Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study

IntroductionThis study investigates the effects of polyphenol supplementation on gut microbiome composition and cardiovascular health in patients with ST-segment elevation myocardial infarction (STEMI).MethodsDouble-blind randomized control trial where participants received either polyphenol supplem...

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Main Authors: Argul Issilbayeva, Shynggys Sergazy, Azamat Zhashkeyev, Alexandr Gulyayev, Samat Kozhakhmetov, Zarina Shulgau, Madiyar Nurgaziyev, Ayaulym Nurgaziyeva, Sanzhar Zhetkenev, Nurislam Mukhanbetzhanov, Zharkyn Jarmukhanov, Zhanel Mukhanbetzhanova, Elizaveta Vinogradova, Zhaxybay Zhumadilov, Almagul Kushugulova, Mohamad Aljofan
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Medicine
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Online Access:https://www.frontiersin.org/articles/10.3389/fmed.2025.1522373/full
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author Argul Issilbayeva
Shynggys Sergazy
Azamat Zhashkeyev
Alexandr Gulyayev
Samat Kozhakhmetov
Zarina Shulgau
Madiyar Nurgaziyev
Ayaulym Nurgaziyeva
Sanzhar Zhetkenev
Nurislam Mukhanbetzhanov
Zharkyn Jarmukhanov
Zhanel Mukhanbetzhanova
Elizaveta Vinogradova
Zhaxybay Zhumadilov
Almagul Kushugulova
Mohamad Aljofan
Mohamad Aljofan
author_facet Argul Issilbayeva
Shynggys Sergazy
Azamat Zhashkeyev
Alexandr Gulyayev
Samat Kozhakhmetov
Zarina Shulgau
Madiyar Nurgaziyev
Ayaulym Nurgaziyeva
Sanzhar Zhetkenev
Nurislam Mukhanbetzhanov
Zharkyn Jarmukhanov
Zhanel Mukhanbetzhanova
Elizaveta Vinogradova
Zhaxybay Zhumadilov
Almagul Kushugulova
Mohamad Aljofan
Mohamad Aljofan
author_sort Argul Issilbayeva
collection DOAJ
description IntroductionThis study investigates the effects of polyphenol supplementation on gut microbiome composition and cardiovascular health in patients with ST-segment elevation myocardial infarction (STEMI).MethodsDouble-blind randomized control trial where participants received either polyphenol supplementation or placebo for 3 months, after which composition of the gut microbiome; clinical and laboratory parameters, including TMAO levels and oxidative stress levels, were assessed.ResultsThe stable TMAO levels (from 0.5 [0.2–0.9] to 0.4 [0.3–0.9] μmol, p > 0.05) were observed in the polyphenol group, compared to the increase observed in the placebo group (from 0.5 [0.3–0.6] to 0.7 [0.5–1.4] μmol, p < 0.001). Polyphenol supplementation significantly decreased the Firmicutes/Bacteroidetes ratio (p = 0.04) and increased beneficial bacteria such as Roseburia (p = 0.01), Agathobaculum sp. (p = 0.004), Alistipes finegoldii (p = 0.04) and Sellimonas (p = 0.002). Predicted metabolic pathways analysis supports potential mechanisms linking polyphenol intake to microbiome modulation and TMAO regulation.ConclusionOur findings demonstrate that polyphenol supplementation maintains stable TMAO levels by restructuring gut microbiome composition in STEMI patients, evidenced by a more focused microbiome with a significant increase in beneficial butyrate-producing bacteria (Roseburia, Agathobaculum sp., Alistipes finegoldii, and Sellimonas) and a decreased Firmicutes/Bacteroidetes ratio, suggesting microbiome-mediated cardioprotective effects. While promising,l our preliminary findings require further studies with larger cohorts and more advanced sequencing methods to establish their significance for cardiovascular health.Clinical trial registrationClinicalTrial.gov, identfier: NCT06573892.
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spelling doaj-art-accc4644c46d4d5f9a80eb329f1ff2a92025-08-20T02:33:20ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-05-011210.3389/fmed.2025.15223731522373Polyphenol-mediated microbiome modulation in STEMI patients: a pilot studyArgul Issilbayeva0Shynggys Sergazy1Azamat Zhashkeyev2Alexandr Gulyayev3Samat Kozhakhmetov4Zarina Shulgau5Madiyar Nurgaziyev6Ayaulym Nurgaziyeva7Sanzhar Zhetkenev8Nurislam Mukhanbetzhanov9Zharkyn Jarmukhanov10Zhanel Mukhanbetzhanova11Elizaveta Vinogradova12Zhaxybay Zhumadilov13Almagul Kushugulova14Mohamad Aljofan15Mohamad Aljofan16National Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanKaraganda Medical University, Karaganda, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanDepartment of Surgery, Nazarbayev University School of Medicine, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanNational Laboratory Astana, Center for Life Sciences, Nazarbayev University, Astana, KazakhstanDepartment of Biomedical Sciences, Nazarbayev University School of Medicine, Astana, KazakhstanIntroductionThis study investigates the effects of polyphenol supplementation on gut microbiome composition and cardiovascular health in patients with ST-segment elevation myocardial infarction (STEMI).MethodsDouble-blind randomized control trial where participants received either polyphenol supplementation or placebo for 3 months, after which composition of the gut microbiome; clinical and laboratory parameters, including TMAO levels and oxidative stress levels, were assessed.ResultsThe stable TMAO levels (from 0.5 [0.2–0.9] to 0.4 [0.3–0.9] μmol, p > 0.05) were observed in the polyphenol group, compared to the increase observed in the placebo group (from 0.5 [0.3–0.6] to 0.7 [0.5–1.4] μmol, p < 0.001). Polyphenol supplementation significantly decreased the Firmicutes/Bacteroidetes ratio (p = 0.04) and increased beneficial bacteria such as Roseburia (p = 0.01), Agathobaculum sp. (p = 0.004), Alistipes finegoldii (p = 0.04) and Sellimonas (p = 0.002). Predicted metabolic pathways analysis supports potential mechanisms linking polyphenol intake to microbiome modulation and TMAO regulation.ConclusionOur findings demonstrate that polyphenol supplementation maintains stable TMAO levels by restructuring gut microbiome composition in STEMI patients, evidenced by a more focused microbiome with a significant increase in beneficial butyrate-producing bacteria (Roseburia, Agathobaculum sp., Alistipes finegoldii, and Sellimonas) and a decreased Firmicutes/Bacteroidetes ratio, suggesting microbiome-mediated cardioprotective effects. While promising,l our preliminary findings require further studies with larger cohorts and more advanced sequencing methods to establish their significance for cardiovascular health.Clinical trial registrationClinicalTrial.gov, identfier: NCT06573892.https://www.frontiersin.org/articles/10.3389/fmed.2025.1522373/fullgut microbiomeSTEMIpolyphenol16S rRNA sequencingTMAO
spellingShingle Argul Issilbayeva
Shynggys Sergazy
Azamat Zhashkeyev
Alexandr Gulyayev
Samat Kozhakhmetov
Zarina Shulgau
Madiyar Nurgaziyev
Ayaulym Nurgaziyeva
Sanzhar Zhetkenev
Nurislam Mukhanbetzhanov
Zharkyn Jarmukhanov
Zhanel Mukhanbetzhanova
Elizaveta Vinogradova
Zhaxybay Zhumadilov
Almagul Kushugulova
Mohamad Aljofan
Mohamad Aljofan
Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study
Frontiers in Medicine
gut microbiome
STEMI
polyphenol
16S rRNA sequencing
TMAO
title Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study
title_full Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study
title_fullStr Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study
title_full_unstemmed Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study
title_short Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study
title_sort polyphenol mediated microbiome modulation in stemi patients a pilot study
topic gut microbiome
STEMI
polyphenol
16S rRNA sequencing
TMAO
url https://www.frontiersin.org/articles/10.3389/fmed.2025.1522373/full
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