Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp.
The search for bioactive compounds for the treatment of several diseases has led to the study of endophytic fungi. Neoplastic diseases are among the most significant health concerns due to their high mortality rate, and there is a dearth of efficacious pharmaceutical agents for the treatment of canc...
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MDPI AG
2024-11-01
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| author | Gisele da Costa Ramos Ingryd Nayara de Farias Ramos Luciano Almeida Watanabe Luciana Almeida Watanabe Castro Alessandra Jackeline Guedes de Moraes Gleiciane Rodrigues dos Santos José Edson de Sousa Siqueira André Salim Khayat Andrey Moacir do Rosario Marinho Patrícia Santana Barbosa Marinho |
| author_facet | Gisele da Costa Ramos Ingryd Nayara de Farias Ramos Luciano Almeida Watanabe Luciana Almeida Watanabe Castro Alessandra Jackeline Guedes de Moraes Gleiciane Rodrigues dos Santos José Edson de Sousa Siqueira André Salim Khayat Andrey Moacir do Rosario Marinho Patrícia Santana Barbosa Marinho |
| author_sort | Gisele da Costa Ramos |
| collection | DOAJ |
| description | The search for bioactive compounds for the treatment of several diseases has led to the study of endophytic fungi. Neoplastic diseases are among the most significant health concerns due to their high mortality rate, and there is a dearth of efficacious pharmaceutical agents for the treatment of cancer. Gastric cancer is one of the most aggressive forms of cancer and is among those with the highest mortality rates in Brazil. Accordingly, the objective of this study was to identify compounds with cytotoxic activity from the mangrove-derived endophytic fungus <i>Trichoderma</i> sp. Isolation of the chemical compounds was conducted using chromatographic methods, while structural elucidation was achieved through the application of spectroscopic (NMR and UV) and spectrometric (MS) techniques. The fungus <i>Trichoderma</i> sp. was found to produce five distinct koninginins (A, B, C, E, and J). The organic phases of the extracts and isolated compounds were evaluated for their antimicrobial and cytotoxic potentials, respectively, through microdilution testing and the MTT method. In the cytotoxicity assay, both the AF extract and koninginin A demonstrated favorable outcomes, indicating their potential as promising anticancer therapeutic agents. |
| format | Article |
| id | doaj-art-b00fc3e139c24487b1561ebe9a4c32b5 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-b00fc3e139c24487b1561ebe9a4c32b52024-11-26T18:15:24ZengMDPI AGMolecules1420-30492024-11-012922527810.3390/molecules29225278Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp.Gisele da Costa Ramos0Ingryd Nayara de Farias Ramos1Luciano Almeida Watanabe2Luciana Almeida Watanabe Castro3Alessandra Jackeline Guedes de Moraes4Gleiciane Rodrigues dos Santos5José Edson de Sousa Siqueira6André Salim Khayat7Andrey Moacir do Rosario Marinho8Patrícia Santana Barbosa Marinho9Chemistry Graduate Program, Federal University of Pará, Rua Augusto Corrêa, 01, Belém 66075110, BrazilOncology Research Center, Federal University of Pará, Rua dos Mundurucus, 4487, Belém 66073005, BrazilChemistry Graduate Program, Federal University of Pará, Rua Augusto Corrêa, 01, Belém 66075110, BrazilEvolution Laboratory, Federal University of Pará, Alameda Leandro Ribeiro, Bragança 68600000, BrazilInstitute of Agricultural Sciences, Federal Rural University of the Amazon, Avenida Presidente Tancredo Neves, 2501, Belém 66077830, BrazilInstitute of Agricultural Sciences, Federal Rural University of the Amazon, Avenida Presidente Tancredo Neves, 2501, Belém 66077830, BrazilChemistry Graduate Program, Federal University of Pará, Rua Augusto Corrêa, 01, Belém 66075110, BrazilOncology Research Center, Federal University of Pará, Rua dos Mundurucus, 4487, Belém 66073005, BrazilChemistry Graduate Program, Federal University of Pará, Rua Augusto Corrêa, 01, Belém 66075110, BrazilChemistry Graduate Program, Federal University of Pará, Rua Augusto Corrêa, 01, Belém 66075110, BrazilThe search for bioactive compounds for the treatment of several diseases has led to the study of endophytic fungi. Neoplastic diseases are among the most significant health concerns due to their high mortality rate, and there is a dearth of efficacious pharmaceutical agents for the treatment of cancer. Gastric cancer is one of the most aggressive forms of cancer and is among those with the highest mortality rates in Brazil. Accordingly, the objective of this study was to identify compounds with cytotoxic activity from the mangrove-derived endophytic fungus <i>Trichoderma</i> sp. Isolation of the chemical compounds was conducted using chromatographic methods, while structural elucidation was achieved through the application of spectroscopic (NMR and UV) and spectrometric (MS) techniques. The fungus <i>Trichoderma</i> sp. was found to produce five distinct koninginins (A, B, C, E, and J). The organic phases of the extracts and isolated compounds were evaluated for their antimicrobial and cytotoxic potentials, respectively, through microdilution testing and the MTT method. In the cytotoxicity assay, both the AF extract and koninginin A demonstrated favorable outcomes, indicating their potential as promising anticancer therapeutic agents.https://www.mdpi.com/1420-3049/29/22/5278koningininscytotoxic activity<i>thichoderma</i>amazon |
| spellingShingle | Gisele da Costa Ramos Ingryd Nayara de Farias Ramos Luciano Almeida Watanabe Luciana Almeida Watanabe Castro Alessandra Jackeline Guedes de Moraes Gleiciane Rodrigues dos Santos José Edson de Sousa Siqueira André Salim Khayat Andrey Moacir do Rosario Marinho Patrícia Santana Barbosa Marinho Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp. Molecules koninginins cytotoxic activity <i>thichoderma</i> amazon |
| title | Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp. |
| title_full | Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp. |
| title_fullStr | Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp. |
| title_full_unstemmed | Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp. |
| title_short | Cytotoxic and Antibacterial Activity of Koninginins Isolated from the Mangrove-Derived Endophytic Fungus <i>Trichoderma</i> sp. |
| title_sort | cytotoxic and antibacterial activity of koninginins isolated from the mangrove derived endophytic fungus i trichoderma i sp |
| topic | koninginins cytotoxic activity <i>thichoderma</i> amazon |
| url | https://www.mdpi.com/1420-3049/29/22/5278 |
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