Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor
Peanut (<i>Arachis hypogaea</i> L.) is an oilseed crop grown worldwide. Flavonoids have profound benefits for plant growth and development because of their powerful antioxidant properties. Seed vigor is an important indicator of seed quality. However, how flavonoids impact seed vigor for...
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2024-12-01
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| Series: | Antioxidants |
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| author | Fangping Gong Di Cao Zhuo Li Yi Fan Yaru Zhang Lin Zhang Kai Zhao Ding Qiu Zhongfeng Li Rui Ren Xingli Ma Xingguo Zhang Kunkun Zhao Dongmei Yin |
| author_facet | Fangping Gong Di Cao Zhuo Li Yi Fan Yaru Zhang Lin Zhang Kai Zhao Ding Qiu Zhongfeng Li Rui Ren Xingli Ma Xingguo Zhang Kunkun Zhao Dongmei Yin |
| author_sort | Fangping Gong |
| collection | DOAJ |
| description | Peanut (<i>Arachis hypogaea</i> L.) is an oilseed crop grown worldwide. Flavonoids have profound benefits for plant growth and development because of their powerful antioxidant properties. Seed vigor is an important indicator of seed quality. However, how flavonoids impact seed vigor formation in large-seed peanuts is still poorly understood. Here, we profiled flavonoids, phytohormones, and transcriptomes of developing seeds of large-seed peanut varieties with low (ZP06) and high (H8107) seed vigor. A total of 165 flavonoids were identified, 51 of which were differentially accumulated in ZP06 and H8107. Lower levels of dihydromyricetin (0.28 times) and hesperetin-7-O-glucoside (0.26 times) were observed in ZP06 seeds than in H8107. All flavonoid biosynthesis structural genes were down-regulated in ZP06. The different hormone levels found in ZP06 and H8107 seeds could be associated with the expression of flavonoid biosynthesis genes via MYB and bHLH transcription factors. Dihydromyricetin could relate to ZP06′s poor seed vigor by impacting its seed antioxidant properties. Thus, the presence of flavonoids in large-seed peanuts could contribute to their physiological quality and germination potential through controlling the accumulation of reactive oxygen species to improve seed antioxidant properties. |
| format | Article |
| id | doaj-art-9be1eb60ee1343b499be2821bcd24acc |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-9be1eb60ee1343b499be2821bcd24acc2024-12-27T14:06:45ZengMDPI AGAntioxidants2076-39212024-12-011312149710.3390/antiox13121497Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed VigorFangping Gong0Di Cao1Zhuo Li2Yi Fan3Yaru Zhang4Lin Zhang5Kai Zhao6Ding Qiu7Zhongfeng Li8Rui Ren9Xingli Ma10Xingguo Zhang11Kunkun Zhao12Dongmei Yin13College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450002, ChinaPeanut (<i>Arachis hypogaea</i> L.) is an oilseed crop grown worldwide. Flavonoids have profound benefits for plant growth and development because of their powerful antioxidant properties. Seed vigor is an important indicator of seed quality. However, how flavonoids impact seed vigor formation in large-seed peanuts is still poorly understood. Here, we profiled flavonoids, phytohormones, and transcriptomes of developing seeds of large-seed peanut varieties with low (ZP06) and high (H8107) seed vigor. A total of 165 flavonoids were identified, 51 of which were differentially accumulated in ZP06 and H8107. Lower levels of dihydromyricetin (0.28 times) and hesperetin-7-O-glucoside (0.26 times) were observed in ZP06 seeds than in H8107. All flavonoid biosynthesis structural genes were down-regulated in ZP06. The different hormone levels found in ZP06 and H8107 seeds could be associated with the expression of flavonoid biosynthesis genes via MYB and bHLH transcription factors. Dihydromyricetin could relate to ZP06′s poor seed vigor by impacting its seed antioxidant properties. Thus, the presence of flavonoids in large-seed peanuts could contribute to their physiological quality and germination potential through controlling the accumulation of reactive oxygen species to improve seed antioxidant properties.https://www.mdpi.com/2076-3921/13/12/1497peanutlarge seedflavonoidseed vigorphytohormone |
| spellingShingle | Fangping Gong Di Cao Zhuo Li Yi Fan Yaru Zhang Lin Zhang Kai Zhao Ding Qiu Zhongfeng Li Rui Ren Xingli Ma Xingguo Zhang Kunkun Zhao Dongmei Yin Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor Antioxidants peanut large seed flavonoid seed vigor phytohormone |
| title | Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor |
| title_full | Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor |
| title_fullStr | Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor |
| title_full_unstemmed | Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor |
| title_short | Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor |
| title_sort | metabolomic and transcriptomic analysis reveals flavonoid mediated regulation of seed antioxidant properties in peanut seed vigor |
| topic | peanut large seed flavonoid seed vigor phytohormone |
| url | https://www.mdpi.com/2076-3921/13/12/1497 |
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