742025-01-02T10:41:35ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862023-09-012611264127610.1080/10942912.2023.2209703Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationshipWeiwei Zhou0Xuefeng Wang1Juan Yang2Jiahong Dou3Yuelong Sun4Mengjie Zhang5Jun Li6Xiumei Li7Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of pharmacy, Harbin University of Commerce, Harbin, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education & Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, ChinaThe spectrum-effect relationship between chemical fingerprints and antioxidant activities was established to evaluate the quality of Eucommiae Folium (EF) extracts and discover potential efficacy-associated quality markers. Characteristic fingerprints of different batches of EF extracts were generated by high-performance liquid chromatography (HPLC), and a similarity analysis was performed by hierarchical cluster analysis (HCA). Then the total antioxidant capacity assay kit with ferric reducing antioxidant assay (FRAP) method was used to determine antioxidant activity. The spectrum-effect relationship was established to identify the chemical constituents that impart antioxidant activities of Eucommiae Folium (EF) extracts using partial least squares regression (PLSR). HPLC fingerprints of EF extracts were established with the optimized chromatographic conditions: the mobile phase consisted 0.1% phosphoric acid -water (A) and acetonitrile (B), the detection wavelength was 326 nm, the column temperature was 30°C, and the flow rate was 0.8 mL/min. The correlation coefficient of similarity between 12 batches of extracts was ≥0.995. Compounds corresponding peaks P1, P2, P6 (chlorogenic acid), and P8 were closely related to the stable antioxidant activities of EF extracts, and could be selected as potential quality markers. An agile and efficient method that can ensure the intra-assay stability of biological activity of EF extracts and discover efficacy-associated quality markers was successfully built based on spectrum-effect relationship.https://www.tandfonline.com/doi/10.1080/10942912.2023.2209703EF extractsSpectrum-effect relationshipAntioxidant activityQuality evaluationQuality markers |
| spellingShingle | Weiwei Zhou Xuefeng Wang Juan Yang Jiahong Dou Yuelong Sun Mengjie Zhang Jun Li Xiumei Li Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship International Journal of Food Properties EF extracts Spectrum-effect relationship Antioxidant activity Quality evaluation Quality markers |
| title | Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship |
| title_full | Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship |
| title_fullStr | Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship |
| title_full_unstemmed | Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship |
| title_short | Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship |
| title_sort | quality evaluation of eucommiae folium extracts and discovery of efficacy associated quality markers based on spectrum effect relationship |
| topic | EF extracts Spectrum-effect relationship Antioxidant activity Quality evaluation Quality markers |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2023.2209703 |
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