In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate
Abstract The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into n...
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Sociedade Brasileira de Pesquisa Odontológica
2024-12-01
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| Series: | Brazilian Oral Research |
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| author | Carolina Ruis Ferrari Karolyne Sayuri de Araujo Kitamoto Vinicius Taioqui Pelá Éven Akemi Taira Tamara Teodoro Araújo Larissa Tercilia Grizzo Thomassian Flávio Henrique-Silva Juliano Pelim Pessan Marília Afonso Rabelo Buzalaf |
| author_facet | Carolina Ruis Ferrari Karolyne Sayuri de Araujo Kitamoto Vinicius Taioqui Pelá Éven Akemi Taira Tamara Teodoro Araújo Larissa Tercilia Grizzo Thomassian Flávio Henrique-Silva Juliano Pelim Pessan Marília Afonso Rabelo Buzalaf |
| author_sort | Carolina Ruis Ferrari |
| collection | DOAJ |
| description | Abstract The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into nine groups (n/group=15) according to the following treatments: Deionized water; Commercial solution (Elmex Erosion ProtectionTM); 0.1 mg/mL CaneCPI-5; 0.5% TMP; 1.0% TMP; 3.0% TMP; 0.1 mg/mL CaneCPI-5+0.5% TMP; 0.1 mg/mL CaneCPI-5+1.0%TMP; and 0.1 mg/mL CaneCPI-5+3.0%TMP. The specimens were treated with the respective solutions for 2 h, followed by acquired enamel pellicle formation for 2 h and exposure to 0.65% citric acid (CA) for 1 min. These procedures were repeated once a day for three consecutive days. Demineralization was assessed by the percentage change in surface hardness (%CSH) and calcium release into CA, analyzed by the Arsenazo III method. The data were evaluated using Kruskal-Wallis/Dunn's tests. Regarding %CSH, CaneCPI-5+3.0%TMP was the most effective treatment when compared to the CaneCPI-5 group alone. As for calcium release into CA, the CaneCPI-5+0.5% TMP and CaneCPI-5 groups (both with lower calcium release) did not significantly differ from the commercial solution. In conclusion, combination of CaneCPI-5 with TMP enhances the protective potential against initial enamel erosion in vitro. |
| format | Article |
| id | doaj-art-9bb23c9e845541009b1c6e8950221d6b |
| institution | Kabale University |
| issn | 1807-3107 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj-art-9bb23c9e845541009b1c6e8950221d6b2024-12-17T08:05:23ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072024-12-013810.1590/1807-3107bor-2024.vol38.0124In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphateCarolina Ruis Ferrarihttps://orcid.org/0000-0002-3997-043XKarolyne Sayuri de Araujo Kitamotohttps://orcid.org/0000-0003-2569-2624Vinicius Taioqui Peláhttps://orcid.org/0000-0001-7933-4422Éven Akemi Tairahttps://orcid.org/0000-0003-0853-1335Tamara Teodoro Araújohttps://orcid.org/0000-0002-7560-9051Larissa Tercilia Grizzo Thomassianhttps://orcid.org/0000-0002-1469-5224Flávio Henrique-Silvahttps://orcid.org/0000-0003-3329-4597Juliano Pelim Pessanhttps://orcid.org/0000-0002-1550-3933Marília Afonso Rabelo Buzalafhttps://orcid.org/0000-0002-5985-3951Abstract The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into nine groups (n/group=15) according to the following treatments: Deionized water; Commercial solution (Elmex Erosion ProtectionTM); 0.1 mg/mL CaneCPI-5; 0.5% TMP; 1.0% TMP; 3.0% TMP; 0.1 mg/mL CaneCPI-5+0.5% TMP; 0.1 mg/mL CaneCPI-5+1.0%TMP; and 0.1 mg/mL CaneCPI-5+3.0%TMP. The specimens were treated with the respective solutions for 2 h, followed by acquired enamel pellicle formation for 2 h and exposure to 0.65% citric acid (CA) for 1 min. These procedures were repeated once a day for three consecutive days. Demineralization was assessed by the percentage change in surface hardness (%CSH) and calcium release into CA, analyzed by the Arsenazo III method. The data were evaluated using Kruskal-Wallis/Dunn's tests. Regarding %CSH, CaneCPI-5+3.0%TMP was the most effective treatment when compared to the CaneCPI-5 group alone. As for calcium release into CA, the CaneCPI-5+0.5% TMP and CaneCPI-5 groups (both with lower calcium release) did not significantly differ from the commercial solution. In conclusion, combination of CaneCPI-5 with TMP enhances the protective potential against initial enamel erosion in vitro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242024000100705&lng=en&tlng=enCystatinsDental PellicleSalivaTooth Erosion |
| spellingShingle | Carolina Ruis Ferrari Karolyne Sayuri de Araujo Kitamoto Vinicius Taioqui Pelá Éven Akemi Taira Tamara Teodoro Araújo Larissa Tercilia Grizzo Thomassian Flávio Henrique-Silva Juliano Pelim Pessan Marília Afonso Rabelo Buzalaf In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate Brazilian Oral Research Cystatins Dental Pellicle Saliva Tooth Erosion |
| title | In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate |
| title_full | In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate |
| title_fullStr | In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate |
| title_full_unstemmed | In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate |
| title_short | In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate |
| title_sort | in vitro reduction of enamel erosion by sugarcane derived cystatin associated with sodium trimetaphosphate |
| topic | Cystatins Dental Pellicle Saliva Tooth Erosion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242024000100705&lng=en&tlng=en |
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