Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana
Abstract Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machiner...
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Springer Nature
2010-08-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2010.53 |
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| author | Jelle Van Leene Jens Hollunder Dominique Eeckhout Geert Persiau Eveline Van De Slijke Hilde Stals Gert Van Isterdael Aurine Verkest Sandy Neirynck Yelle Buffel Stefanie De Bodt Steven Maere Kris Laukens Anne Pharazyn Paulo C G Ferreira Nubia Eloy Charlotte Renne Christian Meyer Jean‐Denis Faure Jens Steinbrenner Jim Beynon John C Larkin Yves Van de Peer Pierre Hilson Martin Kuiper Lieven De Veylder Harry Van Onckelen Dirk Inzé Erwin Witters Geert De Jaeger |
| author_facet | Jelle Van Leene Jens Hollunder Dominique Eeckhout Geert Persiau Eveline Van De Slijke Hilde Stals Gert Van Isterdael Aurine Verkest Sandy Neirynck Yelle Buffel Stefanie De Bodt Steven Maere Kris Laukens Anne Pharazyn Paulo C G Ferreira Nubia Eloy Charlotte Renne Christian Meyer Jean‐Denis Faure Jens Steinbrenner Jim Beynon John C Larkin Yves Van de Peer Pierre Hilson Martin Kuiper Lieven De Veylder Harry Van Onckelen Dirk Inzé Erwin Witters Geert De Jaeger |
| author_sort | Jelle Van Leene |
| collection | DOAJ |
| description | Abstract Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up‐ and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in‐depth biological interpretation demonstrated the hypothesis‐generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin‐dependent kinase (CDK)–cyclin complexes in plants. For the first time, inhibitory proteins of plant‐specific B‐type CDKs were discovered and the anaphase‐promoting complex was characterized and extended. Important conclusions were that mitotic A‐ and B‐type cyclins form complexes with the plant‐specific B‐type CDKs and not with CDKA;1, and that D‐type cyclins and S‐phase‐specific A‐type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK–cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants. |
| format | Article |
| id | doaj-art-ac7a8f0b60cd4d878f9bc09568d11dc5 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2010-08-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-ac7a8f0b60cd4d878f9bc09568d11dc52025-08-24T12:00:27ZengSpringer NatureMolecular Systems Biology1744-42922010-08-016111210.1038/msb.2010.53Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thalianaJelle Van Leene0Jens Hollunder1Dominique Eeckhout2Geert Persiau3Eveline Van De Slijke4Hilde Stals5Gert Van Isterdael6Aurine Verkest7Sandy Neirynck8Yelle Buffel9Stefanie De Bodt10Steven Maere11Kris Laukens12Anne Pharazyn13Paulo C G Ferreira14Nubia Eloy15Charlotte Renne16Christian Meyer17Jean‐Denis Faure18Jens Steinbrenner19Jim Beynon20John C Larkin21Yves Van de Peer22Pierre Hilson23Martin Kuiper24Lieven De Veylder25Harry Van Onckelen26Dirk Inzé27Erwin Witters28Geert De Jaeger29Department of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Mathematics and Computer Science, University of AntwerpDepartment of Biology, Center for Proteome Analysis and Mass Spectrometry, University of AntwerpInstituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de JaneiroDepartment of Plant Systems BiologyInstitut Jean‐Pierre Bourgin, INRA‐AgroParisTechInstitut Jean‐Pierre Bourgin, INRA‐AgroParisTechInstitut Jean‐Pierre Bourgin, INRA‐AgroParisTechSchool of Life Sciences, Warwick UniversitySchool of Life Sciences, Warwick UniversityDepartment of Biological Sciences, Louisiana State UniversityDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Plant Systems BiologyDepartment of Biology, Center for Proteome Analysis and Mass Spectrometry, University of AntwerpDepartment of Plant Systems BiologyDepartment of Biology, Center for Proteome Analysis and Mass Spectrometry, University of AntwerpDepartment of Plant Systems BiologyAbstract Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up‐ and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in‐depth biological interpretation demonstrated the hypothesis‐generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin‐dependent kinase (CDK)–cyclin complexes in plants. For the first time, inhibitory proteins of plant‐specific B‐type CDKs were discovered and the anaphase‐promoting complex was characterized and extended. Important conclusions were that mitotic A‐ and B‐type cyclins form complexes with the plant‐specific B‐type CDKs and not with CDKA;1, and that D‐type cyclins and S‐phase‐specific A‐type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK–cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.https://doi.org/10.1038/msb.2010.53Arabidopsis thalianacell cycleinteractomeprotein complexprotein interactions |
| spellingShingle | Jelle Van Leene Jens Hollunder Dominique Eeckhout Geert Persiau Eveline Van De Slijke Hilde Stals Gert Van Isterdael Aurine Verkest Sandy Neirynck Yelle Buffel Stefanie De Bodt Steven Maere Kris Laukens Anne Pharazyn Paulo C G Ferreira Nubia Eloy Charlotte Renne Christian Meyer Jean‐Denis Faure Jens Steinbrenner Jim Beynon John C Larkin Yves Van de Peer Pierre Hilson Martin Kuiper Lieven De Veylder Harry Van Onckelen Dirk Inzé Erwin Witters Geert De Jaeger Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana Molecular Systems Biology Arabidopsis thaliana cell cycle interactome protein complex protein interactions |
| title | Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana |
| title_full | Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana |
| title_fullStr | Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana |
| title_full_unstemmed | Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana |
| title_short | Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana |
| title_sort | targeted interactomics reveals a complex core cell cycle machinery in arabidopsis thaliana |
| topic | Arabidopsis thaliana cell cycle interactome protein complex protein interactions |
| url | https://doi.org/10.1038/msb.2010.53 |
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