Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology
<i>Background and Objectives</i>: Endothelial cells are essential to various therapeutic strategies for cardiovascular diseases. Developing efficient methods to generate large quantities of well-defined endothelial cells could improve cardiovascular treatment. This study explored the imp...
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2025-03-01
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| author | Do Hyun Kim Sang Hi Park Mi-yeon Kwon Chae-Yoon Lim Sun Hwa Park David W. Jang Se Hwan Hwang Sung Won Kim |
| author_facet | Do Hyun Kim Sang Hi Park Mi-yeon Kwon Chae-Yoon Lim Sun Hwa Park David W. Jang Se Hwan Hwang Sung Won Kim |
| author_sort | Do Hyun Kim |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Endothelial cells are essential to various therapeutic strategies for cardiovascular diseases. Developing efficient methods to generate large quantities of well-defined endothelial cells could improve cardiovascular treatment. This study explored the impact of lab-on-a-chip technology on the endothelial differentiation potential of mesenchymal stem cells derived from the human inferior nasal turbinate (hNTSCs). <i>Materials and Methods</i>: hNTSCs were isolated from five patients and divided into two groups: an experimental group subjected to lab-on-a-chip technology and a control group following two-dimensional differentiation protocols. The endothelial differentiation capacity of hNTSCs was assessed through histological examination and gene expression analysis. <i>Results</i>: Comparative evaluation of traditional differentiation methods and lab-on-a-chip technology indicated that hNTSCs expressed endothelial cell-specific markers, including CD34, KDR, CDH5, and CD31. Notably, CD31, CD34, and CDH5 exhibited significantly elevated expression levels in the lab-on-a-chip system. Additionally, cytokine analysis showed marked increases in IL-1a and IL-8 expression under lab-on-a-chip conditions compared to standard differentiation techniques. <i>Conclusions</i>: Lab-on-a-chip technology may enhance the differentiation of hNTSCs into endothelial cells with angiogenic potential, highlighting its promise for future cardiovascular regenerative applications. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-aa53e9973a784a5cb5445cbcb42d95fc2025-08-20T03:43:14ZengMDPI AGMedicina1010-660X1648-91442025-03-0161352810.3390/medicina61030528Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip TechnologyDo Hyun Kim0Sang Hi Park1Mi-yeon Kwon2Chae-Yoon Lim3Sun Hwa Park4David W. Jang5Se Hwan Hwang6Sung Won Kim7Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaInstitute of Clinical Medicine Research, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaInstitute of Clinical Medicine Research, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaInstitute of Clinical Medicine Research, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaPostech-Catholic Biomedical Engineering Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC 27710, USADepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea<i>Background and Objectives</i>: Endothelial cells are essential to various therapeutic strategies for cardiovascular diseases. Developing efficient methods to generate large quantities of well-defined endothelial cells could improve cardiovascular treatment. This study explored the impact of lab-on-a-chip technology on the endothelial differentiation potential of mesenchymal stem cells derived from the human inferior nasal turbinate (hNTSCs). <i>Materials and Methods</i>: hNTSCs were isolated from five patients and divided into two groups: an experimental group subjected to lab-on-a-chip technology and a control group following two-dimensional differentiation protocols. The endothelial differentiation capacity of hNTSCs was assessed through histological examination and gene expression analysis. <i>Results</i>: Comparative evaluation of traditional differentiation methods and lab-on-a-chip technology indicated that hNTSCs expressed endothelial cell-specific markers, including CD34, KDR, CDH5, and CD31. Notably, CD31, CD34, and CDH5 exhibited significantly elevated expression levels in the lab-on-a-chip system. Additionally, cytokine analysis showed marked increases in IL-1a and IL-8 expression under lab-on-a-chip conditions compared to standard differentiation techniques. <i>Conclusions</i>: Lab-on-a-chip technology may enhance the differentiation of hNTSCs into endothelial cells with angiogenic potential, highlighting its promise for future cardiovascular regenerative applications.https://www.mdpi.com/1648-9144/61/3/528microfluidicsendotheliumstem cellsculturehumans |
| spellingShingle | Do Hyun Kim Sang Hi Park Mi-yeon Kwon Chae-Yoon Lim Sun Hwa Park David W. Jang Se Hwan Hwang Sung Won Kim Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology Medicina microfluidics endothelium stem cells culture humans |
| title | Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology |
| title_full | Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology |
| title_fullStr | Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology |
| title_full_unstemmed | Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology |
| title_short | Enhancing Endothelial Differentiation of Mesenchymal Stem Cells Derived from Human Turbinates Using Lab-on-a-Chip Technology |
| title_sort | enhancing endothelial differentiation of mesenchymal stem cells derived from human turbinates using lab on a chip technology |
| topic | microfluidics endothelium stem cells culture humans |
| url | https://www.mdpi.com/1648-9144/61/3/528 |
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