Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile
Microalgae are photosynthetic organisms with rapid growth and high biochemical diversity, capable of thriving in a variety of environments. Among them, dinoflagellates, particularly symbiotic species like <i>Durusdinium glynnii</i>, have gained attention due to their potential for biotec...
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2025-06-01
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| author | Pedro Rodrigues de Sena Maria Eunice S. S. Lira Deyvid Willame S. Oliveira Barbara de Cassia S. Brandão Jessika L. de Abreu Watson Arantes Gama Evando S. Araújo Giustino Tribuzi Alfredo O. Gálvez Carlos Yure B. Oliveira |
| author_facet | Pedro Rodrigues de Sena Maria Eunice S. S. Lira Deyvid Willame S. Oliveira Barbara de Cassia S. Brandão Jessika L. de Abreu Watson Arantes Gama Evando S. Araújo Giustino Tribuzi Alfredo O. Gálvez Carlos Yure B. Oliveira |
| author_sort | Pedro Rodrigues de Sena |
| collection | DOAJ |
| description | Microalgae are photosynthetic organisms with rapid growth and high biochemical diversity, capable of thriving in a variety of environments. Among them, dinoflagellates, particularly symbiotic species like <i>Durusdinium glynnii</i>, have gained attention due to their potential for biotechnological applications, especially in the production of valuable fatty acids. However, the delicate cultivation of dinoflagellates remains a challenge due to their sensitivity to shear stress and complex morphology. In this study, we evaluated the influence of inoculum percentage (10%, 25%, and 50%) on the growth performance and fatty acid profile of <i>D. glynnii</i> during a scale-up process from test tubes to a pilot-scale photobioreactor. Higher inoculum concentrations (50%) promoted faster acclimatization, higher specific growth rates (µ<sub>max</sub>), and greater final biomass densities, optimizing the cultivation process. Meanwhile, lower inoculum concentrations (10%) favored the accumulation of polyunsaturated fatty acids, particularly DHA (C22:6n3), indicating a trade-off between biomass productivity and fatty acid biosynthesis. Overall, <i>D. glynnii</i> demonstrated robust adaptability, reinforcing its potential as a sustainable source of bioactive compounds. Further studies focusing on cellular and metabolic pathways are needed to better elucidate the mechanisms underlying lipid production and growth in this promising species. |
| format | Article |
| id | doaj-art-c489d609bc6243b091d9538409abbd3e |
| institution | Kabale University |
| issn | 2673-9410 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Phycology |
| spelling | doaj-art-c489d609bc6243b091d9538409abbd3e2025-08-20T03:29:35ZengMDPI AGPhycology2673-94102025-06-01522110.3390/phycology5020021Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid ProfilePedro Rodrigues de Sena0Maria Eunice S. S. Lira1Deyvid Willame S. Oliveira2Barbara de Cassia S. Brandão3Jessika L. de Abreu4Watson Arantes Gama5Evando S. Araújo6Giustino Tribuzi7Alfredo O. Gálvez8Carlos Yure B. Oliveira9Department of Fisheries and Aquaculture, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilDepartment of Fisheries and Aquaculture, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilDepartment of Fisheries and Aquaculture, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilDepartment of Fisheries and Aquaculture, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilDepartment of Fisheries and Aquaculture, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilDepartment of Biology, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilResearch Group on Electrospinning and Nanotechnology Applications, Department of Materials Science, Federal University of San Francisco Valley, Juazeiro 48902-300, BA, BrazilDepartment of Food Science and Technology, Federal University of Santa Catarina, Florianopólis 88034-001, SC, BrazilDepartment of Fisheries and Aquaculture, Federal Rural University of Pernambuco, Recife 52171-900, PE, BrazilDepartment of Botany, Federal University of Santa Catarina, Florianópolis 88040-900, SC, BrazilMicroalgae are photosynthetic organisms with rapid growth and high biochemical diversity, capable of thriving in a variety of environments. Among them, dinoflagellates, particularly symbiotic species like <i>Durusdinium glynnii</i>, have gained attention due to their potential for biotechnological applications, especially in the production of valuable fatty acids. However, the delicate cultivation of dinoflagellates remains a challenge due to their sensitivity to shear stress and complex morphology. In this study, we evaluated the influence of inoculum percentage (10%, 25%, and 50%) on the growth performance and fatty acid profile of <i>D. glynnii</i> during a scale-up process from test tubes to a pilot-scale photobioreactor. Higher inoculum concentrations (50%) promoted faster acclimatization, higher specific growth rates (µ<sub>max</sub>), and greater final biomass densities, optimizing the cultivation process. Meanwhile, lower inoculum concentrations (10%) favored the accumulation of polyunsaturated fatty acids, particularly DHA (C22:6n3), indicating a trade-off between biomass productivity and fatty acid biosynthesis. Overall, <i>D. glynnii</i> demonstrated robust adaptability, reinforcing its potential as a sustainable source of bioactive compounds. Further studies focusing on cellular and metabolic pathways are needed to better elucidate the mechanisms underlying lipid production and growth in this promising species.https://www.mdpi.com/2673-9410/5/2/21dinoflagellateendosymbiontsfatty acidsinoculation strategy |
| spellingShingle | Pedro Rodrigues de Sena Maria Eunice S. S. Lira Deyvid Willame S. Oliveira Barbara de Cassia S. Brandão Jessika L. de Abreu Watson Arantes Gama Evando S. Araújo Giustino Tribuzi Alfredo O. Gálvez Carlos Yure B. Oliveira Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile Phycology dinoflagellate endosymbionts fatty acids inoculation strategy |
| title | Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile |
| title_full | Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile |
| title_fullStr | Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile |
| title_full_unstemmed | Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile |
| title_short | Scale-Up Cultivation of the Dinoflagellate <i>Durusdinium glynnii</i> Under Varying Inoculum Percentages: Effects on Growth Performance and Fatty Acid Profile |
| title_sort | scale up cultivation of the dinoflagellate i durusdinium glynnii i under varying inoculum percentages effects on growth performance and fatty acid profile |
| topic | dinoflagellate endosymbionts fatty acids inoculation strategy |
| url | https://www.mdpi.com/2673-9410/5/2/21 |
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