Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody.
Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transf...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183315&type=printable |
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| author | Veronika Chromikova Maria A Zaragoza Florian Krammer |
| author_facet | Veronika Chromikova Maria A Zaragoza Florian Krammer |
| author_sort | Veronika Chromikova |
| collection | DOAJ |
| description | Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool. |
| format | Article |
| id | doaj-art-bd64701417ab47e596b8ef876a89f7ce |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-bd64701417ab47e596b8ef876a89f7ce2025-08-20T02:46:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018331510.1371/journal.pone.0183315Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody.Veronika ChromikovaMaria A ZaragozaFlorian KrammerBecause of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183315&type=printable |
| spellingShingle | Veronika Chromikova Maria A Zaragoza Florian Krammer Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody. PLoS ONE |
| title | Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody. |
| title_full | Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody. |
| title_fullStr | Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody. |
| title_full_unstemmed | Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody. |
| title_short | Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody. |
| title_sort | generation of a serum free cho dg44 cell line stably producing a broadly protective anti influenza virus monoclonal antibody |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183315&type=printable |
| work_keys_str_mv | AT veronikachromikova generationofaserumfreechodg44celllinestablyproducingabroadlyprotectiveantiinfluenzavirusmonoclonalantibody AT mariaazaragoza generationofaserumfreechodg44celllinestablyproducingabroadlyprotectiveantiinfluenzavirusmonoclonalantibody AT floriankrammer generationofaserumfreechodg44celllinestablyproducingabroadlyprotectiveantiinfluenzavirusmonoclonalantibody |