Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects
Coptidis Rhizoma (CR), a traditional Chinese medicine, has extensive pharmacological activity because it is rich in isoquinoline alkaloids. Nonetheless, the limited solubility of these alkaloids presents a significant challenge, hindering the full realization of CR’s therapeutic potential through co...
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Elsevier
2025-08-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725001750 |
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| author | Huiwen Wang Haixin Peng Wenwen Ding Zhiyun Zhang Yadan Zheng Sikai Chen Xueying Chen Qianwei Qu Yanyan Liu Yanhua Li |
| author_facet | Huiwen Wang Haixin Peng Wenwen Ding Zhiyun Zhang Yadan Zheng Sikai Chen Xueying Chen Qianwei Qu Yanyan Liu Yanhua Li |
| author_sort | Huiwen Wang |
| collection | DOAJ |
| description | Coptidis Rhizoma (CR), a traditional Chinese medicine, has extensive pharmacological activity because it is rich in isoquinoline alkaloids. Nonetheless, the limited solubility of these alkaloids presents a significant challenge, hindering the full realization of CR’s therapeutic potential through conventional extraction methods. To address this issue and enhance the solubility of the target compounds while optimizing extraction efficiency, this study employed an environmentally sustainable extraction technique, Ultrasound-Enzyme-Trehalose Lipid (UET), to synergistically extract five key alkaloids from CR. Based on the results of single-factor experiments, Box-Behnken design (BBD) was employed to optimize the selected model variables. The optimal extraction conditions were determined: extraction pH of 4.3, extraction temperature of 40 ℃, solid–liquid ratio of 1:27 g/mL, and ultrasonication time of 48 min. UET extracts were compared with other extracts, and it was proved that UET extracts had higher alkaloid extraction rate, comprehensive evaluation value (CEV) and lower energy consumption and CO2 emission. Then scanning electron microscopy (SEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to explore the extraction mechanism of UET extraction. In addition, UET extraction has more excellent anti-inflammatory activity. The establishment of UET method of CR provides a method reference for green and efficient extraction of alkaloid components from natural drugs. |
| format | Article |
| id | doaj-art-c7a4b903e7c9462e9505c19e5c0703bf |
| institution | DOAJ |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-c7a4b903e7c9462e9505c19e5c0703bf2025-08-20T02:39:39ZengElsevierUltrasonics Sonochemistry1350-41772025-08-0111910739610.1016/j.ultsonch.2025.107396Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effectsHuiwen Wang0Haixin Peng1Wenwen Ding2Zhiyun Zhang3Yadan Zheng4Sikai Chen5Xueying Chen6Qianwei Qu7Yanyan Liu8Yanhua Li9College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; The Laboratory of Molecular Nutrition and Immunity, College of Animal Science and Technol, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.Coptidis Rhizoma (CR), a traditional Chinese medicine, has extensive pharmacological activity because it is rich in isoquinoline alkaloids. Nonetheless, the limited solubility of these alkaloids presents a significant challenge, hindering the full realization of CR’s therapeutic potential through conventional extraction methods. To address this issue and enhance the solubility of the target compounds while optimizing extraction efficiency, this study employed an environmentally sustainable extraction technique, Ultrasound-Enzyme-Trehalose Lipid (UET), to synergistically extract five key alkaloids from CR. Based on the results of single-factor experiments, Box-Behnken design (BBD) was employed to optimize the selected model variables. The optimal extraction conditions were determined: extraction pH of 4.3, extraction temperature of 40 ℃, solid–liquid ratio of 1:27 g/mL, and ultrasonication time of 48 min. UET extracts were compared with other extracts, and it was proved that UET extracts had higher alkaloid extraction rate, comprehensive evaluation value (CEV) and lower energy consumption and CO2 emission. Then scanning electron microscopy (SEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to explore the extraction mechanism of UET extraction. In addition, UET extraction has more excellent anti-inflammatory activity. The establishment of UET method of CR provides a method reference for green and efficient extraction of alkaloid components from natural drugs.http://www.sciencedirect.com/science/article/pii/S1350417725001750Trehalose lipid extractionAlkaloid compoundsComprehensive evaluation of valueEnergy and CO2 assessmentAnti-inflammatory activity evaluationGreen extraction |
| spellingShingle | Huiwen Wang Haixin Peng Wenwen Ding Zhiyun Zhang Yadan Zheng Sikai Chen Xueying Chen Qianwei Qu Yanyan Liu Yanhua Li Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects Ultrasonics Sonochemistry Trehalose lipid extraction Alkaloid compounds Comprehensive evaluation of value Energy and CO2 assessment Anti-inflammatory activity evaluation Green extraction |
| title | Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects |
| title_full | Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects |
| title_fullStr | Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects |
| title_full_unstemmed | Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects |
| title_short | Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects |
| title_sort | optimization of ultrasound enzyme assisted trehalose lipid extraction of coptis alkaloids and evaluation of its anti inflammatory effects |
| topic | Trehalose lipid extraction Alkaloid compounds Comprehensive evaluation of value Energy and CO2 assessment Anti-inflammatory activity evaluation Green extraction |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725001750 |
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