The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells
Atractylis flava Desf. (AF) is common plant that is widely used for its anti- inflammatory and antioxidant properties. The purpose of this study was, therefore, to evaluate the cytotoxic effect and the molecular basis of antioxidant and anti-inflammatory effects of the ethyl acetate extract (AFEAE)...
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2024-09-01
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| Series: | Current Issues in Pharmacy and Medical Sciences |
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| Online Access: | https://doi.org/10.2478/cipms-2024-0022 |
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| author | Melakhessou Mohamed Akram Eddine Marref Salah Zahra Doumandji Ramia Safar Marref Cherine Imene Becheker Olivier Joubert |
| author_facet | Melakhessou Mohamed Akram Eddine Marref Salah Zahra Doumandji Ramia Safar Marref Cherine Imene Becheker Olivier Joubert |
| author_sort | Melakhessou Mohamed Akram |
| collection | DOAJ |
| description | Atractylis flava Desf. (AF) is common plant that is widely used for its anti- inflammatory and antioxidant properties. The purpose of this study was, therefore, to evaluate the cytotoxic effect and the molecular basis of antioxidant and anti-inflammatory effects of the ethyl acetate extract (AFEAE) obtained from the whole plant A. flava. This was accomplished through the use of NR8383 alveolar rat macrophage cells. Cultures of alveolar rat macrophage cells were treated with AFEAE (25–800 μg/mL), and cell viability was determined via WST-1 and LDH tests. In turn, the gene expression levels of nuclear factor κB (NF-κB), tumor necrosis factor-alpha (TNFα), interleukin 1 beta (IL1-β), interleukin 6 (IL-6), mitochondrial dynamin like GTPase (OPA1), Succinate dehydrogenase complex subunit A (SDHA) and neutrophil cytosolic factor 1 (NCF1) were assessed by applying RT-qPCR. The results show that ethyl acetate extracts of A. flava have non-cytotoxic effects, and the gene expression analysis demonstrates that AFEAF extracts generate significant downregulation of NF-κB, TNFα, IL-1 β, IL-6, NCF1, OPA1 and SDHA, compared to untreated cells. This study reveals that Atractylis flava ethyl acetate extract administration may be considered as a potential therapeutic strategy for inflammatory related diseases. |
| format | Article |
| id | doaj-art-94d8f310d369463a938cb66b20b15731 |
| institution | Kabale University |
| issn | 2300-6676 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Sciendo |
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| series | Current Issues in Pharmacy and Medical Sciences |
| spelling | doaj-art-94d8f310d369463a938cb66b20b157312024-11-11T08:37:26ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2300-66762024-09-0137313814210.2478/cipms-2024-0022The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cellsMelakhessou Mohamed Akram0Eddine Marref Salah1Zahra Doumandji2Ramia Safar3Marref Cherine4Imene Becheker5Olivier Joubert6Laboratoire de Biotechnologie des Molécules Bioactives et de la Physiopathologie Cellulaire (LBMBPC), Batna, AlgeriaInstitut Jean Lamour, CNRS-Université de Lorraine, Nancy, FranceInstitut Jean Lamour, CNRS-Université de Lorraine, Nancy, FranceNutrition-Genetics-Environmental Risk Exposure, University of Lorraine, Nancy, FranceLaboratoire Biologie, Eau et Environnement (LBEE), Faculté SNTV-STU, AlgeriaLaboratoire de recherche Interactions, Biodiversité, Écosystèmes et Biotechnologie (LRIBEB), AlgeriaInstitut Jean Lamour, CNRS-Université de Lorraine, Nancy, FranceAtractylis flava Desf. (AF) is common plant that is widely used for its anti- inflammatory and antioxidant properties. The purpose of this study was, therefore, to evaluate the cytotoxic effect and the molecular basis of antioxidant and anti-inflammatory effects of the ethyl acetate extract (AFEAE) obtained from the whole plant A. flava. This was accomplished through the use of NR8383 alveolar rat macrophage cells. Cultures of alveolar rat macrophage cells were treated with AFEAE (25–800 μg/mL), and cell viability was determined via WST-1 and LDH tests. In turn, the gene expression levels of nuclear factor κB (NF-κB), tumor necrosis factor-alpha (TNFα), interleukin 1 beta (IL1-β), interleukin 6 (IL-6), mitochondrial dynamin like GTPase (OPA1), Succinate dehydrogenase complex subunit A (SDHA) and neutrophil cytosolic factor 1 (NCF1) were assessed by applying RT-qPCR. The results show that ethyl acetate extracts of A. flava have non-cytotoxic effects, and the gene expression analysis demonstrates that AFEAF extracts generate significant downregulation of NF-κB, TNFα, IL-1 β, IL-6, NCF1, OPA1 and SDHA, compared to untreated cells. This study reveals that Atractylis flava ethyl acetate extract administration may be considered as a potential therapeutic strategy for inflammatory related diseases.https://doi.org/10.2478/cipms-2024-0022atractylis flava desfnr8383nf-κbtnf-αil-1βil-6ncf1opa1sdha |
| spellingShingle | Melakhessou Mohamed Akram Eddine Marref Salah Zahra Doumandji Ramia Safar Marref Cherine Imene Becheker Olivier Joubert The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells Current Issues in Pharmacy and Medical Sciences atractylis flava desf nr8383 nf-κb tnf-α il-1β il-6 ncf1 opa1 sdha |
| title | The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells |
| title_full | The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells |
| title_fullStr | The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells |
| title_full_unstemmed | The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells |
| title_short | The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells |
| title_sort | effect of ethyl acetate extract from atractylis flava desf on the gene expression of pro inflammatory cytokines and oxidative stress markers in nr8383 alveolar rat macrophage cells |
| topic | atractylis flava desf nr8383 nf-κb tnf-α il-1β il-6 ncf1 opa1 sdha |
| url | https://doi.org/10.2478/cipms-2024-0022 |
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