Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses
Abstract The SHI RELATED SEQUENCE (SRS) family plays a vital role in the development of multiple plant organs such as floral meristem determinacy, organ morphogenesis, and signal transduction. Nevertheless, there is little understanding of the biological significance of tomato SRS family at this poi...
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2023-10-01
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| Online Access: | https://doi.org/10.1186/s12870-023-04506-2 |
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| author | Wang Lu Yan Wang Yuan Shi Qin Liang Xiangyin Lu Deding Su Xin Xu Julien Pirrello Ying Gao Baowen Huang Zhengguo Li |
| author_facet | Wang Lu Yan Wang Yuan Shi Qin Liang Xiangyin Lu Deding Su Xin Xu Julien Pirrello Ying Gao Baowen Huang Zhengguo Li |
| author_sort | Wang Lu |
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| description | Abstract The SHI RELATED SEQUENCE (SRS) family plays a vital role in the development of multiple plant organs such as floral meristem determinacy, organ morphogenesis, and signal transduction. Nevertheless, there is little understanding of the biological significance of tomato SRS family at this point. Our research identified eight SlSRS family members and classified them into three subfamilies based on phylogenetics, conserved motifs, and characteristic domain analysis. The intraspecies and interspecies collinearity analysis revealed clues of SRS family evolution. Many cis-elements related to hormones, stresses, and plant development can be found in the promoter region of SlSRS genes. All of eight SlSRS proteins were located in the nucleus and possessed transcriptional activity, half of which were transcriptional activators, and the other half were transcriptional repressors. Except for SlSRS1, which showed high transcript accumulation in vegetative organs, most SlSRS genes expressed ubiquitously in all flower organs. In addition, all SlSRS genes could significantly respond to at least four different plant hormones. Further, expression of SlSRS genes were regulated by various abiotic stress conditions. In summary, we systematically analyzed and characterized the SlSRS family, reviewed the expression patterns and preliminarily investigated the protein function, and provided essential information for further functional research of the tomato SRS genes in the determination of reproductive floral organs and the development of plants, and possibly other plants. |
| format | Article |
| id | doaj-art-be3769f9f096498fbb4b6b01fedac1e4 |
| institution | DOAJ |
| issn | 1471-2229 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-be3769f9f096498fbb4b6b01fedac1e42025-08-20T03:16:40ZengBMCBMC Plant Biology1471-22292023-10-0123111710.1186/s12870-023-04506-2Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stressesWang Lu0Yan Wang1Yuan Shi2Qin Liang3Xiangyin Lu4Deding Su5Xin Xu6Julien Pirrello7Ying Gao8Baowen Huang9Zhengguo Li10Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityLaboratory of Plant Science Research, Fruit Genomics and Biotechnology, UMR5546, University of Toulouse, CNRS, UPS, Toulouse-NPKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing UniversityAbstract The SHI RELATED SEQUENCE (SRS) family plays a vital role in the development of multiple plant organs such as floral meristem determinacy, organ morphogenesis, and signal transduction. Nevertheless, there is little understanding of the biological significance of tomato SRS family at this point. Our research identified eight SlSRS family members and classified them into three subfamilies based on phylogenetics, conserved motifs, and characteristic domain analysis. The intraspecies and interspecies collinearity analysis revealed clues of SRS family evolution. Many cis-elements related to hormones, stresses, and plant development can be found in the promoter region of SlSRS genes. All of eight SlSRS proteins were located in the nucleus and possessed transcriptional activity, half of which were transcriptional activators, and the other half were transcriptional repressors. Except for SlSRS1, which showed high transcript accumulation in vegetative organs, most SlSRS genes expressed ubiquitously in all flower organs. In addition, all SlSRS genes could significantly respond to at least four different plant hormones. Further, expression of SlSRS genes were regulated by various abiotic stress conditions. In summary, we systematically analyzed and characterized the SlSRS family, reviewed the expression patterns and preliminarily investigated the protein function, and provided essential information for further functional research of the tomato SRS genes in the determination of reproductive floral organs and the development of plants, and possibly other plants.https://doi.org/10.1186/s12870-023-04506-2SRS familySolanum lycopersicumGenome-wide analysisGene expression analysisGene evolutionary analysis |
| spellingShingle | Wang Lu Yan Wang Yuan Shi Qin Liang Xiangyin Lu Deding Su Xin Xu Julien Pirrello Ying Gao Baowen Huang Zhengguo Li Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses BMC Plant Biology SRS family Solanum lycopersicum Genome-wide analysis Gene expression analysis Gene evolutionary analysis |
| title | Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses |
| title_full | Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses |
| title_fullStr | Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses |
| title_full_unstemmed | Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses |
| title_short | Identification of SRS transcription factor family in Solanum lycopersicum, and functional characterization of their responses to hormones and abiotic stresses |
| title_sort | identification of srs transcription factor family in solanum lycopersicum and functional characterization of their responses to hormones and abiotic stresses |
| topic | SRS family Solanum lycopersicum Genome-wide analysis Gene expression analysis Gene evolutionary analysis |
| url | https://doi.org/10.1186/s12870-023-04506-2 |
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