Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice
Streptococcus oralis, a commensal oral Streptococcus, is known as an early colonizer of the tooth surface and causes opportunistic infections, such as bacterial endocarditis. However, its pathogenicity remains unclear. This study aimed to investigate the pathogenicity of S. oralis in vivo using a mo...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517425000379 |
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| author | Natsuno Nakamura Kota Iioka Hirobumi Morisaki Nobuo Okahashi Mie Kurosawa Haruka Fukamachi Shohei Matsui Takahiro Funatsu Hirotaka Kuwata Momoe Itsumi |
| author_facet | Natsuno Nakamura Kota Iioka Hirobumi Morisaki Nobuo Okahashi Mie Kurosawa Haruka Fukamachi Shohei Matsui Takahiro Funatsu Hirotaka Kuwata Momoe Itsumi |
| author_sort | Natsuno Nakamura |
| collection | DOAJ |
| description | Streptococcus oralis, a commensal oral Streptococcus, is known as an early colonizer of the tooth surface and causes opportunistic infections, such as bacterial endocarditis. However, its pathogenicity remains unclear. This study aimed to investigate the pathogenicity of S. oralis in vivo using a mouse model. To establish S. oralis-colonized mice, germ-free mice were orally infected with S. oralis. After colonization was confirmed, these infected mice were bred, and their offspring were used as S. oralis-colonized mice. S. oralis was detected only in the intestine of these mice, which exhibited soft stools but no significant inflammation in the examined tissues. Interestingly, S. oralis-colonized mice showed higher urination frequency. Bladder tissue analysis in S. oralis-colonized mice revealed atrophy, edema, fibrosis, and epithelial denudation. RNA sequencing analysis of the bladder in S. oralis-colonized mice indicated higher expression of genes related to chronic inflammation and extracellular matrix organization, and lower expression of genes related to anti-oxidative stress. In this study, we revealed that the commensal bacterium S. oralis induces chronic inflammation and fibrosis in the bladder of mice by intestinal colonization. Hence, our findings indicate that S. oralis has the potential to affect distal tissue beyond the oral cavity, potentially possessing a pathogenic factor involved in non-bacterial cystitis. This study highlights the potential impact of S. oralis on the urinary system of mice. |
| format | Article |
| id | doaj-art-b7dce048875f48d082b8a8e57019d0fe |
| institution | DOAJ |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-b7dce048875f48d082b8a8e57019d0fe2025-08-20T03:10:42ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-01810037510.1016/j.crmicr.2025.100375Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in miceNatsuno Nakamura0Kota Iioka1Hirobumi Morisaki2Nobuo Okahashi3Mie Kurosawa4Haruka Fukamachi5Shohei Matsui6Takahiro Funatsu7Hirotaka Kuwata8Momoe Itsumi9Department of Special Needs Dentistry, Division of Dentistry for Persons with Disabilities, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan; Department of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Department of Perioperative Medicine, Division of Anesthesiology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Special Needs Dentistry, Division of Medical and Dental Cooperative Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, JapanDepartment of Special Needs Dentistry, Division of Dentistry for Persons with Disabilities, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan; Department of Pediatric Dentistry, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Oral Microbiology and Immunology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Corresponding author.Streptococcus oralis, a commensal oral Streptococcus, is known as an early colonizer of the tooth surface and causes opportunistic infections, such as bacterial endocarditis. However, its pathogenicity remains unclear. This study aimed to investigate the pathogenicity of S. oralis in vivo using a mouse model. To establish S. oralis-colonized mice, germ-free mice were orally infected with S. oralis. After colonization was confirmed, these infected mice were bred, and their offspring were used as S. oralis-colonized mice. S. oralis was detected only in the intestine of these mice, which exhibited soft stools but no significant inflammation in the examined tissues. Interestingly, S. oralis-colonized mice showed higher urination frequency. Bladder tissue analysis in S. oralis-colonized mice revealed atrophy, edema, fibrosis, and epithelial denudation. RNA sequencing analysis of the bladder in S. oralis-colonized mice indicated higher expression of genes related to chronic inflammation and extracellular matrix organization, and lower expression of genes related to anti-oxidative stress. In this study, we revealed that the commensal bacterium S. oralis induces chronic inflammation and fibrosis in the bladder of mice by intestinal colonization. Hence, our findings indicate that S. oralis has the potential to affect distal tissue beyond the oral cavity, potentially possessing a pathogenic factor involved in non-bacterial cystitis. This study highlights the potential impact of S. oralis on the urinary system of mice.http://www.sciencedirect.com/science/article/pii/S2666517425000379Streptococcus oralisGerm-free miceBladderCystitisOxidative stressEctopic colonization |
| spellingShingle | Natsuno Nakamura Kota Iioka Hirobumi Morisaki Nobuo Okahashi Mie Kurosawa Haruka Fukamachi Shohei Matsui Takahiro Funatsu Hirotaka Kuwata Momoe Itsumi Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice Current Research in Microbial Sciences Streptococcus oralis Germ-free mice Bladder Cystitis Oxidative stress Ectopic colonization |
| title | Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice |
| title_full | Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice |
| title_fullStr | Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice |
| title_full_unstemmed | Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice |
| title_short | Pathogenic effects of Streptococcus oralis intestinal colonization on bladder health in mice |
| title_sort | pathogenic effects of streptococcus oralis intestinal colonization on bladder health in mice |
| topic | Streptococcus oralis Germ-free mice Bladder Cystitis Oxidative stress Ectopic colonization |
| url | http://www.sciencedirect.com/science/article/pii/S2666517425000379 |
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