The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique
The ethanolic (EE) and hydroalcoholic (HE) extracts of <i>Urochloa brizantha</i> concentrations were developed with the aim of evaluating their effect on rumen fermentation using an in vitro gas production technique. The EE and HE presented 3.62 and 5.38 mg protodioscin/mL, respectively....
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2024-08-01
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| author | Rafaela Scalise Xavier de Freitas Janaina Silveira da Silva Althieres José Furtado Flavio Perna Junior Alessandra Lopes de Oliveira Ives Cláudio da Silva Bueno |
| author_facet | Rafaela Scalise Xavier de Freitas Janaina Silveira da Silva Althieres José Furtado Flavio Perna Junior Alessandra Lopes de Oliveira Ives Cláudio da Silva Bueno |
| author_sort | Rafaela Scalise Xavier de Freitas |
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| description | The ethanolic (EE) and hydroalcoholic (HE) extracts of <i>Urochloa brizantha</i> concentrations were developed with the aim of evaluating their effect on rumen fermentation using an in vitro gas production technique. The EE and HE presented 3.62 and 5.38 mg protodioscin/mL, respectively. Ten treatments were evaluated in a completely randomized factorial arrangement (2 × 4 + 2), where the main effects were two extracts (EE and HE) and four levels (50, 100, 150, and 200 mL of extract/kg of DM) plus two controls: one positive (25 ppm of monensin–MON) and one (with no additives–CTL). The extract treatments (EXT, EE, and HE) reduced colonization time by 33.59% compared to the MON. IVDMD (<i>p</i> < 0.001) and IVOMD (<i>p</i> < 0.0001) were negatively affected by EXT addition when compared to CTL. Additionally, EXT reduced the proportion of propionic acid and increased the proportion of butyric acid in relation to CTL and MON treatments. Both EE and HE extracts of <i>U. brizantha</i> were able to alter rumen fermentation kinetic, with HE showing a higher concentration of protodioscin. Further research is needed to optimize extraction methodologies, comprehensively profile secondary compounds, and conduct trials with varying doses to effectively assess the viability of <i>U. brizantha</i> extract as an additive. |
| format | Article |
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| publishDate | 2024-08-01 |
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| spelling | doaj-art-bb8433cdf4fa439eabaf9301a307fa252025-08-20T01:55:27ZengMDPI AGFermentation2311-56372024-08-0110944710.3390/fermentation10090447The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro TechniqueRafaela Scalise Xavier de Freitas0Janaina Silveira da Silva1Althieres José Furtado2Flavio Perna Junior3Alessandra Lopes de Oliveira4Ives Cláudio da Silva Bueno5Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo/FZEA, Pirassununga CEP 13635-900, São Paulo, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo/FZEA, Pirassununga CEP 13635-900, São Paulo, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo/FZEA, Pirassununga CEP 13635-900, São Paulo, BrazilDepartment of Nutrition and Animal Production, College of Veterinary Medicine and Animal Science, University of São Paulo/FMVZ, Pirassununga CEP 13635-900, São Paulo, BrazilDepartment of Food Engineering, College of Animal Science and Food Engineering, University of São Paulo/FZEA, Pirassununga CEP 13635-900, São Paulo, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo/FZEA, Pirassununga CEP 13635-900, São Paulo, BrazilThe ethanolic (EE) and hydroalcoholic (HE) extracts of <i>Urochloa brizantha</i> concentrations were developed with the aim of evaluating their effect on rumen fermentation using an in vitro gas production technique. The EE and HE presented 3.62 and 5.38 mg protodioscin/mL, respectively. Ten treatments were evaluated in a completely randomized factorial arrangement (2 × 4 + 2), where the main effects were two extracts (EE and HE) and four levels (50, 100, 150, and 200 mL of extract/kg of DM) plus two controls: one positive (25 ppm of monensin–MON) and one (with no additives–CTL). The extract treatments (EXT, EE, and HE) reduced colonization time by 33.59% compared to the MON. IVDMD (<i>p</i> < 0.001) and IVOMD (<i>p</i> < 0.0001) were negatively affected by EXT addition when compared to CTL. Additionally, EXT reduced the proportion of propionic acid and increased the proportion of butyric acid in relation to CTL and MON treatments. Both EE and HE extracts of <i>U. brizantha</i> were able to alter rumen fermentation kinetic, with HE showing a higher concentration of protodioscin. Further research is needed to optimize extraction methodologies, comprehensively profile secondary compounds, and conduct trials with varying doses to effectively assess the viability of <i>U. brizantha</i> extract as an additive.https://www.mdpi.com/2311-5637/10/9/447degradabilityforagegas productionplant extractsaponin |
| spellingShingle | Rafaela Scalise Xavier de Freitas Janaina Silveira da Silva Althieres José Furtado Flavio Perna Junior Alessandra Lopes de Oliveira Ives Cláudio da Silva Bueno The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique Fermentation degradability forage gas production plant extract saponin |
| title | The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique |
| title_full | The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique |
| title_fullStr | The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique |
| title_full_unstemmed | The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique |
| title_short | The Production of Marandu Grass (<i>Urochloa brizantha</i>) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique |
| title_sort | production of marandu grass i urochloa brizantha i extracts as a natural modifier of rumen fermentation kinetics using an in vitro technique |
| topic | degradability forage gas production plant extract saponin |
| url | https://www.mdpi.com/2311-5637/10/9/447 |
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