Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques
Introduction: The development of CRISPR technology has ushered in a new era in molecular diagnostics whereby genetic material can be very accurately and efficiently detected. Therefore, the incorporation of these technologies into diagnostic protocols represents a paradigm shift in precision medicin...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | Journal of Clinical Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jcls.jcls_108_24 |
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| author | Samudrala Lahari Y. Sneha Chitti Sathwika Meghavath Tharun T. Rama Rao |
| author_facet | Samudrala Lahari Y. Sneha Chitti Sathwika Meghavath Tharun T. Rama Rao |
| author_sort | Samudrala Lahari |
| collection | DOAJ |
| description | Introduction:
The development of CRISPR technology has ushered in a new era in molecular diagnostics whereby genetic material can be very accurately and efficiently detected. Therefore, the incorporation of these technologies into diagnostic protocols represents a paradigm shift in precision medicine. This review aims to thoroughly assess the advancements and application of CRISPR into next-generation diagnostic tools, focusing on both existing and emerging technologies.
Methods:
The review presents CRISPR-based systems as historically moving from simple genome-editing tools into advanced diagnostic platforms, with emphasis on utilities that enhance diagnostic capability such as field-deployable assays and multiplex detection methodologies.
Results:
The review discusses CRISPRs-Cas13 and Cas12-and-Cas9 systems and their applications in the detection of infectious diseases, genetic disorders, and cancer. Emerging Techniques: The article points out emerging techniques using CRISPR for real-time monitoring and point-of-care diagnostics, showcasing the technology’s transformational capacity in diagnostic practice across various fields. Challenges and opportunities: The review outlines challenges and opportunities for CRISPR diagnostics, which include artificial intelligence configuration, multiplexing capability, and possibilities for personalized medicine.
Conclusion:
This review aims at highlighting the disrupting powers of CRISPR technologies on diagnostic systems toward the development of accurate, accessible, and inexpensive solutions for diagnostics in precision health. |
| format | Article |
| id | doaj-art-b2decb1adc6045d4babe8e5b2d2b806f |
| institution | DOAJ |
| issn | 2468-6859 2408-7408 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Clinical Sciences |
| spelling | doaj-art-b2decb1adc6045d4babe8e5b2d2b806f2025-08-20T03:09:12ZengWolters Kluwer Medknow PublicationsJournal of Clinical Sciences2468-68592408-74082025-01-01221364110.4103/jcls.jcls_108_24Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniquesSamudrala LahariY. SnehaChitti SathwikaMeghavath TharunT. Rama RaoIntroduction: The development of CRISPR technology has ushered in a new era in molecular diagnostics whereby genetic material can be very accurately and efficiently detected. Therefore, the incorporation of these technologies into diagnostic protocols represents a paradigm shift in precision medicine. This review aims to thoroughly assess the advancements and application of CRISPR into next-generation diagnostic tools, focusing on both existing and emerging technologies. Methods: The review presents CRISPR-based systems as historically moving from simple genome-editing tools into advanced diagnostic platforms, with emphasis on utilities that enhance diagnostic capability such as field-deployable assays and multiplex detection methodologies. Results: The review discusses CRISPRs-Cas13 and Cas12-and-Cas9 systems and their applications in the detection of infectious diseases, genetic disorders, and cancer. Emerging Techniques: The article points out emerging techniques using CRISPR for real-time monitoring and point-of-care diagnostics, showcasing the technology’s transformational capacity in diagnostic practice across various fields. Challenges and opportunities: The review outlines challenges and opportunities for CRISPR diagnostics, which include artificial intelligence configuration, multiplexing capability, and possibilities for personalized medicine. Conclusion: This review aims at highlighting the disrupting powers of CRISPR technologies on diagnostic systems toward the development of accurate, accessible, and inexpensive solutions for diagnostics in precision health.https://journals.lww.com/10.4103/jcls.jcls_108_24clustered regularly interspaced short palindromic repeatsprecision medicinegenomic-editingpoint-of-care diagnostics |
| spellingShingle | Samudrala Lahari Y. Sneha Chitti Sathwika Meghavath Tharun T. Rama Rao Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques Journal of Clinical Sciences clustered regularly interspaced short palindromic repeats precision medicine genomic-editing point-of-care diagnostics |
| title | Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques |
| title_full | Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques |
| title_fullStr | Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques |
| title_full_unstemmed | Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques |
| title_short | Innovations and implementations of clustered regularly interspaced short palindromic repeats in next-generation diagnostic tools: A review of current and emerging techniques |
| title_sort | innovations and implementations of clustered regularly interspaced short palindromic repeats in next generation diagnostic tools a review of current and emerging techniques |
| topic | clustered regularly interspaced short palindromic repeats precision medicine genomic-editing point-of-care diagnostics |
| url | https://journals.lww.com/10.4103/jcls.jcls_108_24 |
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