Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.)
Abstract Seven in absentia proteins, which contain a conserved SINA domain, are involved in regulating various aspects of wheat (Triticum aestivum L.) growth and development, especially in response to environmental stresses. However, it is unclear whether TaSINA family members are involved in regula...
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| Format: | Article |
| Language: | English |
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Wiley
2024-09-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20480 |
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| author | Tao Chen Yongping Miao Fanli Jing Weidong Gao Yanyan Zhang Long Zhang Peipei Zhang Lijian Guo Delong Yang |
| author_facet | Tao Chen Yongping Miao Fanli Jing Weidong Gao Yanyan Zhang Long Zhang Peipei Zhang Lijian Guo Delong Yang |
| author_sort | Tao Chen |
| collection | DOAJ |
| description | Abstract Seven in absentia proteins, which contain a conserved SINA domain, are involved in regulating various aspects of wheat (Triticum aestivum L.) growth and development, especially in response to environmental stresses. However, it is unclear whether TaSINA family members are involved in regulating grain development until now. In this study, the expression pattern, genomic polymorphism, and relationship with grain‐related traits were analyzed for all TaSINA members. Most of the TaSINA genes identified showed higher expression levels in young wheat spikes or grains than other organs. The genomic polymorphism analysis revealed that at least 62 TaSINA genes had different haplotypes, where the haplotypes of five genes were significantly correlated with grain‐related traits. Kompetitive allele‐specific PCR markers were developed to confirm the single nucleotide polymorphisms in TaSINA101 and TaSINA109 among the five selected genes in a set of 292 wheat accessions. The TaSINA101‐Hap II and TaSINA109‐Hap II haplotypes had higher grain weight and width compared to TaSINA101‐Hap I and TaSINA109‐Hap I in at least three environments, respectively. The qRT‐PCR assays revealed that TaSINA101 was highly expressed in the palea shell, seed coat, and embryo in young wheat grains. The TaSINA101 protein was unevenly distributed in the nucleus when transiently expressed in the protoplast of wheat. Three homozygous TaSINA101 transgenic lines in rice (Oryza sativa L.) showed higher grain weight and size compared to the wild type. These findings provide valuable insight into the biological function and elite haplotype of TaSINA family genes in wheat grain development at a genomic‐wide level. |
| format | Article |
| id | doaj-art-9f1e4e2c3a7b44939f54bc0e8a68ef7c |
| institution | Kabale University |
| issn | 1940-3372 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj-art-9f1e4e2c3a7b44939f54bc0e8a68ef7c2024-11-17T09:46:07ZengWileyThe Plant Genome1940-33722024-09-01173n/an/a10.1002/tpg2.20480Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.)Tao Chen0Yongping Miao1Fanli Jing2Weidong Gao3Yanyan Zhang4Long Zhang5Peipei Zhang6Lijian Guo7Delong Yang8State Key Laboratory of Aridland Crop Science Gansu Agricultural University Lanzhou ChinaCollege of Life Science and Technology Gansu Agricultural University Lanzhou ChinaCollege of Life Science and Technology Gansu Agricultural University Lanzhou ChinaCollege of Life Science and Technology Gansu Agricultural University Lanzhou ChinaCollege of Life Science and Technology Gansu Agricultural University Lanzhou ChinaCollege of Life Science and Technology Gansu Agricultural University Lanzhou ChinaState Key Laboratory of Aridland Crop Science Gansu Agricultural University Lanzhou ChinaState Key Laboratory of Aridland Crop Science Gansu Agricultural University Lanzhou ChinaState Key Laboratory of Aridland Crop Science Gansu Agricultural University Lanzhou ChinaAbstract Seven in absentia proteins, which contain a conserved SINA domain, are involved in regulating various aspects of wheat (Triticum aestivum L.) growth and development, especially in response to environmental stresses. However, it is unclear whether TaSINA family members are involved in regulating grain development until now. In this study, the expression pattern, genomic polymorphism, and relationship with grain‐related traits were analyzed for all TaSINA members. Most of the TaSINA genes identified showed higher expression levels in young wheat spikes or grains than other organs. The genomic polymorphism analysis revealed that at least 62 TaSINA genes had different haplotypes, where the haplotypes of five genes were significantly correlated with grain‐related traits. Kompetitive allele‐specific PCR markers were developed to confirm the single nucleotide polymorphisms in TaSINA101 and TaSINA109 among the five selected genes in a set of 292 wheat accessions. The TaSINA101‐Hap II and TaSINA109‐Hap II haplotypes had higher grain weight and width compared to TaSINA101‐Hap I and TaSINA109‐Hap I in at least three environments, respectively. The qRT‐PCR assays revealed that TaSINA101 was highly expressed in the palea shell, seed coat, and embryo in young wheat grains. The TaSINA101 protein was unevenly distributed in the nucleus when transiently expressed in the protoplast of wheat. Three homozygous TaSINA101 transgenic lines in rice (Oryza sativa L.) showed higher grain weight and size compared to the wild type. These findings provide valuable insight into the biological function and elite haplotype of TaSINA family genes in wheat grain development at a genomic‐wide level.https://doi.org/10.1002/tpg2.20480 |
| spellingShingle | Tao Chen Yongping Miao Fanli Jing Weidong Gao Yanyan Zhang Long Zhang Peipei Zhang Lijian Guo Delong Yang Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.) The Plant Genome |
| title | Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.) |
| title_full | Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.) |
| title_fullStr | Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.) |
| title_short | Genomic‐wide analysis reveals seven in absentia genes regulating grain development in wheat (Triticum aestivum L.) |
| title_sort | genomic wide analysis reveals seven in absentia genes regulating grain development in wheat triticum aestivum l |
| url | https://doi.org/10.1002/tpg2.20480 |
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